Proteintech

CDK2 Monoclonal Antibody (4E2E10)

CDK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CDK2 Antibody (60312-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using 60312-1-IG ( CDK2 Antibody) at dilution of 1:50 (under 40x lens).

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Product Details

60312-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

4E2E10

Immunogen

CDK2 Fusion Protein Ag21310 (8-210 aa encoded by BC003065)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Caprylic acid precipitation

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: EKIGEGTYG VVYKARNKLT GEVVALKKIR LDTETEGVPS TAIREISLLK ELNHPNIVKL LDVIHTENKL YLVFEFLHQD LKKFMDASAL TGIPLPLIKS YLFQLLQGLA FCHSHRVLHR DLKPQNLLIN TEGAIKLADF GLARAFGVPV RTYTHEVVTL WYRAPEILLG CKYYSTAVDI WSLGCIFAEM VTRRALFPGD SEID (8-210 aa encoded by BC003065)

Target Information

CDK2/cyclinO is a member of the cyclin dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene which is localized exclusively in the nucleus. In addition, CyclinO was reported to shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and the protein is turned over during the course of cell cycle. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Ebastine exerts antitumor activity and induces autophagy by activating AMPK/ULK1 signaling in an IPMK-dependent manner in osteosarcoma.

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The Journal of pharmacy and pharmacology

Yin W,Fu X,Chang W,Han L,Meng J,Cao A,Ren X,Fan Z,Zhou S

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The role of ER stress and ATP/AMPK in oxidative stress meditated hepatotoxicity induced by citrinin.

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Wu J,Yang C,Yang M,Liang Z,Wu Y,Kong X,Fan H,Wang S,Ning C,Xiao W,Jin Y,Yi J,Yuan Z

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Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening.

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Wang L,Li C,Chen P,Liu C,Cui Z,Deng S,Yang H

Fri Jul 08 00:00:00 EDT 2022

Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65.

Chemico-biological interactions

Tan J,Sun M,Yin J,Zhou Q,Zhao R,Chen Q,Sun H,Jiang C,Li S,He Y

Thu Dec 01 00:00:00 EST 2022

MICAL2 Contributes to Gastric Cancer Cell Proliferation by Promoting YAP Dephosphorylation and Nuclear Translocation.

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Qi C,Min P,Wang Q,Wang Y,Song Y,Zhang Y,Bibi M,Du J

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CYP2C8 Suppress Proliferation, Migration, Invasion and Sorafenib Resistance of Hepatocellular Carcinoma via PI3K/Akt/p27^kip1 Axis.

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Thu Apr 28 00:00:00 EDT 2022

Discovery and Verification of Key Liver Cancer Genes and Alternative Splicing Events Based on Second-Generation Sequencing Data Analysis.

Biological & pharmaceutical bulletin

Cui M,Bai M,Zheng L,Bao Y,Sun L,Yu C,Sun Y,Song Z,Wang G,Yu Z,Li Y,Huang Y

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Bovine Milk Exosomes Affect Proliferation and Protect Macrophages against Cisplatin-Induced Cytotoxicity.

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Matic S,D'Souza DH,Wu T,Pangloli P,Dia VP

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LncRNA FEZF1-AS1 Promotes TGF-β2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04.

Journal of ophthalmology

Wang Y,Chen L,Gu Y,Wang Y,Yuan Y,Zhu Q,Bi M,Gu S

Wed Sep 30 00:00:00 EDT 2020

Poly(rC) binding protein 2 (PCBP2) promotes the viability of human gastric cancer cells by regulating CDK2.

FEBS open bio

Chen C,Lei J,Zheng Q,Tan S,Ding K,Yu C

Tue May 01 00:00:00 EDT 2018

Poly(rC) binding protein 2 (PCBP2) promotes the viability of human gastric cancer cells by regulating CDK2.

FEBS open bio

Chen C,Lei J,Zheng Q,Tan S,Ding K,Yu C

Tue May 01 00:00:00 EDT 2018

Poly(rC) binding protein 2 (PCBP2) promotes the viability of human gastric cancer cells by regulating CDK2.

FEBS open bio

Chen C,Lei J,Zheng Q,Tan S,Ding K,Yu C

Tue May 01 00:00:00 EDT 2018

Poly(rC) binding protein 2 (PCBP2) promotes the viability of human gastric cancer cells by regulating CDK2.

FEBS open bio

Chen C,Lei J,Zheng Q,Tan S,Ding K,Yu C

Tue May 01 00:00:00 EDT 2018

The role of the ATM/Chk/P53 pathway in mediating DNA damage in hand-foot syndrome induced by PLD.

Toxicology letters

Yang J,Qiao L,Zeng Z,Wang J,Zhu T,Wei J,Wu M,Ye S,Huang X,Ma D,Liu R,Gao Q

Wed Jan 04 00:00:00 EST 2017

The role of the ATM/Chk/P53 pathway in mediating DNA damage in hand-foot syndrome induced by PLD.

Toxicology letters

Yang J,Qiao L,Zeng Z,Wang J,Zhu T,Wei J,Wu M,Ye S,Huang X,Ma D,Liu R,Gao Q

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High expression of N-myc (and STAT) interactor predicts poor prognosis and promotes tumor growth in human glioblastoma.

Oncotarget

Meng D,Chen Y,Yun D,Zhao Y,Wang J,Xu T,Li X,Wang Y,Yuan L,Sun R,Song X,Huai C,Hu L,Yang S,Min T,Chen J,Chen H,Lu D

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High expression of N-myc (and STAT) interactor predicts poor prognosis and promotes tumor growth in human glioblastoma.

Oncotarget

Meng D,Chen Y,Yun D,Zhao Y,Wang J,Xu T,Li X,Wang Y,Yuan L,Sun R,Song X,Huai C,Hu L,Yang S,Min T,Chen J,Chen H,Lu D

Tue Mar 10 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: cdc2-related protein kinase; CDC28; CDC2A; Cell division protein kinase 2; Cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; MPF; p33 protein kinase; p33CDK2

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Gene Aliases: CDK2; CDKN2; p33(CDK2)

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UniProt ID: (Human) P24941

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Entrez Gene ID: (Human) 1017

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Function(s)

protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding kinase activity cyclin binding protein complex binding histone kinase activity metal ion binding non-receptor serine/threonine protein kinase

Process(es)

G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle lens development in camera-type eye DNA replication DNA repair potassium ion transport DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic nuclear division centriole replication meiotic nuclear division Ras protein signal transduction positive regulation of cell proliferation response to toxic substance histone phosphorylation peptidyl-serine phosphorylation organ regeneration mitotic G1 DNA damage checkpoint positive regulation of DNA-dependent DNA replication initiation response to estradiol cellular response to insulin stimulus response to drug response to ethanol positive regulation of transcription, DNA-templated response to cadmium ion centrosome duplication cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle response to cAMP response to electrical stimulus regulation of gene silencing cellular response to nitric oxide regulation of signal transduction by p53 class mediator

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CDK2 Monoclonal Antibody (4E2E10)

CDK2 Antibody (60312-1-IG) in IHC (P)

Product Details

60312-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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