Invitrogen

CD8 Monoclonal Antibody (3B5), PE

FIGURE: 1 / 5

CD8 Antibody (MHCD0804-4) in Flow

Dual color flow cytometry dot plot showing mouse anti human CD8 RPE vs. mouse anti human CD4 biotin followed by streptavidin RPE-Alexa Fluor® 610. Human mononuclear cells were stained with mouse anti human CD4 biotin (Product # MHCD0415), washed, stained with streptavidin RPE-Alexa Fluor® 610 (Product # S-20982) and mouse anti human CD8 RPE (Product # MHCD0804), washed, and analyzed on a flow cytometer with a 488 nm excitation source.

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Product Details

MHCD0804-4

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 11 publications 11 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3B5

Immunogen

Human CD8

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with sucrose, BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539726

Product Specific Information

R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

SARS-CoV-2 CD8⁺ T cell killing assays using replicating viruses and transgenic antigens.

STAR protocols

Mateyka LM,Grass V,Pichlmair A,Busch DH,D'Ippolito E

MHCD0804 was used in Flow cytometry/Cell sorting to we describe killing assays and determine the function of CD8+ T cells engineered with SARS-CoV-2-specific TCRs for antigen presentation.

Fri Dec 16 00:00:00 EST 2022

Recruitment of highly cytotoxic CD8⁺ T cell receptors in mild SARS-CoV-2 infection.

Cell reports

Wagner KI,Mateyka LM,Jarosch S,Grass V,Weber S,Schober K,Hammel M,Burrell T,Kalali B,Poppert H,Beyer H,Schambeck S,Holdenrieder S,Strötges-Achatz A,Haselmann V,Neumaier M,Erber J,Priller A,Yazici S,Roggendorf H,Odendahl M,Tonn T,Dick A,Witter K,Mijočević H,Protzer U,Knolle PA,Pichlmair A,Crowell CS,Gerhard M,D'Ippolito E,Busch DH

MHCD0804 was used in Flow Cytometry to isolate T cell receptor (TCR) repertoires specific for immunodominant SARS-CoV-2 epitopes restricted to common human Leukocyte antigen (HLA) class I molecules in convalescent individuals.

Tue Jan 11 00:00:00 EST 2022

Targeted T cell receptor gene editing provides predictable T cell product function for immunotherapy.

Cell reports. Medicine

Müller TR,Jarosch S,Hammel M,Leube J,Grassmann S,Bernard B,Effenberger M,Andrä I,Chaudhry MZ,Käuferle T,Malo A,Cicin-Sain L,Steinberger P,Feuchtinger T,Protzer U,Schumann K,Neuenhahn M,Schober K,Busch DH

MHCD0804 was used in Flow Cytometry to conclude that increased T cell product homogeneity after targeted TCR gene editing correlates with predictable in vivo T cell responses, which represents a crucial aspect for clinical application in adoptive T cell immunotherapy.

Tue Aug 17 00:00:00 EDT 2021

Blocking interaction between SHP2 and PD-1 denotes a novel opportunity for developing PD-1 inhibitors.

EMBO molecular medicine

Fan Z,Tian Y,Chen Z,Liu L,Zhou Q,He J,Coleman J,Dong C,Li N,Huang J,Xu C,Zhang Z,Gao S,Zhou P,Ding K,Chen L

MHCD0804 was used in Flow cytometry/Cell sorting to determine steps within PD-1 signalling pathway that can be targeted in anticancer drugs.

Mon Jun 08 00:00:00 EDT 2020

Interferon-α alters host glycosylation machinery during treated HIV infection.

EBioMedicine

Giron LB,Colomb F,Papasavvas E,Azzoni L,Yin X,Fair M,Anzurez A,Damra M,Mounzer K,Kostman JR,Tebas P,O'Doherty U,Tateno H,Liu Q,Betts MR,Montaner LJ,Abdel-Mohsen M

MHCD0804 was used in Flow cytometry/Cell sorting to identify significant interactions that support a model in which a) IFNα increases the proportion of pro-inflammatory, bisecting GlcNAc glycans (known to enhance FcγR binding) within the IgG glycome, which in turn b) increases inflammation, which c) leads to poor CD8+ T cell phenotypes and poor IFNα-mediated reduction of HIV DNA.

