Invitrogen

Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight™ 488

Mouse IgG (H+L) Secondary Antibody in Immunocytochemistry (ICC/IF)

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Mouse IgG (H+L) Secondary Antibody (35502) in ICC/IF

Immunofluorescence analysis of Goat anti-Mouse IgG (H+L) Secondary Antibody DyLight® 488 conjugate was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL Mouse primary antibody ... View More

Mouse IgG (H+L) Secondary Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Mouse IgG (H+L) Secondary Antibody in Flow Cytometry (Flow)

Mouse IgG (H+L) Secondary Antibody in Western Blot (WB)

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Product Details

35502

Applications

Tested Dilution

Western Blot (WB)

1:5,000-1:20,000

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunocytochemistry (ICC/IF)

1 µg/mL

Flow Cytometry (Flow)

1:25 - 1:100

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Purified Mouse IgG, whole molecule

Conjugate

DyLight™ 488 DyLight™ 488 DyLight™ 488

Excitation/Emission Max

492/519 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient

RRID

AB_844397

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Product # 35502 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.

Product # 35502 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 488 Amax= 493 nm; Emax= 518 nm. Mole Dye/Mole Protein Ratio is lot-dependent.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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