Invitrogen

Dengue Virus Type 2 NS3 Polyclonal Antibody

Dengue Virus Type 2 NS3 Antibody in Western Blot (WB)

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Dengue Virus Type 2 NS3 Antibody (PA5-32199) in WB

NS3 (Dengue virus) antibody detects NS3 (Dengue virus) protein by western blot analysis. A. 30 µg BHK-21 whole cell extract. B. 30 µg whole cell extract of Dengue virus type 1 infected BHK-21 cells. C. 30 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells. D. 30 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells. E. 30 µg whole cell extract of Dengue virus type 4 infected BHK... View More

Dengue Virus Type 2 NS3 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Dengue Virus Type 2 NS3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

PA5-32199

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:2,000

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 354 and 568 of Dengue virus NS3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2549672

Product Specific Information

Recommended positive controls: Dengue virus type 1 infected BHK-21, Dengue virus type 2 infected BHK-21, Dengue virus type 3 infected BHK-21, Dengue virus type 4 infected BHK-21.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Dengue fever and dengue hemorrhagic fever are acute febrile diseases, found in the tropics and Africa. Caused by one of four closely related virus serotypes of the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. Dengue is transmitted to humans by the Aedes aegypti (rarely Aedes albopictus) mosquito, which feeds during the day.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Zika virus alters centrosome organization to suppress the innate immune response.

EMBO reports

Kodani A,Knopp KA,Di Lullo E,Retallack H,Kriegstein AR,DeRisi JL,Reiter JF

PA5-32199 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to propose that ZIKV disrupts CEP63 function to increase centrosomal DTX4 localization and destabilization of TBK1, thereby tempering the innate immune response.

Mon Sep 05 00:00:00 EDT 2022

A slow, efficient and safe nanoplatform of tailored ZnS QD-mycophenolic acid conjugates for in vitro drug delivery against dengue virus 2 genome replication.

Nanoscale advances

Dungdung R,Bayal M,Valliyott L,Unniyampurath U,Nair SS,Pilankatta R

PA5-32199 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to indicate that the Selectivity Index 50 (SI50) of the ZnS QD-MPA conjugate was two orders higher than that of free MPA with lower cytotoxicity.

Tue Dec 15 00:00:00 EST 2020

A slow, efficient and safe nanoplatform of tailored ZnS QD-mycophenolic acid conjugates for in vitro drug delivery against dengue virus 2 genome replication.

Nanoscale advances

Dungdung R,Bayal M,Valliyott L,Unniyampurath U,Nair SS,Pilankatta R

Tue Dec 15 00:00:00 EST 2020

A slow, efficient and safe nanoplatform of tailored ZnS QD-mycophenolic acid conjugates for in vitro drug delivery against dengue virus 2 genome replication.

Nanoscale advances

Dungdung R,Bayal M,Valliyott L,Unniyampurath U,Nair SS,Pilankatta R

Tue Dec 15 00:00:00 EST 2020

Activity of vitamin D receptor agonists against dengue virus.

Scientific reports

Jaratsittisin J,Xu B,Sornjai W,Weng Z,Kuadkitkan A,Li F,Zhou GC,Smith DR

PA5-32199 was used in Western Blotting to study whether fused bicyclic derivatives of 1H-pyrrolo[1,2] imidazol-1-one with vitamin D receptor (VDR) agonist activity demonstrate anti-mosquito-transmitted dengue virus (DENV) activity when used as post-infection treatment.

Thu Jul 02 00:00:00 EDT 2020

Interferon-stimulated TRIM69 interrupts dengue virus replication by ubiquitinating viral nonstructural protein 3.

PLoS pathogens

Wang K,Zou C,Wang X,Huang C,Feng T,Pan W,Wu Q,Wang P,Dai J

Published figure using Dengue Virus Type 2 NS3 polyclonal antibody (Product # PA5-32199) in Western Blot

Wed Aug 01 00:00:00 EDT 2018

Interferon-stimulated TRIM69 interrupts dengue virus replication by ubiquitinating viral nonstructural protein 3.

PLoS pathogens

Wang K,Zou C,Wang X,Huang C,Feng T,Pan W,Wu Q,Wang P,Dai J

Published figure using Dengue Virus Type 2 NS3 polyclonal antibody (Product # PA5-32199) in Western Blot

Wed Aug 01 00:00:00 EDT 2018

Activity of andrographolide against dengue virus.

Antiviral research

Panraksa P,Ramphan S,Khongwichit S,Smith DR

PA532199 was used in immunocytochemistry and western blot to examine the antiviral activity of andrographolide against dengue virus serotype 2

Wed Mar 01 00:00:00 EST 2017

Involvement of voltage-dependent anion channel (VDAC) in dengue infection.

Scientific reports

Jitobaom K,Tongluan N,Smith DR

PA532199 was used in western blot to study the role of the voltage-dependent anion channel during dengue virus infection

Tue Oct 25 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: Dengue NS3; Dengue NS3 protein

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Dengue Virus Type 2 NS3 Polyclonal Antibody

Dengue Virus Type 2 NS3 Antibody (PA5-32199) in WB

Product Details

PA5-32199

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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