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Invitrogen
Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (31458) in WB
Product Details
31458
Western Blot (WB)
Immunohistochemistry (IHC)
Immunocytochemistry (ICC/IF)
Species Reactivity
Host/Isotype
Class
Type
Conjugate
Form
Concentration
Purification
Storage buffer
Contains
Storage conditions
Shipping conditions
RRID
Target
Cross Adsorption
Antibody Form
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
This antibody has been successfully used in Western blot, and ICC applications.
Antibody Specificity: This antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Restoration and Storage: Store product at 2-8°C until opened. Restore with 0.5 mL distilled water (0.8 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at -70°C as an undiluted liquid. After dilution, do not use for more than one day.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Country of Origin: USA
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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