Product References
Chemical probe mediated visualization of protein S-palmitoylation in patient tissue samples.
Frontiers in physiology
Schek N,Lee JY,Burslem GM,Witze E
A-889 was used in Proximity Ligation Assay (PLA) to detect either areas enriched in palmitoylated proteins or the localization of specific palmitoylated proteins using our ABE-PLA methodology.
Fri May 19 00:00:00 EDT 2023
Pathways for Contextual Memory: The Primate Hippocampal Pathway to Anterior Cingulate Cortex.
Cerebral cortex (New York, N.Y. : 1991)
Wang J,John Y,Barbas H
A-889 was used in Immunohistochemistry to elucidate the organization of the pathway from the anterior hippocampus to anterior cingulate cortex so as to identify laminar termination patterns and their postsynaptic excitatory and inhibitory targets from system to synapse in rhesus monkeys.
Fri Feb 05 00:00:00 EST 2021
Tumor-penetrating therapy for β5 integrin-rich pancreas cancer.
Nature communications
Hurtado de Mendoza T,Mose ES,Botta GP,Braun GB,Kotamraju VR,French RP,Suzuki K,Miyamura N,Teesalu T,Ruoslahti E,Lowy AM,Sugahara KN
Published figure using Fluorescein/Oregon Green polyclonal antibody (Product # A-889) in Immunohistochemistry
Tue Mar 09 00:00:00 EST 2021
Tumor-penetrating therapy for β5 integrin-rich pancreas cancer.
Nature communications
Hurtado de Mendoza T,Mose ES,Botta GP,Braun GB,Kotamraju VR,French RP,Suzuki K,Miyamura N,Teesalu T,Ruoslahti E,Lowy AM,Sugahara KN
Published figure using Fluorescein/Oregon Green polyclonal antibody (Product # A-889) in Immunohistochemistry
Tue Mar 09 00:00:00 EST 2021
Spatial organization of transcribing loci during early genome activation in Drosophila.
Current biology : CB
Huang SK,Whitney PH,Dutta S,Shvartsman SY,Rushlow CA
A-889 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry to find a uniform distribution of distances between active genes in the nuclei of both WT and zld mutant embryos, indicating that early co-regulated genes do not cluster into nuclear sub-domains.
Mon Nov 22 00:00:00 EST 2021
Sensory Discrimination of Blood and Floral Nectar by Aedes aegypti Mosquitoes.
Neuron
Jové V,Gong Z,Hol FJH,Zhao Z,Sorrells TR,Carroll TS,Prakash M,McBride CS,Vosshall LB
A-889 was used in Immunohistochemistry to elucidate how Aedes aegypti female mosquitoes use specialized neurons to discriminate between blood and nectar.
Wed Dec 23 00:00:00 EST 2020
Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron.
Genes & development
Irizarry J,McGehee J,Kim G,Stein D,Stathopoulos A
A-889 was used in Immunohistochemistry to demonstrate that continuous input is not required for some Dorsal targets and downstream responses, such as twist, function as molecular ratchets.
Wed Jul 01 00:00:00 EDT 2020
Robustness of the Dorsal morphogen gradient with respect to morphogen dosage.
PLoS computational biology
Al Asafen H,Bandodkar PU,Carrell-Noel S,Schloop AE,Friedman J,Reeves GT
A-889 was used in Immunohistochemistry to highlight the need for quantitative understanding of biophysical mechanisms of morphogen gradients in order to understand emergent phenotypes, such as robustness.
Wed Apr 01 00:00:00 EDT 2020
Reliable measurement of free Ca2+ concentrations in the ER lumen using Mag-Fluo-4.
Cell calcium
Rossi AM,Taylor CW
A-889 was used in Flow Cytometry to show that partially de-esterified Mag-Fluo-4 has reduced affinity for Ca2+.
Fri May 01 00:00:00 EDT 2020
Hmx3a Has Essential Functions in Zebrafish Spinal Cord, Ear and Lateral Line Development.
Genetics
England SJ,Cerda GA,Kowalchuk A,Sorice T,Grieb G,Lewis KE
A-889 was used in Immunohistochemistry to show that zebrafish (Danio rerio) hmx2 and hmx3a are coexpressed in spinal dI2 and V1 interneurons, whereas hmx3b, hmx1, and hmx4 are not expressed in spinal cord.
Tue Dec 01 00:00:00 EST 2020
Fibroblast-specific Stat1 deletion enhances the myofibroblast phenotype during tissue repair.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
Medley SC,Rathnakar BH,Georgescu C,Wren JD,Olson LE
A-889 was used in Immunohistochemistry to suggest an in vivo role for Stat1 as a suppressor of fibroblast mediated, but not VSMC mediated, injury responses, and a suppressor of the myofibroblast phenotype.
Wed Jul 01 00:00:00 EDT 2020
Bi-specific tenascin-C and fibronectin targeted peptide for solid tumor delivery.
Biomaterials
Lingasamy P,Tobi A,Haugas M,Hunt H,Paiste P,Asser T,Rätsep T,Kotamraju VR,Bjerkvig R,Teesalu T
A-889 was used in Immunohistochemistry to identify a novel targeting peptide (PL1, sequence: PPRRGLIKLKTS) that interacts with both oncofetal fibronectin and the tenascin-C C domain using peptide phage biopanning.
