Invitrogen

ERK1 Polyclonal Antibody

View all (57) ERK1 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-77818

Applications
Tested Dilution
Publications

Western Blot (WB)

1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:100
-

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

Assay-Dependent
-
Product Specifications

Species Reactivity

Bovine, Chicken, Fruit fly, Human, Mouse, Sheep, Rat, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 35 residue synthetic peptide, corresponding to rat Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735769

Product Specific Information

A 1:1,000 dilution of PA5-77818 was sufficient for detection of ERK1/2 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.|Detects approximately 44kda (ERK1) and approximately 42kDa (ERK2).

Target Information

MAPK3 (ERK1) is a serine/threonine kinase that is an important activator of p90, RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. ERK1 is widely expressed and involved in the regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. Functionally, ERK1 is involved with a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. ERK1 is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms of ERK1 have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ERK; ERK-1; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAP kinase2; MAPK 1; MAPK 3; MAPK2; Microtubule-associated protein 2 kinase; mitogen-activated 3; mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; MNK1; p44 MAP kinase; p44-ERK1; p44-MAPK; pp42/MAP kinase

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Gene Aliases: ERK-1; ERK1; ERT2; Esrk1; HS44KDAP; HUMKER1A; MAPK1; MAPK3; MNK1; Mtap2k; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3

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UniProt ID: (Human) P27361, (Rat) P21708, (Mouse) Q63844

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Entrez Gene ID: (Bovine) 531391, (Human) 5595, (Sheep) 101123008, (Rat) 50689, (Mouse) 26417

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Function(s)
phosphotyrosine binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding kinase activity phosphatase binding scaffold protein binding protein kinase activity nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade activation of MAPK activity MAPK import into nucleus positive regulation of protein phosphorylation transcription initiation from RNA polymerase I promoter protein complex assembly protein phosphorylation apoptotic process DNA damage induced protein phosphorylation cell cycle axon guidance fibroblast growth factor receptor signaling pathway response to toxic substance regulation of phosphatidylinositol 3-kinase signaling viral process phosphorylation peptidyl-serine phosphorylation sensory perception of pain arachidonic acid metabolic process platelet activation BMP signaling pathway thyroid gland development positive regulation of cyclase activity lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance via telomerase regulation of stress-activated MAPK cascade positive regulation of histone phosphorylation positive regulation of histone acetylation peptidyl-tyrosine autophosphorylation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis outer ear morphogenesis response to exogenous dsRNA positive regulation of translation positive regulation of transcription from RNA polymerase II promoter thymus development regulation of sequence-specific DNA binding transcription factor activity cartilage development regulation of cytoskeleton organization positive regulation of telomerase activity Bergmann glial cell differentiation face development JAK-STAT cascade involved in growth hormone signaling pathway lung morphogenesis trachea formation cardiac neural crest cell development involved in heart development ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade interleukin-1-mediated signaling pathway response to epidermal growth factor cellular response to mechanical stimulus caveolin-mediated endocytosis regulation of Golgi inheritance regulation of cellular response to heat positive regulation of telomere capping positive regulation of xenophagy regulation of early endosome to late endosome transport negative regulation of apolipoprotein binding transcription, DNA-templated cellular response to DNA damage stimulus organ morphogenesis response to lipopolysaccharide intracellular signal transduction positive regulation of transcription, DNA-templated signal transduction
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