Invitrogen

GAP43 Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

GAP43 Antibody in Immunocytochemistry (ICC/IF)

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GAP43 Antibody (PA5-85949) in ICC/IF

Immunofluorescence analysis of GAP43 was performed using 70% confluent log phase SH-SY5Yneuronal differentiated and undifferentiated cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GAP43 Polyclonal Antibody (Product # PA5-85949) at 1:200 dilution in 0.1% BSA, incubated at 4-degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary... View More

GAP43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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GAP43 Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across the cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of GAP43 was observed in SH-SY5Y differentiated to neurons as compared to undifferentiated cells and in other brain-lineage cell lines and tissues but not in Mouse lung which is reported to be low to negative for GAP43 expression using Anti-GAP43 Polyclonal Antibody (Product # PA5-85949) in Western Blot. {RE}

Product Details

PA5-85949

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:200

Flow Cytometry (Flow)

1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within mouse GAP43 aa 178-227

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 40% Glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802750

Target Information

GAP43 (Neuromodulin) is associated with nerve growth. GAP43 is expressed by developing and regenerating neurons, and to a lesser extent reactive glial cells. It is a major component of the motile "growth cones" that form the tips of elongating axons. GAP43 also plays a role in axonal and dendritic filopodia induction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Axonal membrane protein GAP-43; brain abundant, membrane attached signal protein 2; Calmodulin-binding protein P-57; growth accentuating protein 43; Growth-associated protein 43; MGC156666; nerve growth-related peptide GAP43; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; neuron protein F1; pp46; Protein F1

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Gene Aliases: B-50; Basp2; GAP-43; GAP43; PP46

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UniProt ID: (Human) P17677, (Rat) P07936, (Mouse) P06837

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Entrez Gene ID: (Human) 2596, (Rat) 29423, (Mouse) 14432

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Function(s)

phosphatidylserine binding protein binding calmodulin binding lysophosphatidic acid binding phosphatidylinositol phosphate binding

Process(es)

nervous system development axon guidance glial cell differentiation axon choice point recognition regulation of growth tissue regeneration cell fate commitment regulation of filopodium assembly axon development multicellular organism development cell differentiation protein kinase C-activating G-protein coupled receptor signaling pathway response to wounding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GAP43 Polyclonal Antibody

GAP43 Antibody (PA5-85949) in ICC/IF

Product Details

PA5-85949

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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