Tue Oct 01 00:00:00 EDT 2019
Multi-enhancer transcriptional hubs confer phenotypic robustness.
eLife
Tsai A,Alves MR,Crocker J
A-889 was used in Immunohistochemistry to demonstrate a mechanism for phenotypical robustness by use of multiple enhancers which can reinforce a local transcriptional hub to buffer against environmental stresses and genetic pertubations.
Thu Jul 11 00:00:00 EDT 2019
Coacting enhancers can have complementary functions within gene regulatory networks and promote canalization.
PLoS genetics
Dunipace L,Ákos Z,Stathopoulos A
A-889 was used in Immunohistochemistry to show that similar gene expression driven by coacting enhancers can support distinct, and sometimes complementary, functions within gene regulatory networks and, moreover, that phenotypes associated with individual enhancer deletion mutants can provide insight into new gene functions.
Sun Dec 01 00:00:00 EST 2019
Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos.
Journal of visualized experiments : JoVE
Ferguson JL,Shive HR
A-889 was used in Immunohistochemistry to perform sequential immunofluorescence and/or immunohistochemistry on individual cryosections derived from early-stage zebrafish embryos.
Tue May 14 00:00:00 EDT 2019
Heat Shock Factor 5 Is Essential for Spermatogenesis in Zebrafish.
Cell reports
Saju JM,Hossain MS,Liew WC,Pradhan A,Thevasagayam NM,Tan LSE,Anand A,Olsson PE,Orbán L
A-889 was used in Immunohistochemistry-immunofluorescence to study a member of the heat shock factor family, Hsf5 using model organism Danio rerio.
Tue Dec 18 00:00:00 EST 2018
The WDR11 complex facilitates the tethering of AP-1-derived vesicles.
Nature communications
Navarro Negredo P,Edgar JR,Manna PT,Antrobus R,Robinson MS
A-889 was used in Immunocytochemistry to demonstrate that WDR11 forms a stable complex with two other proteins, which localises to the TGN region and does not appear to be associated with AP-1, suggesting it may act downstream from budding.
Fri Feb 09 00:00:00 EST 2018
Targeting of p32 in peritoneal carcinomatosis with intraperitoneal linTT1 peptide-guided pro-apoptotic nanoparticles.
Journal of controlled release : official journal of the Controlled Release Society
Hunt H,Simón-Gracia L,Tobi A,Kotamraju VR,Sharma S,Nigul M,Sugahara KN,Ruoslahti E,Teesalu T
A-889 was used in Immunohistochemistry-immunofluorescence to show that targeting of p32 with linTT1 tumor-penetrating peptide improves tumor selectivity and antitumor efficacy of IP pro-apoptotic NWs.
Mon Aug 28 00:00:00 EDT 2017
Targeting of p32 in peritoneal carcinomatosis with intraperitoneal linTT1 peptide-guided pro-apoptotic nanoparticles.
Journal of controlled release : official journal of the Controlled Release Society
Hunt H,Simón-Gracia L,Tobi A,Kotamraju VR,Sharma S,Nigul M,Sugahara KN,Ruoslahti E,Teesalu T
A-889 was used in Immunohistochemistry-immunofluorescence to show that targeting of p32 with linTT1 tumor-penetrating peptide improves tumor selectivity and antitumor efficacy of IP pro-apoptotic NWs.
Mon Aug 28 00:00:00 EDT 2017
Identification of a peptide recognizing cerebrovascular changes in mouse models of Alzheimer's disease.
Nature communications
Mann AP,Scodeller P,Hussain S,Braun GB,Mölder T,Toome K,Ambasudhan R,Teesalu T,Lipton SA,Ruoslahti E
A-889 was used in Immunohistochemistry-immunofluorescence to search for molecular markers of the neurovascular unit, including endothelial cells and astrocytes, in mouse models of AD.
Fri Nov 10 00:00:00 EST 2017
Tbx16 regulates hox gene activation in mesodermal progenitor cells.
Nature chemical biology
Payumo AY,McQuade LE,Walker WJ,Yamazoe S,Chen JK
A889 was used in immunohistochemistry - paraffin section to report the Tbx16 transcriptome in mesodermal progenitor cells
Thu Sep 01 00:00:00 EDT 2016
A peptide for targeted, systemic delivery of imaging and therapeutic compounds into acute brain injuries.
Nature communications
Mann AP,Scodeller P,Hussain S,Joo J,Kwon E,Braun GB,Mölder T,She ZG,Kotamraju VR,Ranscht B,Krajewski S,Teesalu T,Bhatia S,Sailor MJ,Ruoslahti E
Published figure using Fluorescein/Oregon Green polyclonal antibody (Product # A-889) in Immunofluorescence
Tue Jun 28 00:00:00 EDT 2016
Distribution of the sex chromosome during mouse spermatogenesis in testis tissue sections.
The Journal of reproduction and development
Otaka K,Hiradate Y,Kobayashi N,Shirakata Y,Tanemura K
A-889 was used in immunohistochemistry - paraffin section to study the distribution in testisi tissue sections of the sex chromosome during mouse spermatogenesis
Fri Aug 12 00:00:00 EDT 2016
Distribution of the sex chromosome during mouse spermatogenesis in testis tissue sections.
The Journal of reproduction and development
Otaka K,Hiradate Y,Kobayashi N,Shirakata Y,Tanemura K
A-889 was used in immunohistochemistry - paraffin section to study the distribution in testisi tissue sections of the sex chromosome during mouse spermatogenesis
Fri Aug 12 00:00:00 EDT 2016
A free cysteine prolongs the half-life of a homing peptide and improves its tumor-penetrating activity.
