Invitrogen

Dopamine Transporter Monoclonal Antibody (mAb16)

Dopamine Transporter Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Dopamine Transporter Antibody (MA5-24796) in IHC (P)

Immunohistochemical analysis of Dopamine Transporter in mouse brain. Samples were incubated in Dopamine Transporter monoclonal antibody (Product # MA5-24796) followed by DAB with hematoxylin counterstain.

Dopamine Transporter Antibody in Western Blot (WB)

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Product Details

MA5-24796

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

1:250

ELISA (ELISA)

Assay-Dependent

Immunoprecipitation (IP)

Assay-Dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

mAb16

Immunogen

Synthetic peptide corresponding to the N-terminus of rat DAT1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

TBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2717269

Target Information

The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Evaluation and Validation of Commercially Available Dopamine Transporter Antibodies.

eNeuro

Russo EE,Zovko LE,Nazari R,Steenland H,Ramsey AJ,Salahpour A

Published figure using Dopamine Transporter monoclonal antibody (Product # MA5-24796) in Western Blot

Mon May 01 00:00:00 EDT 2023

Recovery of dopaminergic system after cocaine exposure and impact of a long-acting cocaine hydrolase.

Addiction biology

Deng J,Zhang T,Zheng X,Shang L,Zhan CG,Zheng F

MA5-24796 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to demonstrate that a highly efficient Fc-fused cocaine hydrolase, CocH5-Fc(M6), effectively blocked cocaine-induced hyperactivity and DAT trafficking with repeated cocaine exposures by maintaining a plasma CocH5-Fc(M6) concentration ≥58.7 2.9 nM in rats.

Fri Jul 01 00:00:00 EDT 2022

Long-term RNAi knockdown of α-synuclein in the adult rat substantia nigra without neurodegeneration.

Neurobiology of disease

Zharikov A,Bai Q,De Miranda BR,Van Laar A,Greenamyre JT,Burton EA

MA5-24796 was used in Immunohistochemistry-immunofluorescence to investigate the impact of long-term α-synuclein knockdown in the adult rat central nervous system and its role in the pathogenesis of Parkinson''s disease.

Wed May 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: DA transporter; DAT; dopamine transporter 1; Sodium-dependent dopamine transporter; solute carrier family 6 (neurotransmitter transporter), member 3; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; Solute carrier family 6 member 3; solute carrier family 6, member 3

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Gene Aliases: DAT; DAT1; PKDYS; SLC6A3

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UniProt ID: (Human) Q01959, (Rat) P23977, (Mouse) Q61327

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Entrez Gene ID: (Human) 6531, (Rat) 24898, (Mouse) 13162

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Function(s)

protease binding receptor binding dopamine transmembrane transporter activity dopamine:sodium symporter activity protein binding drug binding monoamine transmembrane transporter activity protein complex binding dopamine binding metal ion binding protein N-terminus binding protein phosphatase 2A binding neurotransmitter:sodium symporter activity symporter activity primary active transporter

Process(es)

aging lactation sensory perception of smell locomotory behavior response to iron ion monoamine transport dopamine transport adenohypophysis development response to nicotine positive regulation of multicellular organism growth regulation of dopamine metabolic process neurotransmitter biosynthetic process response to cocaine dopamine biosynthetic process dopamine catabolic process response to drug response to ethanol dopamine uptake involved in synaptic transmission response to cAMP prepulse inhibition ammonium transmembrane transport dopamine uptake neurotransmitter transport response to organic cyclic compound cation transmembrane transport transport organic cation transport sodium ion transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Dopamine Transporter Monoclonal Antibody (mAb16)

Dopamine Transporter Antibody (MA5-24796) in IHC (P)

Product Details

MA5-24796

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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