Invitrogen

DR4 Monoclonal Antibody (32A242)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Datasheet

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Datasheet

Protocols

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FIGURE: 1 / 4

DR4 Antibody (MA1-41049) in WB

Western Blot was performed using Anti-DR4 Monoclonal Antibody (32A242) (Product # MA1-41049) and a 54 kDa band corresponding to Tumor necrosis factor receptor superfamily member 10A was observed across tested samples. Membrane enriched extracts (40 µg lysate) of MDA-MB-231 (Lane 1), T-47D (Lane 2), MCF7 (Lane 3), A549 (Lane 4), HeLa (Lane 5), Caco-2 (Lane 6), IMR-32 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # ... View More

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DR4 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of Tumor necrosis factor receptor superfamily member 10A was observed in MDA-MB-231, A549 compared to MCF7 and IMR-32 using Anti-DR4 Monoclonal Antibody (32A242) (Product # MA1-41049) in Western Blot (DOI: 10.1158/1541-7786.MCR-08-0313). {RE}

Product Details

MA1-41049

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

Immunocytochemistry (ICC/IF)

Assay-Dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

32A242

Immunogen

Synthetic peptide corresponding to residues 2-21 of mature human DR4.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1088005

Product Specific Information

Suggested positive control: Hela whole cell extract, HeLa cell lysate.

Target Information

TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues. Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD261; cytotoxic TRAIL receptor; Death receptor 4; MGC9365; sCD261; soluble CD261; soluble TRAIL; sTRAIL; TNF-related apoptosis-inducing ligand receptor 1; TRAIL R1; TRAIL receptor 1; TRAIL-R1; Tumor necrosis factor receptor superfamily member 10A; tumor necrosis factor receptor superfamily member 10a variant 2; tumor necrosis factor receptor superfamily, member 10a

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Gene Aliases: APO2; CD261; DR4; TNFRSF10A; TRAILR-1; TRAILR1

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UniProt ID: (Human) O00220

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Entrez Gene ID: (Human) 8797

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Function(s)

protease binding receptor activity tumor necrosis factor-activated receptor activity death receptor activity protein binding transcription factor binding TRAIL binding transmembrane signal receptor

Process(es)

apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response immune response signal transduction cell surface receptor signaling pathway activation of NF-kappaB-inducing kinase activity multicellular organism development extrinsic apoptotic signaling pathway via death domain receptors response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway TRAIL-activated apoptotic signaling pathway regulation of cell proliferation regulation of apoptotic process cellular response to mechanical stimulus extrinsic apoptotic signaling pathway activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of extrinsic apoptotic signaling pathway via death domain receptors

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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DR4 Monoclonal Antibody (32A242)

DR4 Antibody (MA1-41049) in WB

Product Details

MA1-41049

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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