Invitrogen

Endomucin Monoclonal Antibody (eBioV.7C7 (V.7C7)), eFluor™ 660, eBioscience™

Endomucin Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 49

Endomucin Antibody (50-5851-82) in Flow

Staining of bEnd-3 cells with 0.5 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (blue histogram) or 0.5 µg of Anti-Mouse Endomucin eFluor® 660 (purple histogram). Total viable cells were used for analysis.

Endomucin Antibody in Immunohistochemistry (IHC)

Endomucin Antibody in Immunocytochemistry (ICC/IF)

Endomucin Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Endomucin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

50-5851-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 22 publications 22 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 5 publications 5 publications

Flow Cytometry (Flow)

1 µg/test

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioV.7C7 (V.7C7)

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11220465

Product Specific Information

Description: The eBioV.7C7 monoclonal antibody reacts with mouse endomucin, which was identified in a search for cell-surface expressed endothelial cell markers. Endomucin is a 75 kDa type I integral membrane protein, with similarities to the sialomucin family of proteins including extensive O-linked glycosylation. Endomucin is expressed on endothelial cells, however, an exception is the high endothelial venules (HEV) of secondary lymphoid organs. In addition, it has been demonstrated that endomucin is expressed on CD34-c-Kit+Sca-1+Lin- hematopoietic progenitors, and that these cells are capable of multi-lineage long-term reconstitution of the hematopoietic compartment.

Applications Reported: This eBioV.7C7 (V.7C7) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioV.7C7 (V.7C7) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Endomucin (EMCN) also known as endothelial sialomucin or mucin-like sialoglycoprotein interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

Association of Differentially Altered Liver Fibrosis with Deposition of TGFBi in Stabilin-Deficient Mice.

International journal of molecular sciences

Krzistetzko J,Géraud C,Dormann C,Riedel A,Leibing T

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Fri Jun 30 00:00:00 EDT 2023

The VEGF Inhibitor Soluble Fms-like Tyrosine Kinase 1 Does Not Promote AKI-to-CKD Transition.

International journal of molecular sciences

van Aanhold CCL,Koudijs A,Dijkstra KL,Wolterbeek R,Bruijn JA,van Kooten C,Baelde HJ

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Fri Aug 26 00:00:00 EDT 2022

Angiogenic and molecular diversity determine hepatic melanoma metastasis and response to anti-angiogenic treatment.

Journal of translational medicine

Wohlfeil SA,Häfele V,Dietsch B,Weller C,Sticht C,Jauch AS,Winkler M,Schmid CD,Irkens AL,Olsavszky A,Schledzewski K,Reiners-Koch PS,Goerdt S,Géraud C

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Wed Feb 02 00:00:00 EST 2022

G-CSF Receptor Deletion Amplifies Cortical Bone Dysfunction in Mice With STAT3 Hyperactivation in Osteocytes.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Isojima T,Walker EC,Poulton IJ,McGregor NE,Wicks IP,Gooi JH,Martin TJ,Sims NA

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Sat Oct 01 00:00:00 EDT 2022

TFPI2 Promotes Perivascular Migration in an Angiotropism Model of Melanoma.

Frontiers in oncology

Mo J,Zhao X,Wang W,Zhao N,Dong X,Zhang Y,Cheng R,Sun B

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Sun Apr 24 00:00:00 EDT 2022

Indispensable role of Galectin-3 in promoting quiescence of hematopoietic stem cells.

Nature communications

Jia W,Kong L,Kidoya H,Naito H,Muramatsu F,Hayashi Y,Hsieh HY,Yamakawa D,Hsu DK,Liu FT,Takakura N

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Fri Apr 09 00:00:00 EDT 2021

Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation.

Nature communications

Heil J,Olsavszky V,Busch K,Klapproth K,de la Torre C,Sticht C,Sandorski K,Hoffmann J,Schönhaber H,Zierow J,Winkler M,Schmid CD,Staniczek T,Daniels DE,Frayne J,Metzgeroth G,Nowak D,Schneider S,Neumaier M,Weyer V,Groden C,Gröne HJ,Richter K,Mogler C,Taketo MM,Schledzewski K,Géraud C,Goerdt S,Koch PS

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunocytochemistry

Mon Nov 29 00:00:00 EST 2021

Imbalanced Activation of Wnt-/β-Catenin-Signaling in Liver Endothelium Alters Normal Sinusoidal Differentiation.

