Invitrogen

GSK3B Monoclonal Antibody (21A)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA1-7621

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
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Immunohistochemistry (IHC)

Assay-dependent
-

Flow Cytometry (Flow)

Assay-dependent
-

ELISA (ELISA)

1:1,000
-

Immunoprecipitation (IP)

5-10 µg
-
Product Specifications

Species Reactivity

Rat, Xenopus, Human

Published species

Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

21A

Immunogen

Purified recombinant Xenopus laevis protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.2

Contains

0.01% sodium azide

Storage conditions

4°C short term, -80°C long term

Shipping conditions

Dry ice

RRID

AB_1074718

Product Specific Information

MA1-7621 detects GSK3 Beta from human, rat, and Xenopus laevis samples.

MA1-7621 has been successfully used in ELISA, Western blot, Flow cytometry, Immunoprecipitation and Immunohistochemistry procedures.

The MA1-7621 immunogen is a purified recombinant Xenopus laevis protein.

Store at 4ºC short term. For extended storage, keep at -80ºC.

Target Information

GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development and body pattern formation. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. GSK3B is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. GSK3B is also involved in the Hedgehog, Notch, and insulin signaling pathways.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: EC 2.7.11.26; FA; Factor A; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK 3B; GSK-3 beta; GSK-3 beta GSK3B; GSK-3b; GSK3 beta; GSK3beta isoform; intracellular kinase; Serine/threonine-protein kinase GSK3B; Xgsk-3 protein

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Gene Aliases: gsk-3; gsk3; gsk3-beta; GSK3B; gsk3beta; XELAEV_180112401mg; Xgsk-3

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UniProt ID: (Human) P49841, (Rat) P18266, (Xenopus) Q91757

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Entrez Gene ID: (Human) 2932, (Rat) 84027, (Xenopus) 399097

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Function(s)
RNA polymerase II transcription factor binding p53 binding protein kinase activity protein serine/threonine kinase activity integrin binding protein binding ATP binding beta-catenin binding kinase activity protein kinase binding ubiquitin protein ligase binding protein kinase A catalytic subunit binding ionotropic glutamate receptor binding tau protein binding tau-protein kinase activity NF-kappaB binding transcription factor binding non-receptor serine/threonine protein kinase
Process(es)
re-entry into mitotic cell cycle epithelial to mesenchymal transition positive regulation of cell-matrix adhesion glycogen metabolic process regulation of gene expression by genetic imprinting protein phosphorylation protein export from nucleus ER overload response dopamine receptor signaling pathway axonogenesis myoblast fusion aging circadian rhythm organ morphogenesis negative regulation of cardiac muscle hypertrophy positive regulation of peptidyl-threonine phosphorylation positive regulation of mitochondrial membrane potential negative regulation of neuron maturation response to activity Wnt signaling pathway cell migration peptidyl-serine phosphorylation peptidyl-threonine phosphorylation hippocampus development establishment of cell polarity negative regulation of protein complex assembly positive regulation of protein complex assembly negative regulation of protein binding positive regulation of protein binding response to estradiol positive regulation of proteasomal ubiquitin-dependent protein catabolic process response to insulin regulation of microtubule-based process positive regulation of peptidyl-serine phosphorylation negative regulation of smooth muscle cell apoptotic process protein localization to microtubule intracellular signal transduction cellular response to hepatocyte growth factor stimulus cellular response to interleukin-3 cellular response to erythropoietin response to drug negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of MAP kinase activity positive regulation of neuron apoptotic process positive regulation of GTPase activity hypermethylation of CpG island canonical Wnt signaling pathway involved in positive regulation of apoptotic process fat cell differentiation negative regulation of glycogen biosynthetic process positive regulation of protein catabolic process positive regulation of axon extension positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation positive regulation of protein export from nucleus regulation of neuronal synaptic plasticity positive regulation of smooth muscle cell proliferation negative regulation of dendrite morphogenesis negative regulation of nitric-oxide synthase activity negative regulation of NFAT protein import into nucleus canonical Wnt signaling pathway intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide superior temporal gyrus development cellular response to mechanical stimulus cellular response to iron(II) ion cellular response to lithium ion response to epinephrine negative regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway in absence of ligand chemical synaptic transmission, postsynaptic regulation of cellular response to heat negative regulation of protein localization to nucleus positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway positive regulation of neuron death response to L-glutamate cellular response to dopamine negative regulation of dopaminergic neuron differentiation beta-catenin destruction complex assembly beta-catenin destruction complex disassembly response to insulin-like growth factor stimulus response to ultrasound response to angiotensin negative regulation of type B pancreatic cell development positive regulation of excitatory postsynaptic potential negative regulation of glycogen (starch) synthase activity positive regulation of DNA biosynthetic process positive regulation of stem cell differentiation negative regulation of neuron migration establishment or maintenance of cell polarity response to lithium ion regulation of neuron projection development negative regulation of neuron projection development myotube differentiation phosphorylation positive regulation of apoptotic process
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