Invitrogen

CDK2 Recombinant Rabbit Monoclonal Antibody (ARC0222)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

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FIGURE: 1 / 6

CDK2 Antibody (MA5-35045) in WB

Western blot analysis of CDK2 in extracts of various cell lines. Samples were incubated with CDK2 Monoclonal antibody (Product # MA5-35045) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

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CDK2 Antibody — Antibody specificity was demonstrated by knockout of the target protein. Loss of signal was observed for target protein in CDK2 knockout (KO) 293T cell line using Anti-CDK2 Recombinant Rabbit Monoclonal Antibody (ARC0222) (Product # MA5-35045). {KO}

Product Details

MA5-35045

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC0222

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 199-298 of human CDK2 (P24941)

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2848950

Product Specific Information

Immunogen sequence: RRALFPGDSE IDQLFRIFRT LGTPDEVVWP GVTSMPDYKP SFPKWARQDF SKVVPPLDED GRSLLSQMLH YDPNKRISAK AALAHPFFQD VTKPVPHLRL

Target Information

CDK2/cyclinO is a member of the cyclin dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene which is localized exclusively in the nucleus. In addition, CyclinO was reported to shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and the protein is turned over during the course of cell cycle. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cdc2-related protein kinase; CDC28; CDC2A; Cell division protein kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; MPF; p33 protein kinase; p33CDK2

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Gene Aliases: A630093N05Rik; CDK2; CDKN2; p33(CDK2)

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UniProt ID: (Human) P24941, (Mouse) P97377

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Entrez Gene ID: (Human) 1017, (Rat) 362817, (Mouse) 12566

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Function(s)

protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding kinase activity cyclin binding protein complex binding histone kinase activity metal ion binding protein kinase activity nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase

Process(es)

G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle lens development in camera-type eye DNA replication DNA repair potassium ion transport DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic nuclear division centriole replication meiotic nuclear division Ras protein signal transduction positive regulation of cell proliferation response to toxic substance histone phosphorylation peptidyl-serine phosphorylation organ regeneration mitotic G1 DNA damage checkpoint positive regulation of DNA-dependent DNA replication initiation response to estradiol cellular response to insulin stimulus response to drug response to ethanol positive regulation of transcription, DNA-templated response to cadmium ion centrosome duplication cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle response to cAMP response to electrical stimulus regulation of gene silencing cellular response to nitric oxide regulation of signal transduction by p53 class mediator protein phosphorylation meiotic cell cycle regulation of cell cycle cellular response to DNA damage stimulus cell cycle phosphorylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CDK2 Recombinant Rabbit Monoclonal Antibody (ARC0222)

CDK2 Antibody (MA5-35045) in WB

Product Details

MA5-35045

Western Blot (WB)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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