Frontiers in physiology

Koch PS,Sandorski K,Heil J,Schmid CD,Kürschner SW,Hoffmann J,Winkler M,Staniczek T,de la Torre C,Sticht C,Schledzewski K,Taketo MM,Trogisch FA,Heineke J,Géraud C,Goerdt S,Olsavszky V

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunocytochemistry

Fri Oct 22 00:00:00 EDT 2021

Post-myocardial infarction heart failure dysregulates the bone vascular niche.

Nature communications

Hoffmann J,Luxán G,Abplanalp WT,Glaser SF,Rasper T,Fischer A,Muhly-Reinholz M,Potente M,Assmus B,John D,Zeiher AM,Dimmeler S

50-5851-82 was used in Flow Cytometry to study the bone vascular niche in mice with post-myocardial infarction heart failure.

Fri Jun 25 00:00:00 EDT 2021

Post-myocardial infarction heart failure dysregulates the bone vascular niche.

Nature communications

Hoffmann J,Luxán G,Abplanalp WT,Glaser SF,Rasper T,Fischer A,Muhly-Reinholz M,Potente M,Assmus B,John D,Zeiher AM,Dimmeler S

50-5851-82 was used in Flow Cytometry to study the bone vascular niche in mice with post-myocardial infarction heart failure.

Fri Jun 25 00:00:00 EDT 2021

Lymphoangiocrine signals promote cardiac growth and repair.

Nature

Liu X,De la Cruz E,Gu X,Balint L,Oxendine-Burns M,Terrones T,Ma W,Kuo HH,Lantz C,Bansal T,Thorp E,Burridge P,Jakus Z,Herz J,Cleaver O,Torres M,Oliver G

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Tue Dec 01 00:00:00 EST 2020

Cancer-associated fibroblast-derived WNT2 increases tumor angiogenesis in colon cancer.

Angiogenesis

Unterleuthner D,Neuhold P,Schwarz K,Janker L,Neuditschko B,Nivarthi H,Crncec I,Kramer N,Unger C,Hengstschläger M,Eferl R,Moriggl R,Sommergruber W,Gerner C,Dolznig H

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Fri May 01 00:00:00 EDT 2020

YAP and TAZ maintain PROX1 expression in the developing lymphatic and lymphovenous valves in response to VEGF-C signaling.

Development (Cambridge, England)

Cha B,Ho YC,Geng X,Mahamud MR,Chen L,Kim Y,Choi D,Kim TH,Randolph GJ,Cao X,Chen H,Srinivasan RS

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Sun Dec 13 00:00:00 EST 2020

A New Gorilla Adenoviral Vector with Natural Lung Tropism Avoids Liver Toxicity and Is Amenable to Capsid Engineering and Vector Retargeting.

Journal of virology

Lu ZH,Dmitriev IP,Brough DE,Kashentseva EA,Li J,Curiel DT

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Mon May 04 00:00:00 EDT 2020

Vascular dimorphism ensured by regulated proteoglycan dynamics favors rapid umbilical artery closure at birth.

eLife

Nandadasa S,Szafron JM,Pathak V,Murtada SI,Kraft CM,O'Donnell A,Norvik C,Hughes C,Caterson B,Domowicz MS,Schwartz NB,Tran-Lundmark K,Veigl M,Sedwick D,Philipson EH,Humphrey JD,Apte SS

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunocytochemistry

Thu Sep 10 00:00:00 EDT 2020

Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.

Science advances

Li X,Wang L,Huang B,Gu Y,Luo Y,Zhi X,Hu Y,Zhang H,Gu Z,Cui J,Cao L,Guo J,Wang Y,Zhou Q,Jiang H,Fang C,Weng W,Chen X,Chen X,Su J

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.