Tue Sep 01 00:00:00 EDT 2020

Simultaneous, Single-Cell Measurement of Messenger RNA, Cell Surface Proteins, and Intracellular Proteins.

Current protocols in cytometry

Soh KT,Tario JD,Colligan S,Maguire O,Pan D,Minderman H,Wallace PK

Wed Jan 06 00:00:00 EST 2016

IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.

Leukemia

Pegram HJ,Purdon TJ,van Leeuwen DG,Curran KJ,Giralt SA,Barker JN,Brentjens RJ

MHCD0804 was used in flow cytometry to developed a strategy to expand umbilical cord blood T cells and test their effects after transplantation

Sun Feb 01 00:00:00 EST 2015

Cross-presentation of viral antigens in dribbles leads to efficient activation of virus-specific human memory T cells.

Journal of translational medicine

Ye W,Xing Y,Paustian C,van de Ven R,Moudgil T,Hilton TL,Fox BA,Urba WJ,Zhao W,Hu HM

MHCD0804 was used in flow cytometry to study virus-specific human memory T-cell activation in response to Dribble-mediated cross-presentation of viral antigens.

Wed Apr 16 00:00:00 EDT 2014

Cancer regression and neurological toxicity following anti-MAGE-A3 TCR gene therapy.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Morgan RA,Chinnasamy N,Abate-Daga D,Gros A,Robbins PF,Zheng Z,Dudley ME,Feldman SA,Yang JC,Sherry RM,Phan GQ,Hughes MS,Kammula US,Miller AD,Hessman CJ,Stewart AA,Restifo NP,Quezado MM,Alimchandani M,Rosenberg AZ,Nath A,Wang T,Bielekova B,Wuest SC,Akula N,McMahon FJ,Wilde S,Mosetter B,Schendel DJ,Laurencot CM,Rosenberg SA

MHCD0804 was used in flow cytometry to report the detrimental effects observed in patients treated with autologous anti-MAGE-A3 T-cell receptor-engineered T cells

Fri Feb 01 00:00:00 EST 2013

Identification of a NCR+/NKG2D+/LFA-1(low)/CD94(-) immature human NK cell subset.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Zamai L,Galeotti L,Del Zotto G,Canonico B,Mirandola P,Papa S

MHCD08044 was used in flow cytometry to examine the effects of flt3 ligand and IL-15 on CD56(bright) natural killer cells

Sun Nov 01 00:00:00 EDT 2009

Flow cytometry in the bone marrow staging of mature B-cell neoplasms.

Cytometry. Part B, Clinical cytometry

Stacchini A,Demurtas A,Godio L,Martini G,Antinoro V,Palestro G

MHCD0804 was used in flow cytometry to ask if flow cytometry can be used for the detection of bone marrow involvement in the different types of B-cell malignant neoplasms.

Tue Jul 01 00:00:00 EDT 2003

Regulation of thymic epithelium by keratinocyte growth factor.

Blood

Erickson M,Morkowski S,Lehar S,Gillard G,Beers C,Dooley J,Rubin JS,Rudensky A,Farr AG

MHCD0804 was used in flow cytometry to elucidate how keratinocyte growth factor and FGFR2IIIb signaling affect the development and function of thymic epithelium

Fri Nov 01 00:00:00 EST 2002

Reactive oxygen intermediates during programmed cell death induced in the thymus of the Ts(1716)65Dn mouse, a murine model for human Down's syndrome.

Journal of immunology (Baltimore, Md. : 1950)

Paz-Miguel JE,Flores R,Sánchez-Velasco P,Ocejo-Vinyals G,Escribano de Diego J,López de Rego J,Leyva-Cobián F

MHCD0804 was used in flow cytometry to elucidate role of SOD-1 overexpression in thymocyte biology.

Mon Nov 15 00:00:00 EST 1999

Gammadelta+ T cells preferentially respond to live rather than killed malaria parasites.

Infection and immunity

Waterfall M,Black A,Riley E

MHCD0804 was used in flow cytometry to study responses of peripheral blood T cells to malaria antigens.

Fri May 01 00:00:00 EDT 1998

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8 Monoclonal Antibody (3B5), PE

CD8 Antibody (MHCD0804-4) in Flow

Product Details

MHCD0804-4

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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