Invitrogen

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP

Rabbit IgG (H+L) Secondary Antibody in Western Blot (WB)

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Rabbit IgG (H+L) Secondary Antibody (31460) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1) and Hep G2 (Lane 2). The blots were probed with Anti-CacyBP Rabbit Polyclonal Antibody (Product # 720326, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # 31460) at dilutions 1:10,000 (Fig. 1), 1:100,000 (Fig. 2) and 1:200,000 (Fig. 3). A 30 kDa b... View More

Rabbit IgG (H+L) Secondary Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Rabbit IgG (H+L) Secondary Antibody in Immunoprecipitation (IP)

Rabbit IgG (H+L) Secondary Antibody in ELISA (ELISA)

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Product Details

31460

Applications

Tested Dilution

Western Blot (WB)

1:10,000-1:200,000

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

ELISA (ELISA)

1:5,000-1:10,000

Immunoprecipitation (IP)

1:500-1:5,000

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Purified Rabbit IgG, whole molecule

Conjugate

HRP HRP HRP

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 15mg/mL BSA, 50mM sucrose

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient

RRID

AB_228341

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Product # 31460 has been successfully used in Western blot, IHC and IP applications.

Product # 31460 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species and with SuperBlock® Blocking Buffers.

Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.

Reconstitute with 2.0 mL of distilled water (0.8 mg/mL after restoration).

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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