Journal of controlled release : official journal of the Controlled Release Society
Pang HB,Braun GB,She ZG,Kotamraju VR,Sugahara KN,Teesalu T,Ruoslahti E
Published figure using Fluorescein/Oregon Green polyclonal antibody (Product # A-889) in Western Blot
Mon Feb 10 00:00:00 EST 2014
Specialized pathways from the primate amygdala to posterior orbitofrontal cortex.
The Journal of neuroscience : the official journal of the Society for Neuroscience
Timbie C,Barbas H
A-889 was used in immunohistochemistry - free floating to investigate the interactions between primate amygdala and the posterior orbitofrontal cortex.
Wed Jun 11 00:00:00 EDT 2014
The cholinergic basal forebrain in the ferret and its inputs to the auditory cortex.
The European journal of neuroscience
Bajo VM,Leach ND,Cordery PM,Nodal FR,King AJ
Published figure using Fluorescein/Oregon Green polyclonal antibody (Product # A-889) in Immunohistochemistry
Mon Sep 01 00:00:00 EDT 2014
An endocytosis pathway initiated through neuropilin-1 and regulated by nutrient availability.
Nature communications
Pang HB,Braun GB,Friman T,Aza-Blanc P,Ruidiaz ME,Sugahara KN,Teesalu T,Ruoslahti E
Published figure using Fluorescein/Oregon Green polyclonal antibody (Product # A-889) in Immunofluorescence
Fri Oct 03 00:00:00 EDT 2014
Amygdala projections to the lateral bed nucleus of the stria terminalis in the macaque: comparison with ventral striatal afferents.
The Journal of comparative neurology
deCampo DM,Fudge JL
A-889 was used in immunohistochemistry to study the signal transmission between amygdala and the lateral bed nucleus of the stria terminalis in the macaque
Tue Oct 01 00:00:00 EDT 2013
Identification of enzyme-converted peptide products from single cells using capillary electrophoresis and liquid chromatography-mass spectrometry.
Methods in molecular biology (Clifton, N.J.)
Brown RB,Hewel JA,Emili A,Audet J
A-889 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, RNA Immunoprecipitation to study a method for determining the identity of enzyme-converted peptide products from single cells using a combination of capillary electrophoresis and liquid chromatography-mass spectrometry.
Tue May 29 00:00:00 EDT 2012
Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility.
International journal of biomaterials
Schleicher M,Hansmann J,Elkin B,Kluger PJ,Liebscher S,Huber AJ,Fritze O,Schille C,Müller M,Schenke-Layland K,Seifert M,Walles H,Wendel HP,Stock UA
A-889 was used in ELISA to describe a method to generate cell adhesive DNA-oligonucleotide coatings on vascular implant surfaces.
Thu Aug 23 00:00:00 EDT 2012
BH3 domains other than Bim and Bid can directly activate Bax/Bak.
The Journal of biological chemistry
Du H,Wolf J,Schafer B,Moldoveanu T,Chipuk JE,Kuwana T
A-889 was used in western blot to elucidate the roles of BH3 peptides in mitochondrial outer membrane permeabilization and apoptosis
Fri Jan 07 00:00:00 EST 2011
An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules.
Nucleic acids research
Kimoto M,Kawai R,Mitsui T,Yokoyama S,Hirao I
Published figure using Fluorescein/Oregon Green polyclonal antibody (Product # A-889) in ChIP assay
Sun Feb 01 00:00:00 EST 2009
An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules.
Nucleic acids research
Kimoto M,Kawai R,Mitsui T,Yokoyama S,Hirao I
Published figure using Fluorescein/Oregon Green polyclonal antibody (Product # A-889) in ChIP assay
Sun Feb 01 00:00:00 EST 2009
Assessing the sensitivity of commercially available fluorophores to the intracellular environment.
Analytical chemistry
Chen AK,Cheng Z,Behlke MA,Tsourkas A
A-889 was used in blocking or activating experiment to analyze commercially available fluorophores to the intracellular environment by assessing sensitivity
Wed Oct 01 00:00:00 EDT 2008
C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium.
Blood
Dentelli P,Rosso A,Balsamo A,Colmenares Benedetto S,Zeoli A,Pegoraro M,Camussi G,Pegoraro L,Brizzi MF
A-889 was used in immunohistochemistry to study recruitment of endothelial progenitor cells to inflamed endothelium by C-KIT that interacts with the membrane-bound ligand
Tue May 15 00:00:00 EDT 2007
Mechanochemical analysis of DNA gyrase using rotor bead tracking.
Nature
Gore J,Bryant Z,Stone MD,Nöllmann M,Cozzarelli NR,Bustamante C
A-889 was used in flow cytometry to characterize the activities of DNA gyrase
Thu Jan 05 00:00:00 EST 2006
The vascular endothelial cell mediates insulin transport into skeletal muscle.
American journal of physiology. Endocrinology and metabolism
Wang H,Liu Z,Li G,Barrett EJ
A-889 was used in immunohistochemistry - paraffin section to determine mediation of insulin transport into skeletal muscle via the vascular endothelial cell
Tue Aug 01 00:00:00 EDT 2006
CD133+ renal progenitor cells contribute to tumor angiogenesis.
