Invitrogen

CXCR4 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

2 References

View all (76) CXCR4 (CD184) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 4

CXCR4 Antibody (PA5-19857) in WB

Antioxidants (Basel, Switzerland) 2022 - The CXCR4 expression of oxaliplatin-resistant CRC HCT-116/R cells and the parental cell line HCT-116. ( A ) Real-time polymerase chain reaction analysis of CXCR4 mRNA expression in HCT-116 and HCT-116/R cells. ( B ) Western blot analysis for the expression of CXCR4 in HCT-116 and HCT-116/R cells. * p < 0.01 vs. HCT-116/R cells.

Product Image Gallery

CXCR4 Antibody — Figure 2 CS increases expression of HIV receptors CD4 and CCR5 in NHBE ALI cultures. NHBE ALI cultures were exposed to CS and total protein was extracted. Cells were lysed with RIPA buffer containing protease inhibitor cocktail and protein expression was quantified by Western blot analysis and normalized using beta-actin. CS significantly enhances expression of CD4 (panel a) and CCR5 protein expression (panel b) when compared to air-exposed controls. CS does not increase CXCR4 protein expression (panel c). Western blot images ( a - c ) are representative of NHBE ALI cultures from three independent lungs. Relative density of the detected protein band was measured by using the ImageJ software and the values obtained were averaged. n = 3 lungs (unless stated otherwise). *Significant (p < 0.05). {TM}

Product Details

PA5-19857

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 15 amino acid peptide near the center of human CXCR4 isoform b

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153282

Product Specific Information

A suggested positive control is Hela cell lysate.

PA5-19857 can be used with blocking peptide PEP-0002.

The PA5-19857 immunogen is located within amino acids 170-220 of CXCR4.

Predicted molecular ~ 40kD. In Western blot applications, this antibody has been observed to detect a band at: 44kD (Post-modification: 2 N-linked glycosylation)

Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (86%), Sheep: (86%), Mouse: (73%), Rat: (67%)

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fermented Ginger Extract in Natural Deep Eutectic Solvent Enhances Cytotoxicity by Inhibiting NF-κB Mediated CXC Chemokine Receptor 4 Expression in Oxaliplatin-Resistant Human Colorectal Cancer Cells.

Antioxidants (Basel, Switzerland)

Lee KC,Wu KL,Chang SF,Chang HI,Chen CN,Chen YY

Published figure using CXCR4 polyclonal antibody (Product # PA5-19857) in Western Blot

Wed Oct 19 00:00:00 EDT 2022

Cigarette smoke promotes HIV infection of primary bronchial epithelium and additively suppresses CFTR function.

Scientific reports

Chinnapaiyan S,Dutta R,Bala J,Parira T,Agudelo M,Nair M,Unwalla HJ

Published figure using CXCR4 polyclonal antibody (Product # PA5-19857) in Western Blot

Tue May 22 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C) receptor 4; chemokine receptor; chemokine receptor (LCR1); chemokine receptor 4; CXC; CXC chemokine receptor; CXC motif chemokine; CXC-R4; CXCR-4; FB22; Fusin; HM89; HUMSTR; LAP-3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; leukocyte-expressed seven-transmembrane-domain; Lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPYRL; PB-CKR; Pre-B-cell-derived chemokine receptor; SDF-1 receptor; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor

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Gene Aliases: b2b220Clo; CD184; Cmkar4; CXCR4; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; PB-CKR; PBSF/SDF-1; Sdf1r; WHIM; WHIMS

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UniProt ID: (Human) P61073, (Mouse) P70658, (Rat) O08565

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Entrez Gene ID: (Human) 7852, (Mouse) 12767, (Rat) 60628

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Function(s)

virus receptor activity actin binding G-protein coupled receptor activity protein binding drug binding coreceptor activity C-X-C chemokine receptor activity cytokine binding ubiquitin protein ligase binding myosin light chain binding ubiquitin binding signal transducer activity chemokine receptor activity C-C chemokine receptor activity

Process(es)

patterning of blood vessels ameboidal-type cell migration neuron migration epithelial cell development chemotaxis signal transduction G-protein coupled receptor signaling pathway germ cell development nervous system development brain development motor neuron axon guidance germ cell migration organ morphogenesis cell migration calcium-mediated signaling telencephalon cell migration regulation of cell migration T cell proliferation neutrophil activation regulation of programmed cell death myelin maintenance positive regulation of oligodendrocyte differentiation regulation of chemotaxis regulation of calcium ion transport cardiac muscle contraction chemokine-mediated signaling pathway cellular response to cytokine stimulus response to hypoxia neuron recognition neural precursor cell proliferation activation of MAPK activity dendritic cell chemotaxis apoptotic process inflammatory response positive regulation of cytosolic calcium ion concentration response to virus fusion of virus membrane with host plasma membrane entry into host cell

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CXCR4 Polyclonal Antibody

CXCR4 Antibody (PA5-19857) in WB

Product Details

PA5-19857

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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