Invitrogen

FITC Monoclonal Antibody (NAWESLEE), Biotin, eBioscience™

FIGURE: 1 / 1

FITC Antibody (13-7691-82) in Flow

Normal human peripheral blood cells were first stained with Anti-Human CD3 FITC (Product # 11-0037-42), and then 0.06 µg of Mouse IgG2a K Isotype Control Biotin (Product # 13-4724-85) (left) or 0.06 µg of Anti-FITC Biotin (right), followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

13-7691-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

NAWESLEE

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572805

Product Specific Information

Description: This NAWESLEE monoclonal antibody reacts to fluorescein isothyocianate (FITC), a derivative of fluorescein commonly used in flow cytometry and fluorescent microscopy. FITC-9 can be used for the separation of cells labeled with FITC-conjugated antibodies or for staining. Please note that in some instances the FITC signal can be quenched by the anti-FITC antibody; therefore, this phenomenon should be evaluated empirically.

Applications Reported: This NAWESLEE antibody has been reported for use in flow cytometric analysis.

Applications Tested: This NAWESLEE antibody has been tested by flow cytometric analysis of cells stained with FITC-conjugated antibody. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Urinary detection of early responses to checkpoint blockade and of resistance to it via protease-cleaved antibody-conjugated sensors.

Nature biomedical engineering

Mac QD,Sivakumar A,Phuengkham H,Xu C,Bowen JR,Su FY,Stentz SZ,Sim H,Harris AM,Li TT,Qiu P,Kwong GA

13-7691-82 was used in flow cytometry to show that random forest classifiers trained on mass spectrometry signatures from a library of αPD1-conjugated mass-barcoded activity sensors for differentially expressed tumour proteases and immune proteases can be used to detect early antitumour responses and discriminate resistance to ICB therapy driven by loss-of-function mutations in either the B2m or Jak1 genes.

Tue Mar 01 00:00:00 EST 2022

Bioinformatics

Protein Aliases: Fluorescein

View more View less

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more