Science advances

Li X,Wang L,Huang B,Gu Y,Luo Y,Zhi X,Hu Y,Zhang H,Gu Z,Cui J,Cao L,Guo J,Wang Y,Zhou Q,Jiang H,Fang C,Weng W,Chen X,Chen X,Su J

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Vascular endothelial growth factor pathway promotes osseointegration and CD31^hiEMCN^hi endothelium expansion in a mouse tibial implant model: an animal study.

The bone & joint journal

Ji G,Xu R,Niu Y,Li N,Ivashkiv L,Bostrom MPG,Greenblatt MB,Yang X

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2019

Vascular endothelial growth factor pathway promotes osseointegration and CD31^hiEMCN^hi endothelium expansion in a mouse tibial implant model: an animal study.

The bone & joint journal

Ji G,Xu R,Niu Y,Li N,Ivashkiv L,Bostrom MPG,Greenblatt MB,Yang X

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2019

Prenatal inflammation impairs intestinal microvascular development through a TNF-dependent mechanism and predisposes newborn mice to necrotizing enterocolitis.

American journal of physiology. Gastrointestinal and liver physiology

Yan X,Managlia E,Tan XD,De Plaen IG

50-5851-82 was used In vivo to provide new evidence that maternal inflammation decreases neonatal intestinal VEGF receptor 2 signaling and endothelial cell proliferation, impairs intestinal microvascular development, and predisposes neonatal mouse pups to necrotizing enterocolitis (NEC) through inflammatory cytokines such as TNF.

Mon Jul 01 00:00:00 EDT 2019

Primary cilia deficiency in neural crest cells models anterior segment dysgenesis in mouse.

eLife

Portal C,Rompolas P,Lwigale P,Iomini C

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunocytochemistry

Tue Dec 17 00:00:00 EST 2019

Aged marrow macrophages expand platelet-biased hematopoietic stem cells via Interleukin1B.

JCI insight

Frisch BJ,Hoffman CM,Latchney SE,LaMere MW,Myers J,Ashton J,Li AJ,Saunders J,Palis J,Perkins AS,McCabe A,Smith JN,McGrath KE,Rivera-Escalera F,McDavid A,Liesveld JL,Korshunov VA,Elliott MR,MacNamara KC,Becker MW,Calvi LM

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Thu Apr 18 00:00:00 EDT 2019

Targeting skeletal endothelium to ameliorate bone loss.

Nature medicine

Xu R,Yallowitz A,Qin A,Wu Z,Shin DY,Kim JM,Debnath S,Ji G,Bostrom MP,Yang X,Zhang C,Dong H,Kermani P,Lalani S,Li N,Liu Y,Poulos MG,Wach A,Zhang Y,Inoue K,Di Lorenzo A,Zhao B,Butler JM,Shim JH,Glimcher LH,Greenblatt MB

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Inhibition of Endosteal Vascular Niche Remodeling Rescues Hematopoietic Stem Cell Loss in AML.

Cell stem cell

Duarte D,Hawkins ED,Akinduro O,Ang H,De Filippo K,Kong IY,Haltalli M,Ruivo N,Straszkowski L,Vervoort SJ,McLean C,Weber TS,Khorshed R,Pirillo C,Wei A,Ramasamy SK,Kusumbe AP,Duffy K,Adams RH,Purton LE,Carlin LM,Lo Celso C

50-5851 was used in Flow cytometry to suggest that preventing degradation of the endosteal vasculature may improve current paradigms for treating acute myeloid leukemia.

Thu Jan 04 00:00:00 EST 2018

Regional astrocyte IFN signaling restricts pathogenesis during neurotropic viral infection.

The Journal of clinical investigation

Daniels BP,Jujjavarapu H,Durrant DM,Williams JL,Green RR,White JP,Lazear HM,Gale M,Diamond MS,Klein RS

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Wed Mar 01 00:00:00 EST 2017

Regional astrocyte IFN signaling restricts pathogenesis during neurotropic viral infection.

The Journal of clinical investigation

Daniels BP,Jujjavarapu H,Durrant DM,Williams JL,Green RR,White JP,Lazear HM,Gale M,Diamond MS,Klein RS

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Wed Mar 01 00:00:00 EST 2017

Switching harmful visceral fat to beneficial energy combustion improves metabolic dysfunctions.

