Invitrogen

CD8 Monoclonal Antibody (3B5), Pacific Blue™

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CD8 Antibody (MHCD0828TR) in Flow

Figure 1. Multicolor analysis of CD4-positive and CD8-positive populations using Qdot® 605 streptavidin and a Pacific Blue™ dye-labeled conjugate. Human mononuclear cells were blocked with nonspecific goat IgG and then stained with a biotinylated mouse anti-human CD4 antibody. Cells were incubated with Qdot® 605 streptavidin, then with mouse anti-human CD8 antibody conjugated to Pacific Blue™ dye. Cells were analyzed on a flow cytometer equipped with a 405 nm violet diode laser and 450/50 and 605/20 nm bandpass filters. Compensation was pe... View More

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Product Details

MHCD0828TR

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 13 publications 13 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3B5

Immunogen

Recognizes the CD8 antigen

Conjugate

Pacific Blue™ Pacific Blue™ Pacific Blue™

Excitation/Emission Max

404/455 nm View spectra

Form

Liquid

Concentration

0.25 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2539727

Product Specific Information

For fluorochrome conjugated antibodies only, light exposure should be avoided. Use dim light during handling, incubation with cells, and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.

Each lot is tested by flow cytometry using human peripheral blood leukocytes (PBL). Based on this testing it is recommended that 5 µL of antibody be used per 1 x 10^6 cells in a 100 µL staining volume.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Droplet-microfluidics-assisted sequencing of HIV proviruses and their integration sites in cells from people on antiretroviral therapy.

Nature biomedical engineering

Sun C,Liu L,Pérez L,Li X,Liu Y,Xu P,Boritz EA,Mullins JI,Abate AR

MHCD0828 was used in Flow cytometry/Cell sorting to show that full-length proviruses connected to their contiguous HIV-host DNA junctions can be assembled via a high-throughput microfluidic assay where droplet-based whole-genome amplification of HIV DNA in its native context is followed by a polymerase chain reaction (PCR) to tag droplets containing proviruses for sequencing.

Mon Aug 01 00:00:00 EDT 2022

Pilot trial of topical MTS‑01 application to reduce dermatitis in patients receiving chemoradiotherapy for stage I‑III carcinoma of the anal canal.

International journal of oncology

Citrin D,Valle L,Camphausen K,Cooley-Zgela T,Smart D,Yao M,Mitchell JB,Thompson W,Sereti I,Uldrick T

MHCD0828 was used in flow cytometry experiments to demonstrate that the MTS-01 topical application was tolerable with minimal toxicity.

Wed Jun 01 00:00:00 EDT 2022

A Framework to Identify Antigen-Expanded T Cell Receptor Clusters Within Complex Repertoires.

Frontiers in immunology

Ceglia V,Kelley EJ,Boyle AS,Zurawski S,Mead HL,Harms CE,Blanck JP,Flamar AL,Kirschman JH,Ogongo P,Ernst JD,Levy Y,Zurawski G,Altin JA

MHCD0828 was used in flow cytometry to show that clonotypes within each CET respond to the same epitope, and that protein antigens stimulate multiple CETs reactive to constituent peptides.

Fri Feb 04 00:00:00 EST 2022

Anti-CD40 Antibody Fused to CD40 Ligand Is a Superagonist Platform for Adjuvant Intrinsic DC-Targeting Vaccines.

Frontiers in immunology

Ceglia V,Zurawski S,Montes M,Kroll M,Bouteau A,Wang Z,Ellis J,Igyártó BZ,Lévy Y,Zurawski G

MHCD0828 was used in Flow Cytometry to demonstrate that human CD40-CD40L antibody fused to antigens maintains highly agonistic activity and generates immune responses distinct from existing low agonist anti-CD40 targeting formats.

Thu Feb 24 00:00:00 EST 2022

Anti-CD40 Antibody Fused to CD40 Ligand Is a Superagonist Platform for Adjuvant Intrinsic DC-Targeting Vaccines.

Frontiers in immunology

Ceglia V,Zurawski S,Montes M,Kroll M,Bouteau A,Wang Z,Ellis J,Igyártó BZ,Lévy Y,Zurawski G

Thu Feb 24 00:00:00 EST 2022

Clonal replacement of tumor-specific T cells following PD-1 blockade.