The American journal of pathology
Bruno S,Bussolati B,Grange C,Collino F,Graziano ME,Ferrando U,Camussi G
A-889 was used in immunohistochemistry - paraffin section to study the contribution to tumor angiogenesis by CD133+ renal progenitor cells
Fri Dec 01 00:00:00 EST 2006
Development of homogeneous binding assays based on fluorescence resonance energy transfer between quantum dots and Alexa Fluor fluorophores.
Analytical biochemistry
Nikiforov TT,Beechem JM
A-889 was used to analyze new homogeneous binding assays based on fluorescence resonance energy transfer between Alexa Fluor fluorophores and quantum dots
Sun Oct 01 00:00:00 EDT 2006
Distinct chemokine triggers and in vivo migratory paths of fluorescein dye-labeled T Lymphocytes in acutely simian immunodeficiency virus SIVmac251-infected and uninfected macaques.
Journal of virology
Clay CC,Rodrigues DS,Harvey DJ,Leutenegger CM,Esser U
A-889 was used in immunohistochemistry - paraffin section to study in vivo migratory paths of fluorescein dye-labeled T lymphocytes and distinct chemokine triggers in acutely simian immunodeficiency virus SIVmac251-infected and uninfected macaques
Tue Nov 01 00:00:00 EST 2005
Development of a direct and sensitive detection method for DNA-binding proteins based on electrophoretic mobility shift assay and iodoacetamide derivative labeling.
Analytical biochemistry
Adachi Y,Chen W,Shang WH,Kamata T
A-889 was used in western blot to study the development of a sensitive and direct detection method for DNA-binding proteins based on iodoacetamide derivative labeling and elecrophoretic mobility shift assay
Fri Jul 15 00:00:00 EDT 2005
Cranial neural crest contributes to the bony skull vault in adult Xenopus laevis: insights from cell labeling studies.
Journal of experimental zoology. Part B, Molecular and developmental evolution
Gross JB,Hanken J
A-889 was used in immunohistochemistry - frozen section to clarify the developmental origin of the ossified skull in adult anurans
Tue Mar 15 00:00:00 EST 2005
Using bifunctional polymers presenting vancomycin and fluorescein groups to direct anti-fluorescein antibodies to self-assembled monolayers presenting d-alanine-d-alanine groups.
Journal of the American Chemical Society
Metallo SJ,Kane RS,Holmlin RE,Whitesides GM
A-889 was used to study the direction of anti-fluorescein antibodies to self-assembled monolayers presenting d-alanine-d-alanine groups using bifunctional polymers presenting vancomycin and fluorescein groups
Wed Apr 16 00:00:00 EDT 2003
Drosophila polypyrimidine-tract binding protein (PTB) functions specifically in the male germline.
The EMBO journal
Robida MD,Singh R
A-889 was used in immunohistochemistry to study the male-specific expression of polypyrimidine-tract binding protein.
Mon Jun 16 00:00:00 EDT 2003
Reactive oxygen species as essential mediators of cell adhesion: the oxidative inhibition of a FAK tyrosine phosphatase is required for cell adhesion.
The Journal of cell biology
Chiarugi P,Pani G,Giannoni E,Taddei L,Colavitti R,Raugei G,Symons M,Borrello S,Galeotti T,Ramponi G
A-889 was used in western blot to learn about essential mediators of cell adhesions known as reactive oxygen species and the oxidative inhibition of a FAK tyrosine phosphate is required for cell adhesion
Mon Jun 09 00:00:00 EDT 2003
Assembly and topography of the prepore complex in cholesterol-dependent cytolysins.
The Journal of biological chemistry
Heuck AP,Tweten RK,Johnson AE
A-889 was used in blocking or activating experiment to analyze cholesterol-dependent cytolysins and topography and assembly of the prepore complex
Fri Aug 15 00:00:00 EDT 2003
Synthetic compound libraries displayed on the surface of encoded bacteriophage.
Chemistry & biology
Woiwode TF,Haggerty JE,Katz R,Gallop MA,Barrett RW,Dower WJ,Cwirla SE
A-889 was used to display synthetic ompound libraries on the surface of encoded bacteriophage
Mon Sep 01 00:00:00 EDT 2003
Beta-catenin interacts with low-molecular-weight protein tyrosine phosphatase leading to cadherin-mediated cell-cell adhesion increase.
Cancer research
Taddei ML,Chiarugi P,Cirri P,Buricchi F,Fiaschi T,Giannoni E,Talini D,Cozzi G,Formigli L,Raugei G,Ramponi G
A-889 was used in western blot to study the progression to cadherin-mediated cell-cell adhesion increase by Beta-catenin interacting with low-molecular-weight protein tyrosine phosphatase
Fri Nov 15 00:00:00 EST 2002
Diffraction-based assay for detecting multiple analytes.
Analytical and bioanalytical chemistry
Goh B,Loo RW,McAloney RA,Goh MC
A-889 was used in ELISA to discuss the simultaneous detection of multiple analytes in solution.
Sun Sep 01 00:00:00 EDT 2002
Relationship of lipid rafts to transient confinement zones detected by single particle tracking.
Biophysical journal
Dietrich C,Yang B,Fujiwara T,Kusumi A,Jacobson K
A-889 was used in immunocytochemistry to utilize single particle tracking to detect the relationship of lipid rafts to transient confinement zones
Tue Jan 01 00:00:00 EST 2002
Mapping the type I collagen-binding site on pigment epithelium-derived factor. Implications for its antiangiogenic activity.
