Invitrogen

GABA Polyclonal Antibody

GABA Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

GABA Antibody (PA5-32241) in ICC/IF

Immunofluorescence analysis of GABA neurons using anti-GABA antibody (Product # PA5-32241). GABAergic precursor cells derived from H9 embryonic stem cells were differentiated for 7 days and stained with antibodies against GABA (Product # PA5-32241 at 1:5000) followed by Alexa Fluor 488 goat anti-rabbit (Product # A-21206, green) and neuronal marker beta III tubulin (Product # 480011 at 1:1000) followed by by Alexa Fluor 594 goat-anti-mouse (Product # A-11005, red). Nuclear DNA was stained with DAPI (blue).

GABA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GABA Antibody in Immunohistochemistry (Frozen) (IHC (F))

GABA Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA5-32241

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

Dot blot (DB)

Assay-Dependent

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GABA conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.71 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2549714

Product Specific Information

Recommended positive controls: GABA-conjugated BSA, mouse brain.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This chemical acts as a neurotransmitter in the central nervous system. It reduces neuronal excitation and regulates muscle tone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Genetic labeling reveals spatial and cellular expression pattern of neuregulin 1 in mouse brain.

Cell & bioscience

Ding CY,Ding YT,Ji H,Wang YY,Zhang X,Yin DM

PA5-32241 was used in Immunohistochemistry-immunofluorescence to reveal the cellular expression pattern of Nrg1 and axon projections of Nrg1-positive neurons in the rodent brain using CRISPR/Cas9 techniques, with the aim of understanding the diverse expression patterns of Neuregulin 1 (Nrg1) in different brain regions.

Fri May 05 00:00:00 EDT 2023

Pharmacotherapy alleviates pathological changes in human direct reprogrammed neuronal cell model of myotonic dystrophy type 1.

PloS one

Eltahir MK,Nakamori M,Hattori S,Kimura T,Mochizuki H,Nagano S

PA5-32241 was used in Immunocytochemistry to prove that our model is a promising tool for detailed pathogenetic examination and novel drug screening for the nervous system.

Thu Jul 07 00:00:00 EDT 2022

Raphe serotonin projections dynamically regulate feeding behavior through targeting inhibitory circuits from rostral zona incerta to paraventricular thalamus.

Molecular metabolism

Ye Q,Nunez J,Zhang X

PA5-32241 was used in Immunocytochemistry to reveal that raphe-ZIR 5-HT projections dynamically regulate ZIR GABA neurons for feeding control, supporting that a dynamic fluctuation of ZIR 5-HT inhibition authorizes daily food intake but a sustained change of ZIR 5-HT signaling leads to overeating induced by HFHS diet.

Thu Dec 01 00:00:00 EST 2022

Chronic loss of inhibition in piriform cortex following brief, daily optogenetic stimulation.

Cell reports

Ryu B,Nagappan S,Santos-Valencia F,Lee P,Rodriguez E,Lackie M,Takatoh J,Franks KM

PA5-32241 was used in Immunohistochemistry to find no loss of inhibitory interneurons; rather, decreased GABA synthesis in feedback inhibitory neurons appears to underlie weakened inhibition.

Tue Apr 20 00:00:00 EDT 2021

Chronic loss of inhibition in piriform cortex following brief, daily optogenetic stimulation.

Cell reports

Ryu B,Nagappan S,Santos-Valencia F,Lee P,Rodriguez E,Lackie M,Takatoh J,Franks KM

PA5-32241 was used in Immunohistochemistry to find no loss of inhibitory interneurons; rather, decreased GABA synthesis in feedback inhibitory neurons appears to underlie weakened inhibition.

Tue Apr 20 00:00:00 EDT 2021

Whole-Brain Monosynaptic Inputs to Hypoglossal Motor Neurons in Mice.

Neuroscience bulletin

Guo H,Yuan XS,Zhou JC,Chen H,Li SQ,Qu WM,Huang ZL

PA5-32241 was used in Immunohistochemistry to further elucidate the neural circuits underlying disorders caused by the dysfunction of hypoglossal motor neurons (HMNs).

Mon Jun 01 00:00:00 EDT 2020

Chemogenetic silencing of hippocampal neurons suppresses epileptic neural circuits.

The Journal of clinical investigation

Zhou QG,Nemes AD,Lee D,Ro EJ,Zhang J,Nowacki AS,Dymecki SM,Najm IM,Suh H

PA5-32241 was used in Immunohistochemistry to identify, using a mapping and neuronal manipulation method, newborn hippocampal dentate granule cells (DGCs) as an essential component of epileptic neural circuits.

Wed Jan 02 00:00:00 EST 2019

Bioinformatics

Protein Aliases: y-Aminobutyric acid

View more View less

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

GABA Polyclonal Antibody

GABA Antibody (PA5-32241) in ICC/IF

Product Details

PA5-32241

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Dot blot (DB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!