Invitrogen

CDH17 Monoclonal Antibody (05)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CDH17 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

CDH17 Antibody (MA5-29135) in ICC/IF

Immunofluorescence analysis of Cadherin-17 was performed using 70% confluent log phase Caco-2 and A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CDH17 Monoclonal Antibody (Product # MA5-29135) at 1:100 in 0.1% BSA, incubated at 4-degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Produc... View More

CDH17 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CDH17 Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-CDH17 Monoclonal Antibody (5) (Product # MA5-29135), shows in Plasma membrane localization in Caco-2 cells as compared to A-431 cells which is reported to be low to negative for CDH17 expression. {RE}

Product Details

MA5-29135

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

Immunogen

Recombinant Human Cadherin-17/LI-cadherin/CDH17 protein (Met1-Met787)

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2785058

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human Cadherin-17/LI-cadherin/CDH17.

Target Information

CDH17 gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cadherin; cadherin 17, LI cadherin (liver-intestine); cadherin-16; Cadherin-17; FLJ26931; HPT-1 cadherin; human intestinal peptide-associated transporter HPT-1; human peptide transporter 1; Intestinal peptide-associated transporter HPT-1; LI cadherin; LI-cadherin; Liver-intestine cadherin; MGC138218; MGC142024

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Gene Aliases: CDH16; CDH17; HPT-1; HPT1

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UniProt ID: (Human) Q12864

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Entrez Gene ID: (Human) 1015

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Function(s)

integrin binding transporter activity proton-dependent oligopeptide secondary active transmembrane transporter activity calcium ion binding cadherin

Process(es)

germinal center B cell differentiation marginal zone B cell differentiation transport oligopeptide transport cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules integrin-mediated signaling pathway calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules positive regulation of integrin activation by cell surface receptor linked signal transduction adherens junction organization oligopeptide transmembrane transport spleen development

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CDH17 Monoclonal Antibody (05)

CDH17 Antibody (MA5-29135) in ICC/IF

Product Details

MA5-29135

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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