Invitrogen

DYKDDDDK Tag Polyclonal Antibody, HRP

DYKDDDDK Tag Antibody in Western Blot (WB)

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DYKDDDDK Tag Antibody (PA1-984B-HRP) in WB

Western blot analysis of FLAG® Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were probed with FLAG-tag polyclonal antibody (Product # PA1-984B-HRP) at a dilution of 1:500 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using Super Signal West ... View More

DYKDDDDK Tag Antibody in Western Blot, ChIP Assay (WB, ChIP)

DYKDDDDK Tag Antibody in Immunocytochemistry (ICC/IF)

DYKDDDDK Tag Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

DYKDDDDK Tag Antibody in Immunohistochemistry (IHC)

DYKDDDDK Tag Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

PA1-984B-HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues DYKDDDDK

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with proprietary stabilizer

Contains

0.07% Kathon

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610669

Product Specific Information

PA1-983B-HRP detects Flag-tagged proteins in Western Blot applications.

Similar epitope as Flag tag from Sigma.

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Proteomic Identification of Coxiella burnetii Effector Proteins Targeted to the Host Cell Mitochondria During Infection.

Molecular & cellular proteomics : MCP

Fielden LF,Scott NE,Palmer CS,Khoo CA,Newton HJ,Stojanovski D

Published figure using DYKDDDDK Tag polyclonal antibody (Product # PA1-984B-HRP) in Western Blot

Mon Mar 14 00:00:00 EDT 2022

Proteomic Identification of Coxiella burnetii Effector Proteins Targeted to the Host Cell Mitochondria During Infection.

Molecular & cellular proteomics : MCP

Fielden LF,Scott NE,Palmer CS,Khoo CA,Newton HJ,Stojanovski D

Published figure using DYKDDDDK Tag polyclonal antibody (Product # PA1-984B-HRP) in Western Blot

Mon Mar 14 00:00:00 EDT 2022

Phosphorylation of Connexin36 near the C-terminus switches binding affinities for PDZ-domain and 14-3-3 proteins in vitro.

Scientific reports

Tetenborg S,Wang HY,Nemitz L,Depping A,Espejo AB,Aseervatham J,Bedford MT,Janssen-Bienhold U,O'Brien J,Dedek K

Published figure using DYKDDDDK Tag polyclonal antibody (Product # PA1-984B-HRP) in Immunocytochemistry

Tue Oct 27 00:00:00 EDT 2020

An efficient and quantitative assay for epitope-tagged therapeutic protein development with a capillary western system.

Bioanalysis

Li Y,Duah E,Long N,Persaud A,VanGundy Z,Magliery T,Poi MJ

PA1-984B-HRP was used in Simple Western - Size to develop and validate a reliable, robust and efficient assay to detect and quantify biologic compounds in vitro and in vivo during early stage of a biotherapeutic agent discovery.

Fri Mar 01 00:00:00 EST 2019

Identification and Characterization of USP7 Targets in Cancer Cells.

Scientific reports

Georges A,Marcon E,Greenblatt J,Frappier L

Published figure using DYKDDDDK Tag polyclonal antibody (Product # PA1-984B-HRP) in Western Blot

Fri Oct 26 00:00:00 EDT 2018

Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.

Acta neuropathologica communications

Frick P,Sellier C,Mackenzie IRA,Cheng CY,Tahraoui-Bories J,Martinat C,Pasterkamp RJ,Prudlo J,Edbauer D,Oulad-Abdelghani M,Feederle R,Charlet-Berguerand N,Neumann M

Published figure using DYKDDDDK Tag polyclonal antibody (Product # PA1-984B-HRP) in Western Blot

Fri Aug 03 00:00:00 EDT 2018

Methylation of the phosphatase-transcription activator EYA1 by protein arginine methyltransferase 1: mechanistic, functional, and structural studies.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li X,Eberhardt A,Hansen JN,Bohmann D,Li H,Schor NF

Published figure using DYKDDDDK Tag polyclonal antibody (Product # PA1-984B-HRP) in Immunoprecipitation

Thu Jun 01 00:00:00 EDT 2017

Selective regulation of Mmp13 by 1,25(OH)₂D₃, PTH, and Osterix through distal enhancers.

The Journal of steroid biochemistry and molecular biology

Meyer MB,Benkusky NA,Onal M,Pike JW

Published figure using DYKDDDDK Tag polyclonal antibody (Product # PA1-984B-HRP) in ChIP assay

Tue Nov 01 00:00:00 EDT 2016

Selective regulation of Mmp13 by 1,25(OH)₂D₃, PTH, and Osterix through distal enhancers.

The Journal of steroid biochemistry and molecular biology

Meyer MB,Benkusky NA,Onal M,Pike JW

Published figure using DYKDDDDK Tag polyclonal antibody (Product # PA1-984B-HRP) in ChIP assay

Tue Nov 01 00:00:00 EDT 2016

The production of viral vectors designed to express large and difficult to express transgenes within neurons.

Molecular brain

Holehonnur R,Lella SK,Ho A,Luong JA,Ploski JE

Published figure using DYKDDDDK Tag polyclonal antibody (Product # PA1-984B-HRP) in Immunofluorescence

Tue Feb 24 00:00:00 EST 2015

The production of viral vectors designed to express large and difficult to express transgenes within neurons.

Molecular brain

Holehonnur R,Lella SK,Ho A,Luong JA,Ploski JE

Published figure using DYKDDDDK Tag polyclonal antibody (Product # PA1-984B-HRP) in Immunofluorescence

Tue Feb 24 00:00:00 EST 2015

Distinct roles of neuroligin-1 and SynCAM1 in synapse formation and function in primary hippocampal neuronal cultures.

Neuroscience

Burton SD,Johnson JW,Zeringue HC,Meriney SD

Published figure using DYKDDDDK Tag polyclonal antibody (Product # PA1-984B-HRP) in Western Blot

Thu Jul 26 00:00:00 EDT 2012

Virus-infection or 5'ppp-RNA activates antiviral signal through redistribution of IPS-1 mediated by MFN1.

PLoS pathogens

Onoguchi K,Onomoto K,Takamatsu S,Jogi M,Takemura A,Morimoto S,Julkunen I,Namiki H,Yoneyama M,Fujita T

Published figure using DYKDDDDK Tag polyclonal antibody (Product # PA1-984B-HRP) in Western Blot

Thu Jul 22 00:00:00 EDT 2010

Virus-infection or 5'ppp-RNA activates antiviral signal through redistribution of IPS-1 mediated by MFN1.

PLoS pathogens

Onoguchi K,Onomoto K,Takamatsu S,Jogi M,Takemura A,Morimoto S,Julkunen I,Namiki H,Yoneyama M,Fujita T

Published figure using DYKDDDDK Tag polyclonal antibody (Product # PA1-984B-HRP) in Western Blot

Thu Jul 22 00:00:00 EDT 2010

Virus-infection or 5'ppp-RNA activates antiviral signal through redistribution of IPS-1 mediated by MFN1.

PLoS pathogens

Onoguchi K,Onomoto K,Takamatsu S,Jogi M,Takemura A,Morimoto S,Julkunen I,Namiki H,Yoneyama M,Fujita T

Published figure using DYKDDDDK Tag polyclonal antibody (Product # PA1-984B-HRP) in Western Blot

Thu Jul 22 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DYKDDDDK Tag Polyclonal Antibody, HRP

DYKDDDDK Tag Antibody (PA1-984B-HRP) in WB

Product Details

PA1-984B-HRP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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