The Journal of biological chemistry
Meyer C,Notari L,Becerra SP
A-889 was used in western blot to investigate the binding of PEDF to collagen I
Fri Nov 22 00:00:00 EST 2002
Assembly of a fibronectin matrix by adherent platelets stimulated by lysophosphatidic acid and other agonists.
Blood
Olorundare OE,Peyruchaud O,Albrecht RM,Mosher DF
A-889 was used in immunocytochemistry to test if lysophosphatidic acid and sphingosine-1-phosphate stimulate binding and assembly of fluoresceinated fibronectin by adherent platelets
Sun Jul 01 00:00:00 EDT 2001
BMP4 plays a key role in left-right patterning in chick embryos by maintaining Sonic Hedgehog asymmetry.
Molecular cell
Monsoro-Burq A,Le Douarin NM
A-889 was used in immunohistochemistry to report that early BMP4 signaling coordinates left and right pathways in Hensen's node
Sun Apr 01 00:00:00 EST 2001
Binding of cross-linked glycosylphosphatidylinositol-anchored proteins to discrete actin-associated sites and cholesterol-dependent domains.
Biophysical journal
Suzuki K,Sheetz MP
A-889 was used in immunocytochemistry to study the binding of cross-linked glycosylphosphatidylinositol-anchored proteins to discrete cholesterol-dependent domains and actin-associated sites
Mon Oct 01 00:00:00 EDT 2001
Localization of cells of the prospective neural plate, heart and somites within the primitive streak and epiblast of avian embryos at intermediate primitive-streak stages.
Cells, tissues, organs
Lopez-Sanchez C,Garcia-Martinez V,Schoenwolf GC
A-889 was used in immunocytochemistry to describe a prospective fate map of the primitive streak and epiblast of the avian blastoderm at intermediate primitive-streak stages.
Fri Dec 07 00:00:00 EST 2001
Two independent regions of HIV-1 Nef are required for connection with the endocytic pathway through binding to the mu 1 chain of AP1 complex.
Traffic (Copenhagen, Denmark)
Erdtmann L,Janvier K,Raposo G,Craig HM,Benaroch P,Berlioz-Torrent C,Guatelli JC,Benarous R,Benichou S
A-889 was used in immunocytochemistry to investigate Nef-induced alterations of membrane trafficking
Wed Nov 01 00:00:00 EST 2000
Outer membrane monolayer domains from two-dimensional surface scanning resistance measurements.
Biophysical journal
Suzuki K,Sterba RE,Sheetz MP
A-889 was used to define outer membrane barriers
Sat Jul 01 00:00:00 EDT 2000
Transplantation chimeras. Use in analyzing mechanisms of avian development.
Methods in molecular biology (Clifton, N.J.)
Darnell DK,Schoenwolf GC
A-889 was used in Immunocytochemistry-immunofluorescence to show that transplantation chimeras can be made in many species and sometimes between species, are formed when tissue is grafted from one embryo (the donor) to another (the host) and permitted to incorporate.
Mon Jun 19 00:00:00 EDT 2000
Chimeric forms of furin and TGN38 are transported with the plasma membrane in the trans-Golgi network via distinct endosomal pathways.
The Journal of cell biology
Mallet WG,Maxfield FR
A-889 was used in immunocytochemistry to study the trafficking of furin and TGN38 in endosomes
Mon Jul 26 00:00:00 EDT 1999
Lipid domains of mycobacteria studied with fluorescent molecular probes.
Molecular microbiology
Christensen H,Garton NJ,Horobin RW,Minnikin DE,Barer MR
A-889 was used in immunocytochemistry to study the native organization of lipid domains in the mycobacterial envelope
Mon Mar 01 00:00:00 EST 1999
Microenvironment of the high affinity ATP-binding site of Na+/K+-ATPase is slightly acidic.
Biochemical and biophysical research communications
Linnertz H,Lanz E,Gregor M,Antolovic R,Krumscheid R,Obsil T,Slavik J,Kovarik Z,Schoner W,Amler E
A-889 was used to study the high-affinity ATP-binding site of Na+/K+-ATPase
Fri Jan 08 00:00:00 EST 1999
Redox regulation of cell signaling by selenocysteine in mammalian thioredoxin reductases.
The Journal of biological chemistry
Sun QA,Wu Y,Zappacosta F,Jeang KT,Lee BJ,Hatfield DL,Gladyshev VN
A-889 was used in western blot to identify the carboxyl-terminal selenocysteine of thioredoxin reductases as a cellular redox sensor
Fri Aug 27 00:00:00 EDT 1999
Direct pathway from early/recycling endosomes to the Golgi apparatus revealed through the study of shiga toxin B-fragment transport.
The Journal of cell biology
Mallard F,Antony C,Tenza D,Salamero J,Goud B,Johannes L
A-889 was used in blocking or activating experiment to hypothesize that Shiga toxin B-fragment is transported directly from early/recycling endosomes to the Golgi apparatus
Mon Nov 16 00:00:00 EST 1998
An endocytosed TGN38 chimeric protein is delivered to the TGN after trafficking through the endocytic recycling compartment in CHO cells.
The Journal of cell biology
Ghosh RN,Mallet WG,Soe TT,McGraw TE,Maxfield FR
A-889 was used in blocking or activating experiment to study TGN38 trafficking from the cell surface to the trans Golgi network
Mon Aug 24 00:00:00 EDT 1998
Purification of the Plasma Membrane Ca2+-ATPase from Radish Seedlings by Calmodulin-Agarose Affinity Chromatography.
