Invitrogen

GFAP Monoclonal Antibody (GA5), eFluor™ 615, eBioscience™

GFAP Antibody in Immunocytochemistry (ICC/IF)

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GFAP Antibody (42-9892-82) in ICC/IF

Immunocytochemistry on fixed and permeabilized C6 cells using 10 µg/mL of Mouse IgG1 K Isotype Control eFluor® 615 (left) or 10 µg/mL of Anti-Glial Fibrillary Acidic Protein eFluor® 615 (right). Nuclei are counterstained with DAPI.

GFAP Antibody in Immunohistochemistry (IHC)

GFAP Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))

GFAP Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

42-9892-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 16 publications 16 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

10 µg/mL

View 5 publications 5 publications

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Pig, Rabbit, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GA5

Conjugate

eFluor™ 615 eFluor™ 615 eFluor™ 615

Excitation/Emission Max

595/614 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10804394

Product Specific Information

Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predominant form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors.

Applications Reported: This GA5 antibody has been reported for use in immunocytochemical (ICC) and immunohistochemical staining of frozen tissue sections (IHC-F).

Applications Tested: This GA5 antibody has been tested by immunocytochemistry on fixed and permeabilized C6 cells at less than or equal to 10 µg/mL. This product has not been validated for flow cytometric analysis.

Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.

Excitation: 595 nm; Emission: 615 nm.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Direct implantation of hair-follicle-associated pluripotent (HAP) stem cells repairs intracerebral hemorrhage and reduces neuroinflammation in mouse model.

PloS one

Obara K,Shirai K,Hamada Y,Arakawa N,Hasegawa A,Takaoka N,Aki R,Hoffman RM,Amoh Y

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunocytochemistry

Tue Jan 17 00:00:00 EST 2023

Apolipoprotein E ε4 disrupts oligodendrocyte differentiation by interfering with astrocyte-derived lipid transport.

Journal of neurochemistry

Mok KK,Yeung SH,Cheng GW,Ma IW,Lee RH,Herrup K,Tse KH

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Sat Apr 01 00:00:00 EDT 2023

Altered Tau Kinase Activity in rTg4510 Mice after a Single Interfaced CHIMERA Traumatic Brain Injury.

International journal of molecular sciences

Cheng WH,Cheung H,Kang A,Fan J,Cooper J,Anwer M,Barron C,Wilkinson A,Hu G,Yue J,Cripton PA,Vocadlo DJ,Wellington CL

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Mon May 29 00:00:00 EDT 2023

Light-sheet mesoscopy with the Mesolens provides fast sub-cellular resolution imaging throughout large tissue volumes.

iScience

Battistella E,Schniete J,Wesencraft K,Quintana JF,McConnell G

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Fri Sep 16 00:00:00 EDT 2022

The spatial transcriptomic landscape of non-small cell lung cancer brain metastasis.

Nature communications

Zhang Q,Abdo R,Iosef C,Kaneko T,Cecchini M,Han VK,Li SS

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Mon Oct 10 00:00:00 EDT 2022

Glial receptor PLXNB2 regulates schizophrenia-related stress perception via the amygdala.

Frontiers in immunology

Xuan FL,Yan L,Li Y,Fan F,Deng H,Gou M,Chithanathan K,Heinla I,Yuan L,Seppa K,Zharkovsky A,Kalda A,Hong LE,Hu GF,Tan Y,Tian L

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Mon Nov 07 00:00:00 EST 2022

Activated microglia mitigate Aβ-associated tau seeding and spreading.

The Journal of experimental medicine

Gratuze M,Chen Y,Parhizkar S,Jain N,Strickland MR,Serrano JR,Colonna M,Ulrich JD,Holtzman DM

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Mon Aug 02 00:00:00 EDT 2021

Regionally diverse astrocyte subtypes and their heterogeneous response to EAE.

Glia

Borggrewe M,Grit C,Vainchtein ID,Brouwer N,Wesseling EM,Laman JD,Eggen BJL,Kooistra SM,Boddeke EWGM

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Sat May 01 00:00:00 EDT 2021

CSF SERPINA3 Levels Are Elevated in Patients With Progressive MS.

Neurology(R) neuroimmunology & neuroinflammation

Fissolo N,Matute-Blanch C,Osman M,Costa C,Pinteac R,Miró B,Sanchez A,Brito V,Dujmovic I,Voortman M,Khalil M,Borràs E,Sabidó E,Issazadeh-Navikas S,Montalban X,Comabella Lopez M

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Mon Mar 01 00:00:00 EST 2021

CSF SERPINA3 Levels Are Elevated in Patients With Progressive MS.

Neurology(R) neuroimmunology & neuroinflammation

Fissolo N,Matute-Blanch C,Osman M,Costa C,Pinteac R,Miró B,Sanchez A,Brito V,Dujmovic I,Voortman M,Khalil M,Borràs E,Sabidó E,Issazadeh-Navikas S,Montalban X,Comabella Lopez M

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Mon Mar 01 00:00:00 EST 2021

Developmental Role of Adenosine Kinase in the Cerebellum.

eNeuro

Gebril H,Wahba A,Zhou X,Lai T,Alharfoush E,DiCicco-Bloom E,Boison D

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Fri Jul 02 00:00:00 EDT 2021

Studies of involvement of G-protein coupled receptor-3 in cannabidiol effects on inflammatory responses of mouse primary astrocytes and microglia.

PloS one

Wu J,Chen N,Liu Y,Godlewski G,Kaplan HJ,Shrader SH,Song ZH,Shao H

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Thu Oct 21 00:00:00 EDT 2021

Bidens pilosa Extract Administered after Symptom Onset Attenuates Glial Activation, Improves Motor Performance, and Prolongs Survival in a Mouse Model of Amyotrophic Lateral Sclerosis.