JCI insight

Yang X,Sui W,Zhang M,Dong M,Lim S,Seki T,Guo Z,Fischer C,Lu H,Zhang C,Yang J,Zhang M,Wang Y,Cao C,Gao Y,Zhao X,Sun M,Sun Y,Zhuang R,Samani NJ,Zhang Y,Cao Y

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Thu Feb 23 00:00:00 EST 2017

HMGA2 promotes vasculogenic mimicry and tumor aggressiveness by upregulating Twist1 in gastric carcinoma.

Scientific reports

Sun J,Sun B,Sun R,Zhu D,Zhao X,Zhang Y,Dong X,Che N,Li J,Liu F,Zhao N,Wang Y,Zhang D

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Mon May 22 00:00:00 EDT 2017

Conditional deletion of AP-2β in mouse cranial neural crest results in anterior segment dysgenesis and early-onset glaucoma.

Disease models & mechanisms

Martino VB,Sabljic T,Deschamps P,Green RM,Akula M,Peacock E,Ball A,Williams T,West-Mays JA

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry (Paraffin)

Mon Aug 01 00:00:00 EDT 2016

Co-option of pre-existing vascular beds in adipose tissue controls tumor growth rates and angiogenesis.

Oncotarget

Lim S,Hosaka K,Nakamura M,Cao Y

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunofluorescence

Tue Jun 21 00:00:00 EDT 2016

COX2 inhibition reduces aortic valve calcification in vivo.

Arteriosclerosis, thrombosis, and vascular biology

Wirrig EE,Gomez MV,Hinton RB,Yutzey KE

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunofluorescence

Wed Apr 01 00:00:00 EDT 2015

The myeloid-binding peptide adenoviral vector enables multi-organ vascular endothelial gene targeting.

Laboratory investigation; a journal of technical methods and pathology

Lu ZH,Kaliberov S,Zhang J,Muz B,Azab AK,Sohn RE,Kaliberova L,Du Y,Curiel DT,Arbeit JM

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunofluorescence

Fri Aug 01 00:00:00 EDT 2014

Transcriptional targeting of primary and metastatic tumor neovasculature by an adenoviral type 5 roundabout4 vector in mice.

PloS one

Lu ZH,Kaliberov S,Sohn RE,Kaliberova L,Curiel DT,Arbeit JM

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunofluorescence

Mon Mar 02 00:00:00 EST 2015

Loss of p53 attenuates the contribution of IL-6 deletion on suppressed tumor progression and extended survival in Kras-driven murine lung cancer.

PloS one

Tan X,Carretero J,Chen Z,Zhang J,Wang Y,Chen J,Li X,Ye H,Tang C,Cheng X,Hou N,Yang X,Wong KK

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunohistochemistry

Mon Jun 30 00:00:00 EDT 2014

Synergistic regulation of p53 by Mdm2 and Mdm4 is critical in cardiac endocardial cushion morphogenesis during heart development.

The Journal of pathology

Zhang Q,He X,Chen L,Zhang C,Gao X,Yang Z,Liu G

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunofluorescence

Thu Nov 01 00:00:00 EDT 2012

ETS-dependent regulation of a distal Gata4 cardiac enhancer.

Developmental biology

Schachterle W,Rojas A,Xu SM,Black BL

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunofluorescence

Sun Jan 15 00:00:00 EST 2012

PDK1 regulates vascular remodeling and promotes epithelial-mesenchymal transition in cardiac development.

Molecular and cellular biology

Feng Q,Di R,Tao F,Chang Z,Lu S,Fan W,Shan C,Li X,Yang Z

Published figure using Endomucin monoclonal antibody (Product # 50-5851-82) in Immunofluorescence

Thu Jul 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Endomucin; endomucin-1; Endomucin-1/2; MUC-14; mucin 14, endothelial; Mucin-14

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Gene Aliases: 0610012K22Rik; AI315669; Emcn; Muc14

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UniProt ID: (Mouse) Q9R0H2

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Entrez Gene ID: (Mouse) 59308

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Performance Guarantee

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Endomucin Monoclonal Antibody (eBioV.7C7 (V.7C7)), eFluor™ 660, eBioscience™

Endomucin Antibody (50-5851-82) in Flow

Product Details

50-5851-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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