Plant physiology
Bonza C,Carnelli A,Rasi-Caldogno F
A-889 was used in western blot to develop a method to purify Ca2+-ATPase from the plasma membrane of germinating radish seeds.
Sun Feb 01 00:00:00 EST 1998
De novo induction of the organizer and formation of the primitive streak in an experimental model of notochord reconstitution in avian embryos.
Development (Cambridge, England)
Yuan S,Schoenwolf GC
A-889 was used in immunohistochemistry to develop a model system for analyzing reconstitution of the notochord using cultured blastoderm isolates lacking Hensen's node and the primitive streak.
Thu Jan 01 00:00:00 EST 1998
CD14-dependent internalization of bacterial lipopolysaccharide (LPS) is strongly influenced by LPS aggregation but not by cellular responses to LPS.
Journal of immunology (Baltimore, Md. : 1950)
Kitchens RL,Munford RS
A-889 was used in blocking or activating experiment and flow cytometry to investigate the impact of ligand aggregation and LPS-induced signaling on CD14-dependent LPS internalization kinetics in human monocytic THP-1 cells and murine macrophages
Sun Feb 15 00:00:00 EST 1998
Probing cellular protein targets of H2O2 with fluorescein-conjugated iodoacetamide and antibodies to fluorescein.
FEBS letters
Wu Y,Kwon KS,Rhee SG
A-889 was used in western blot to develop an assay to identify H2O2-sensitive proteins including protein tyrosine phosphatase 1B
Fri Nov 27 00:00:00 EST 1998
The ELF-97 alkaline phosphatase substrate provides a bright, photostable, fluorescent signal amplification method for FISH.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Paragas VB,Zhang YZ,Haugland RP,Singer VL
A-889 was used in immunocytochemistry to characterize the use of ELF-97 (2-(5'-chloro-2-phosphoryloxyphenyl)-6-chloro-4(3H)-quinazolinone) as a probe
Sat Mar 01 00:00:00 EST 1997
Uptake and incorporation of an epitope-tagged sialic acid donor into intact rat liver Golgi compartments. Functional localization of sialyltransferase overlaps with beta-galactosyltransferase but not with sialic acid O-acetyltransferase.
Molecular biology of the cell
Chammas R,McCaffery JM,Klein A,Ito Y,Saucan L,Palade G,Farquhar MG,Varki A
A-889 was used in ELISA, immunoprecipitation, and western blot to report that 9-O-acetylated sialoglycoproteins are preferentially segregated into a subset of vesicular carriers that concentrate membrane-bound, but not secretory, proteins
Fri Nov 01 00:00:00 EST 1996
Luminal pH regulated calcium release kinetics in sarcoplasmic reticulum vesicles.
Biochemistry
Donoso P,Beltrán M,Hidalgo C
A-889 was used to identify factors that influence calcium binding to triads isolated from rabbit skeletal muscle
Tue Oct 15 00:00:00 EDT 1996
Improving the efficacy of fluorescent labeling for histological tracking of cells in early mammalian and avian embryos.
The Anatomical record
Garton HJ,Schoenwolf GC
A-889 was used in immunohistochemistry to discuss methods to label living cells in early embryos
Mon Jan 01 00:00:00 EST 1996
Novel flow cytometric analysis of the progress and route of internalization of a monoclonal anti-carcinoembryonic antigen (CEA) antibody.
Cytometry
Ford CH,Tsaltas GC,Osborne PA,Addetia K
A-889 was used in flow cytometry to investigate the internalization of carcinoembryonic antigen
Fri Mar 01 00:00:00 EST 1996
Detection of plasma membrane Ca(2+)-ATPase activity in mouse T lymphocytes by flow cytometry using fluo-3-loaded vesicles.
Cytometry
Telford WG,Miller RA
A-889 was used in flow cytometry to develop and characterize a flow cytometric method to measure plasma membrane Ca(2+)-ATPase
Mon Jul 01 00:00:00 EDT 1996
Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.
Biochemistry
Driessen AJ,van den Hooven HW,Kuiper W,van de Kamp M,Sahl HG,Konings RN,Konings WN
A-889 was used in blocking or activating experiment to study nisin-induced permeabilize liposomes
Tue Feb 07 00:00:00 EST 1995
Immunochemical analysis shows all three domains of diphtheria toxin penetrate across model membranes.
The Journal of biological chemistry
Tortorella D,Sesardic D,Dawes CS,London E
A-889 was used to study membrane insertion of diphtheria toxin
Fri Nov 17 00:00:00 EST 1995
Interaction of [fluorescein-Trp25]glucagon with the human glucagon receptor expressed in Drosophila Schneider 2 cells.
The Journal of biological chemistry
Tota MR,Xu L,Sirotina A,Strader CD,Graziano MP
A-889 was used in blocking or activating experiment to characterize Drosophila Schneider 2 cells that express human glucagon receptor
Fri Nov 03 00:00:00 EST 1995
Characterization of a fluorescent substance P analog.
Biochemistry
Tota MR,Daniel S,Sirotina A,Mazina KE,Fong TM,Longmore J,Strader CD
A-889 was used in blocking or activating experiment to develop and characterize substance P labeled at Lys3 with fluorescein as a fluorescent probe for the neurokinin 1 receptor
Tue Nov 08 00:00:00 EST 1994
Reconstitution of membrane fusion sites. A total internal reflection fluorescence microscopy study of influenza hemagglutinin-mediated membrane fusion.