Oxidative medicine and cellular longevity

Kosuge Y,Kaneko E,Nango H,Miyagishi H,Ishige K,Ito Y

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunocytochemistry

Thu Sep 17 00:00:00 EDT 2020

Muscovite nanoparticles mitigate neuropathic pain by modulating the inflammatory response and neuroglial activation in the spinal cord.

Neural regeneration research

Oh JY,Hwang TY,Jang JH,Park JY,Ryu Y,Lee H,Park HJ

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry (Frozen)

Sun Nov 01 00:00:00 EDT 2020

Plasma Levels of Neuron- and Astrocyte-Derived Exosomal Amyloid Beta1-42, Amyloid Beta1-40, and Phosphorylated Tau Levels in Schizophrenia Patients and Non-psychiatric Comparison Subjects: Relationships With Cognitive Functioning and Psychopathology.

Frontiers in psychiatry

Lee EE,Winston-Gray C,Barlow JW,Rissman RA,Jeste DV

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Western Blot

Sat Mar 27 00:00:00 EDT 2021

Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer's disease.

Molecular neurodegeneration

Rayaprolu S,Gao T,Xiao H,Ramesha S,Weinstock LD,Shah J,Duong DM,Dammer EB,Webster JA,Lah JJ,Wood LB,Betarbet R,Levey AI,Seyfried NT,Rangaraju S

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Thu May 07 00:00:00 EDT 2020

Inhibition of GSK3β and RIP1K Attenuates Glial Scar Formation Induced by Ischemic Stroke via Reduction of Inflammatory Cytokine Production.

Frontiers in pharmacology

Liu J,Zhu YM,Guo Y,Lin L,Wang ZX,Gu F,Dong XY,Zhou M,Wang YF,Zhang HL

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Mon Sep 28 00:00:00 EDT 2020

Inhibition of GSK3β and RIP1K Attenuates Glial Scar Formation Induced by Ischemic Stroke via Reduction of Inflammatory Cytokine Production.

Frontiers in pharmacology

Liu J,Zhu YM,Guo Y,Lin L,Wang ZX,Gu F,Dong XY,Zhou M,Wang YF,Zhang HL

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Mon Sep 28 00:00:00 EDT 2020

Oxidative stress in the retina and retinal pigment epithelium (RPE): Role of aging, and DJ-1.

Redox biology

Upadhyay M,Milliner C,Bell BA,Bonilha VL

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Thu Oct 01 00:00:00 EDT 2020

Neuroprotective effect of ACTH on collagenase-induced peri-intraventricular hemorrhage in newborn male rats.

Scientific reports

Martins CA,Neves LT,de Oliveira MMBP,Bagatini PB,Barboza R,Mestriner RG,Xavier LL,Rasia-Filho AA

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunocytochemistry

Tue Oct 20 00:00:00 EDT 2020

FGL2 promotes tumor progression in the CNS by suppressing CD103⁺ dendritic cell differentiation.

Nature communications

Yan J,Zhao Q,Gabrusiewicz K,Kong LY,Xia X,Wang J,Ott M,Xu J,Davis RE,Huo L,Rao G,Sun SC,Watowich SS,Heimberger AB,Li S

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunofluorescence

Fri Jan 25 00:00:00 EST 2019

CHIMERA repetitive mild traumatic brain injury induces chronic behavioural and neuropathological phenotypes in wild-type and APP/PS1 mice.

Alzheimer's research & therapy

Cheng WH,Martens KM,Bashir A,Cheung H,Stukas S,Gibbs E,Namjoshi DR,Button EB,Wilkinson A,Barron CJ,Cashman NR,Cripton PA,Wellington CL

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunohistochemistry

Sat Jan 12 00:00:00 EST 2019

Roles of Pannexin-1 Channels in Inflammatory Response through the TLRs/NF-Kappa B Signaling Pathway Following Experimental Subarachnoid Hemorrhage in Rats.

Frontiers in molecular neuroscience

Wu LY,Ye ZN,Zhou CH,Wang CX,Xie GB,Zhang XS,Gao YY,Zhang ZH,Zhou ML,Zhuang Z,Liu JP,Hang CH,Shi JX

Published figure using GFAP monoclonal antibody (Product # 42-9892-82) in Immunofluorescence

Thu Oct 01 00:00:00 EDT 2020

Development of a culture system to induce microglia-like cells from haematopoietic cells.

Neuropathology and applied neurobiology

Noto D,Sakuma H,Takahashi K,Saika R,Saga R,Yamada M,Yamamura T,Miyake S

42-9892 was used in Immunohistochemistry to outline the conditions and factors necessary for the differentiation of microglia from progenitors.

Wed Oct 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein

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Gene Aliases: AI836096; ALXDRD; GFAP

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UniProt ID: (Human) E9PAX3, (Rat) P47819, (Mouse) P03995

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Entrez Gene ID: (Rabbit) 100352532, (Pig) 396562, (Chicken) 419969, (Human) 2670, (Rat) 24387, (Mouse) 14580

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Function(s)

glycoprotein binding integrin binding structural constituent of cytoskeleton protein binding kinase binding structural molecule activity

Process(es)

response to wounding positive regulation of Schwann cell proliferation negative regulation of neuron projection development astrocyte development extracellular matrix organization neuron projection regeneration intermediate filament-based process intermediate filament organization regulation of neurotransmitter uptake Bergmann glial cell differentiation positive regulation of glial cell proliferation long-term synaptic potentiation regulation of protein complex assembly regulation of chaperone-mediated autophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GFAP Monoclonal Antibody (GA5), eFluor™ 615, eBioscience™

GFAP Antibody (42-9892-82) in ICC/IF

Product Details

42-9892-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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