Invitrogen

GPCR/G-protein complex-stabilizing scFv Recombinant Mouse Monoclonal Antibody (scFv16)

GPCR/G-protein complex-stabilizing scFv Antibody

FIGURE: 1 / 2

GPCR/G-protein complex-stabilizing scFv Antibody (703976-1MG)

A tagged GPCR was used to pull down a GPCR-heterotrimeric G protein complex that is stabilized by scFv16 (Product # 703976). A) Size exclusion chromatography (SEC) chromatogram of the purified complex. B) Highlighted sample from SEC was run on SDS-PAGE gel and Coomassie stained. All components of the active complex are present. C) Direct visualization of GPCR-G protein complex by negative stain TEM showing features characteristic for active GPCR complexes that are intact. Data courtesy Dr. David M. Thal, Monash Institute of Pharmaceutical ... View More

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Product Details

703976-1MG

Applications

Tested Dilution

Publications

Not applicable (N/A)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

scFv16

Immunogen

Rhodopsin/Gi1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.0 mg/mL

Purification

Affinity chromatography

Storage buffer

25mM HEPES, pH 7.5, with 10% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2896321

Product Specific Information

The scFv antibody stabilizes GPCR/G-protein complexes for cryo-EM of active structures. It recognizes an interface between G alpha and G beta/gamma subunits, and confers resistance to nucleotide exchange-triggered dissociation of the heterotrimeric G-protein. In addition to members of the Gi/o family, this antibody fragment can also be applied to chimeric variants and stabilize other G-protein subtypes including Gs complexes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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