Invitrogen

CD70 Monoclonal Antibody (FR70), Functional Grade, eBioscience™

FIGURE: 1 / 1

CD70 Antibody (16-0701-82) in Flow

Staining of 4-day LPS activated BALB/c splenocytes with 0.25 µg of Rat IgG2b kappa Isotype Control Functional Grade Purified (Product # 16-4031-81) (open histogram) or 0.25 µg of Anti-Mouse CD70 Functional Grade Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85)and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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Product Details

16-0701-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 4 publications 4 publications

Neutralization (Neu)

Assay-Dependent

Functional Assay (Functional)

Assay-Dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

FR70

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468997

Product Specific Information

Description: The FR70 monoclonal antibody reacts with mouse CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.

Applications Reported: The FR70 antibody has been reported for use in flow cytometric analysis. FR70 has also been reported in blocking of CD70 in vitro .

Applications Tested: The FR70 antibody has been tested by flow cytometric analysis of LPS-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Epitope-specific regulation of memory programming by differential duration of antigen presentation to influenza-specific CD8(+) T cells.

Immunity

Ballesteros-Tato A,León B,Lee BO,Lund FE,Randall TD

16-0701 was used in funtional assays to characterise the signals received by CD8 T cells responding to different epitopes of influenza virus during the primary response, and the effects on their secondary responsiveness.

Thu Jul 17 00:00:00 EDT 2014

Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells.

Nature immunology

Mahmud SA,Manlove LS,Schmitz HM,Xing Y,Wang Y,Owen DL,Schenkel JM,Boomer JS,Green JM,Yagita H,Chi H,Hogquist KA,Farrar MA

16-0701 was used in Flow cytometry/Cell sorting to study the role of TNF receptors in the thymic development of Treg cells..

Thu May 01 00:00:00 EDT 2014

Immune-based antitumor effects of BRAF inhibitors rely on signaling by CD40L and IFNγ.

Cancer research

Ho PC,Meeth KM,Tsui YC,Srivastava B,Bosenberg MW,Kaech SM

16-0701 was used in Flow cytometry/Cell sorting to investigate whether B-Raf(V600E) inhibitors promote tumor regression with the help of host immunity, showing that they rely on signaling by CD40L and IFNγ.

Sun Jun 15 00:00:00 EDT 2014

Lymphatic endothelial cells induce tolerance via PD-L1 and lack of costimulation leading to high-level PD-1 expression on CD8 T cells.

Blood

Tewalt EF,Cohen JN,Rouhani SJ,Guidi CJ,Qiao H,Fahl SP,Conaway MR,Bender TP,Tung KS,Vella AT,Adler AJ,Chen L,Engelhard VH

16-0701 was used in Flow cytometry/Cell sorting to demonstrate that LECs mediate deletion only via programmed cell death-1 (PD-1) ligand 1.

Thu Dec 06 00:00:00 EST 2012

CD70-driven costimulation induces survival or Fas-mediated apoptosis of T cells depending on antigenic load.

Journal of immunology (Baltimore, Md. : 1950)

Wensveen FM,Unger PP,Kragten NA,Derks IA,ten Brinke A,Arens R,van Lier RA,Eldering E,van Gisbergen KP

16-0701 was used in Flow cytometry/Cell sorting to investigate how BIm-dependent apoptosis is initiated in CD8 T cells.

Tue May 01 00:00:00 EDT 2012

Loss of T cell receptor-induced Bmi-1 in the KLRG1(+) senescent CD8(+) T lymphocyte.

Proceedings of the National Academy of Sciences of the United States of America

Heffner M,Fearon DT

16-0701 was used in Immunopurification to indicate that Bmi-1 is required for CD8(+) T cell clonal expansion and is positively regulated by receptors that mediate this response.

Tue Aug 14 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD27 ligand; CD27-L; CD70; CD70 antigen; Ki-24 antigen; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7

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Gene Aliases: Cd27l; CD27LG; Cd70; Tnfsf7

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UniProt ID: (Mouse) O55237

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Entrez Gene ID: (Mouse) 21948

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Function(s)

protease binding receptor binding cytokine activity tumor necrosis factor receptor binding

Process(es)

immune response signal transduction cell-cell signaling cell proliferation extrinsic apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD70 Monoclonal Antibody (FR70), Functional Grade, eBioscience™

CD70 Antibody (16-0701-82) in Flow

Product Details

16-0701-82

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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