Invitrogen

Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight™ 800

Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody in Western Blot (WB)

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Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (SA5-10044) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Caco-2 (Lane 1) and K-562 (Lane 2). The blots were probed with Anti-PRDX6 Recombinant Rabbit Monoclonal Antibody (Product # 702211, 2 µg/mL) and detected using Donkey Anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 800 (Product # SA5-10044) at dilutions 1:5,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. ... View More

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Product Details

SA5-10044

Applications

Tested Dilution

Western Blot (WB)

1:1,000-1:20,000

Immunohistochemistry (IHC)

1:50-1:2,000

Immunocytochemistry (ICC/IF)

1:50-1:2,000

Flow Cytometry (Flow)

1:50-1:200

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Rabbit IgG-heavy and light chain

Conjugate

DyLight™ 800 DyLight™ 800 DyLight™ 800

Excitation/Emission Max

789/794 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 6.8 to 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2556624

Target

IgG

Cross Adsorption

Against bovine, chicken, goat, human, mouse, pig and rat

Antibody Form

Whole Antibody

Product Specific Information

This antibody is cross-adsorbed and exhibits minimum reactivity to bovine, chicken, goat, human, mouse, pig and rat.

For Western blot applications, 5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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