Nature medicine

Yost KE,Satpathy AT,Wells DK,Qi Y,Wang C,Kageyama R,McNamara KL,Granja JM,Sarin KY,Brown RA,Gupta RK,Curtis C,Bucktrout SL,Davis MM,Chang ALS,Chang HY

MHCD0828 was used in Flow cytometry/Cell sorting to demonstrate that pre-existing tumor-specific T cells may have limited reinvigoration capacity, and that the T cell response to checkpoint blockade derives from a distinct repertoire of T cell clones that may have just recently entered the tumor.

Thu Aug 01 00:00:00 EDT 2019

Poly(I:C) primes primary human glioblastoma cells for an immune response invigorated by PD-L1 blockade.

Oncoimmunology

De Waele J,Marcq E,Van Audenaerde JR,Van Loenhout J,Deben C,Zwaenepoel K,Van de Kelft E,Van der Planken D,Menovsky T,Van den Bergh JM,Willemen Y,Pauwels P,Berneman ZN,Lardon F,Peeters M,Wouters A,Smits EL

MHCD0828 was used in Flow cytometry/Cell sorting to study the combination of poly(I:C) and PD-L1 blockade to invigorate the immune checkpoint inhibition response in glioblastoma.

Tue Jan 12 00:00:00 EST 2021

The tumor-associated antigen RHAMM (HMMR/CD168) is expressed by monocyte-derived dendritic cells and presented to T cells.

Oncotarget

Willemen Y,Van den Bergh JM,Bonte SM,Anguille S,Heirman C,Stein BM,Goossens H,Kerre T,Thielemans K,Peeters M,Van Tendeloo VF,Smits EL,Berneman ZN

MHCD0828 was used in flow cytometry to assess receptor for hyaluronic acid-mediated motility specific dendritic cell responses

Tue Nov 08 00:00:00 EST 2016

Concomitant evaluation of PMA+ionomycin-induced kinase phosphorylation and cytokine production in T cell subsets by flow cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Crawford TQ,Jalbert E,Ndhlovu LC,Barbour JD

Published figure using CD8 monoclonal antibody (Product # MHCD0828) in Flow Cytometry

Sat Mar 01 00:00:00 EST 2014

Interleukin-7 facilitates HIV-1 transmission to cervico-vaginal tissue ex vivo.

PLoS pathogens

Introini A,Vanpouille C,Lisco A,Grivel JC,Margolis L

MHCD0828TR was used in flow cytometry to study facilitation of HIV-1 transmission to cervico-vaginal tissue ex vivo by interleukin-7

Fri Feb 01 00:00:00 EST 2013

Establishment of a neonatal rhesus macaque model to study Mycobacterium tuberculosis infection.

Tuberculosis (Edinburgh, Scotland)

Cepeda M,Salas M,Folwarczny J,Leandro AC,Hodara VL,de la Garza MA,Dick EJ,Owston M,Armitige LY,Gauduin MC

MHCD0828 was used in flow cytometry to develop a newborn/infant model to study infection with Mycobacterium tuberculosis

Sun Dec 01 00:00:00 EST 2013

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

Published figure using CD8 monoclonal antibody (Product # MHCD0828) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

A CD8α(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities.

Immunology

Vargas-Inchaustegui DA,Demberg T,Robert-Guroff M

MHCD0828 was used in flow cytometry to characterize different macaque NK cell subpopulations.

Tue Nov 01 00:00:00 EDT 2011

HIV-1 infection abrogates CD8+ T cell mitogen-activated protein kinase signaling responses.

Journal of virology

Crawford TQ,Ndhlovu LC,Tan A,Carvidi A,Hecht FM,Sinclair E,Barbour JD

MHCD0828 was used in flow cytometry to elucidate relationships among MAPK pathway function, HIV-1-induced activation, and exhaustion markers in circulating CD8+ T cells

Thu Dec 01 00:00:00 EST 2011

Modulation of cell signaling networks after CTLA4 blockade in patients with metastatic melanoma.

PloS one

Comin-Anduix B,Sazegar H,Chodon T,Matsunaga D,Jalil J,von Euw E,Escuin-Ordinas H,Balderas R,Chmielowski B,Gomez-Navarro J,Koya RC,Ribas A

MHCD0828 was used in flow cytometry to assess the effects on cell signaling networks upon blockade of cytotoxic T lymphocyte-associated antigen-4 in patients with metastatic melanoma

Wed Sep 15 00:00:00 EDT 2010

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CD8 Monoclonal Antibody (3B5), Pacific Blue™

CD8 Antibody (MHCD0828TR) in Flow

Product Details

MHCD0828TR

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Performance Guarantee

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