The Journal of biological chemistry
Hinterdorfer P,Baber G,Tamm LK
A-889 was used in blocking or activating experiment to develop methods for the reconstitution of membrane fusion sites of influenza hemagglutinin in a planar supported membrane system
Fri Aug 12 00:00:00 EDT 1994
Resonance energy transfer between guanine nucleotide binding protein subunits and membrane lipids.
Biochemistry
Remmers AE,Neubig RR
A-889 was used in blocking or activating experiment to measure the distances between sites on G protein subunits alpha, beta, and gamma and the lipid bilayer
Tue Mar 09 00:00:00 EST 1993
F-actin in acrosome-reacted boar spermatozoa.
Molecular reproduction and development
Castellani-Ceresa L,Brivio MF,Radaelli G
A-889 was used in immunohistochemistry to analyze the distribution of actin in boar spermatozoa using biochemical and microscopy methods.
Tue Sep 01 00:00:00 EDT 1992
Liposome-encapsulated antigens are processed in lysosomes, recycled, and presented to T cells.
Cell
Harding CV,Collins DS,Slot JW,Geuze HJ,Unanue ER
A-889 was used in immunocytochemistry to provide evidence that dense lysosomes produce immunogenic peptides that bind to class II MHC
Fri Jan 25 00:00:00 EST 1991
Modulation of intracellular pH by secretagogues and the Na+/H+ antiporter in cultured bovine chromaffin cells.
Neuroscience
Rosario LM,Stutzin A,Cragoe EJ,Pollard HB
A-889 was used in immunocytochemistry to examine the physiological role of cytosolic pH changes in adrenal medullary chromaffin cell secretion
Mon Jul 29 00:00:00 EDT 1991
Functional water channels and proton pumps are in separate populations of endocytic vesicles in toad bladder granular cells.
Biochemistry
Wang YX,Shi LB,Verkman AS
A-889 was used in blocking or activating experiment to determine if endocytic vesicles in toad bladder granular cells that contain the vasopressin-sensitive water channel fuse with acidic vesicles for entry into a lysosomal pathway
Tue Mar 19 00:00:00 EST 1991
Determinants of the phagosomal pH in macrophages. In situ assessment of vacuolar H(+)-ATPase activity, counterion conductance, and H+ "leak".
The Journal of biological chemistry
Lukacs GL,Rotstein OD,Grinstein S
A-889 was used in blocking or activating experiment to elucidate factors that determine the intraphagosomal pH in elicited murine peritoneal macrophages
Wed Dec 25 00:00:00 EST 1991
Regulation of free cytosolic Ca2+ concentration in the outer segments of bovine retinal rods by Na-Ca-K exchange measured with fluo-3. I. Efficiency of transport and interactions between cations.
The Journal of biological chemistry
Schnetkamp PP,Li XB,Basu DK,Szerencsei RT
A-889 was used in blocking or activating experiment to evaluate the regulation of free cytosolic Ca2+ concentration in bovine retina rod outer segments
Thu Dec 05 00:00:00 EST 1991
Comparative behavior of membrane protein-antibody complexes on motile fibroblasts: implications for a mechanism of capping.
The Journal of cell biology
Holifield BF,Ishihara A,Jacobson K
A-889 was used in immunocytochemistry to test aspects of the retrograde lipid flow hypothesis and provide evidence supporting a cytoskeletal-based mechanism for driving capping
Sat Dec 01 00:00:00 EST 1990
Co-assembly of plasma and cellular fibronectins into fibrils in human fibroblast cultures.
The Journal of cell biology
Peters DM,Portz LM,Fullenwider J,Mosher DF
A-889 was used in immunocytochemistry to describe fibril formation in human fibroblast cultures via co-assembly of plasma and cellular fibronectins
Sun Jul 01 00:00:00 EDT 1990
Endocytic vesicles from renal papilla which retrieve the vasopressin-sensitive water channel do not contain a functional H+ ATPase.
The Journal of cell biology
Lencer WI,Verkman AS,Arnaout MA,Ausiello DA,Brown D
A-889 was used in blocking or activating experiment to assess whether water channels pass through an acidic endosomal compartment of principal kidney collecting duct epithelial cells
Wed Aug 01 00:00:00 EDT 1990
Fluorescence analysis of the size of a binding pocket of a peptide receptor at natural abundance.
Biochemistry
Sklar LA,Fay SP,Seligmann BE,Freer RJ,Muthukumaraswamy N,Mueller H
A-889 was used to assess the topography of interaction of fluorescent formyl peptides with their receptor
Tue Jan 16 00:00:00 EST 1990
Phagosomal acidification is mediated by a vacuolar-type H(+)-ATPase in murine macrophages.
The Journal of biological chemistry
Lukacs GL,Rotstein OD,Grinstein S
A-889 was used in immunocytochemistry to elucidate the mechanism that regulates phagosomal acidification
Wed Dec 05 00:00:00 EST 1990
Fusion accessibility of endocytic compartments along the recycling and lysosomal endocytic pathways in intact cells.
The Journal of cell biology
Salzman NH,Maxfield FR
A-889 was used to identify the site of alpha2-macroglobulin accumulation as a prelysosomal late endosome
Wed Nov 01 00:00:00 EST 1989
Mapping myosin light chains by immunoelectron microscopy. Use of anti-fluorescyl antibodies as structural probes.
The Journal of cell biology
Katoh T,Lowey S
A-889 was used in immunocytochemistry to determine the location of the two classes of light chains in vertebrate fast muscle myosin within the myosin head
Sun Oct 01 00:00:00 EDT 1989
Probing the nucleotide binding site of sarcoplasmic reticulum (Ca2(+)-Mg2)-ATPase with anti-fluorescein antibodies.
Biochemical Society transactions
Mata AM,Schofield AE,Woodbine J,Lee AG,East JM
A-889 was used in ELISA and blocking or activating experiment to probe the nucleotide binding site of sarcoplasmic reticulum-ATPase using anti-fluorescein antibodies
Fri Dec 01 00:00:00 EST 1989
Probing the nucleotide-binding site of sarcoplasmic reticulum (Ca2+-Mg2+)-ATPase with anti-fluorescein antibodies.
FEBS letters
Mata AM,Lee AG,East JM
A-889 was used in ELISA to compare detection of native and denatured FITC-ATPase using ELISA
Mon Aug 14 00:00:00 EDT 1989
Multiple immunoenzyme staining techniques. Use of fluoresceinated, biotinylated and unlabelled monoclonal antibodies.
Journal of immunological methods
Van der Loos CM,Das PK,Van den Oord JJ,Houthoff HJ
A-889 was used in immunohistochemistry - frozen section to develop reagents for multistep double and triple immunoenzymatic methods
Wed Feb 08 00:00:00 EST 1989
An immunocytochemical method for the detection of fluorochrome-labelled DNA probes hybridized in situ with cellular RNA.
Journal of immunological methods
Serke S,Pachmann K
A-889 was used in immunocytochemistry to discuss detection systems for in situ hybridization of nucleic acids
Tue Sep 13 00:00:00 EDT 1988
Production and ELISA application of bispecific monoclonal antibodies against fluorescein isothiocyanate (FITC) and horseradish peroxidase (HRP).
Journal of immunological methods
Karawajew L,Behrsing O,Kaiser G,Micheel B
A-889 was used in ELISA to develop and characterize bispecific anti-FITC and anti-HRP antibodies as a detection system in EIA
Tue Jun 28 00:00:00 EDT 1988
Synexin-mediated fusion of bovine chromaffin granule ghosts. Effect of pH.
Biochimica et biophysica acta
Stutzin A,Cabantchik ZI,Lelkes PI,Pollard HB
A-889 was used in blocking or activating experiment to elucidate the effects of pH and calcium on synexin-mediated fusion of granules ghosts
Fri Nov 27 00:00:00 EST 1987
A fluorescence assay for monitoring and analyzing fusion biological membrane vesicles in vitro.
FEBS letters
Stutzin A
A-889 was used to develop and characterize a new method for examining the fusion of biological membrane vesicles
Mon Mar 03 00:00:00 EST 1986
Antigen valence determines the binding of nominal antigen to cytolytic T cell clones.
The Journal of experimental medicine
Siliciano RF,Colello RM,Keegan AD,Dintzis RZ,Dintzis HM,Shin HS
A-889 was used in flow cytometry to propose that a substantial degree of multivalence is needed to obtain stable binding of the nominal antigen FL to T cell clones
Thu Aug 01 00:00:00 EDT 1985
Charge transfer between fluorescein and tryptophan as a possible interaction in the binding of fluorescein to anti-fluorescein antibody.
Molecular immunology
Gudgin Templeton EF,Ware WR
A-889 was used to investigate fluorescein and 9-hydroxyphenylfluoron bound to high-affinity rabbit anti-fluorescein IgG antibody
Tue Jan 01 00:00:00 EST 1985
Oriented reconstitution of red cell membrane proteins and assessment of their transmembrane disposition by immunoquenching of fluorescence.
Biochimica et biophysica acta
Darmon A,Bar-Noy S,Ginsburg H,Cabantchik ZI
A-889 was used in blocking or activating experiment to develop methods for the oriented reconstitution of proteins and then evaluate their transmembrane disposition
Thu Jul 25 00:00:00 EDT 1985
Location and dynamics of a membrane-bound fluorescent hapten. A spectroscopic study.
Biochimica et biophysica acta
Stanton SG,Kantor AB,Petrossian A,Owicki JC
A-889 was used to study membrane-bound hapten
Wed Oct 03 00:00:00 EDT 1984
The dynamics of ligand-receptor interactions. Real-time analyses of association, dissociation, and internalization of an N-formyl peptide and its receptors on the human neutrophil.
The Journal of biological chemistry
Sklar LA,Finney DA,Oades ZG,Jesaitis AJ,Painter RG,Cochrane CG
A-889 was used in flow cytometry to investigate the interaction of a fluoresceinated N-formyl hexapeptide with its receptors on human neutrophils
Thu May 10 00:00:00 EDT 1984
Receptor-mediated endocytosis of transferrin and the uptake of fe in K562 cells: identification of a nonlysosomal acidic compartment.
Proceedings of the National Academy of Sciences of the United States of America
van Renswoude J,Bridges KR,Harford JB,Klausner RD
A-889 was used in immunocytochemistry to investigate the up take and transport of Fe-carrier transferrin
Fri Oct 01 00:00:00 EDT 1982
Analysis of ligand-receptor interactions with the fluorescence activated cell sorter.
Cytometry
Sklar LA,Finney DA
A-889 was used in flow cytometry to characterize the binding of a fluorescent peptide to human neutrophils by flow cytometry
Mon Nov 01 00:00:00 EST 1982
