Invitrogen

CD56 Monoclonal Antibody (123C3)

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

10 References

View all (202) CD56 (NCAM) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD56 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

CD56 Antibody (MA5-16446) in ICC/IF

Immunofluorescence analysis of Neural cell adhesion molecule 1 was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD56 Monoclonal Antibody (123C3) (Product # MA5-16445, MA5-16446) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000), for 45 m... View More

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CD56 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across the cell lines tested owing to their inherent genetic constitution. Relative expression of Neural cell adhesion molecule 1 was observed between SH-SY5Y, IMR-32, NK-92, SK-N-SH and breast cancer cell lines such as MCF7, SK-BR-3 and T-47D (DOI: 10.1038/s41598-019-45377-8) using Anti-CD56 Monoclonal Antibody (123C3) (Product # MA5-16445, MA5-16446) in Western Blot. {RE}

Product Details

MA5-16446

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:200

Flow Cytometry (Flow)

1:25-1:50

ELISA (ELISA)

Assay-dependent

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

123C3

Immunogen

Small cell lung carcinoma

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537961

Product Specific Information

Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD56 antibody, clone 123C3 recognizes human neural cell adhesion molecule (NCAM), otherwise known as CD56.

Target Information

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Usefulness of the octreotide test in Japanese patients for predicting the presence/absence of somatostatin receptor 2 expression in insulinomas.

Endocrine journal

Nakamura A,Mitsuhashi T,Takano Y,Miyoshi H,Kameda H,Nomoto H,Nagai S,Hatanaka Y,Shimizu C,Terauchi Y,Atsumi T

MA516446 was used in immunohistochemistry - paraffin section to assess the relationship between the results of the octreotide test and somatostatin receptor 2 expression in insulinoma patients to predict insulinomas in Japanese patients

Fri Dec 23 00:00:00 EST 2016

Role of cd56 and e-cadherin expression in the differential diagnosis of papillary thyroid carcinoma and suspected follicular-patterned lesions of the thyroid: the prognostic importance of e-cadherin.

International journal of clinical and experimental pathology

Ceyran AB,Şenol S,Şimşek BÇ,Sağıroğlu J,Aydın A

MA5-16446 was used in immunohistochemistry - paraffin section to discuss how to distinguish follicular variant of PTC from other follicular-patterned lesions of the thyroid.

Thu Mar 24 00:00:00 EDT 2016

Variable Expression of Neural Cell Adhesion Molecule Isoforms in Renal Tissue: Possible Role in Incipient Renal Fibrosis.

PloS one

Marković-Lipkovski J,Životić M,Müller CA,Tampe B,Ćirović S,Vještica J,Tomanović N,Zeisberg M,Müller GA

MA5-16446 was used in immunohistochemistry - paraffin section to learn the possible role of incipient renal fibrosis by variable expression of neural cell adhesion molecule isoforms in renal tissue

Wed May 18 00:00:00 EDT 2016

CD30⁺ extranodal natural killer/T-cell lymphoma mimicking phlegmonous myositis: A case report.

Oncology letters

Yang YJ,Li YX,Liu YB,Yang M,Liu K

MA5-16446 was used in immunohistochemistry (paraffin) to report on a case of CD30-positive extranodal natural killer/T-cell lymphoma.

Thu May 01 00:00:00 EDT 2014

A Case of Distal Epithelioid Sarcoma of the Thumb Expressing Podoplanin, TLE1 and Ca 125.

Case reports in pathology

Karagkounis G,Argyrakos T,Charkiolakis G,Castana O,Rontogianni D

MA5-16446 was used in immunohistochemistry to characterize the case of a distal epithelioid sarcoma arising in the thumb of a 14-year-old girl

Wed May 22 00:00:00 EDT 2013

Serous effusion cytology of extranodal natural killer/T-cell lymphoma.

Cytopathology : official journal of the British Society for Clinical Cytology

Su XY,Huang J,Jiang Y,Tang Y,Li GD,Liu WP

MA5-16446 was used in flow cytometry to characterize extranodal natural killer/T-cell lymphoma, nasal type using patient samples.

Sun Apr 01 00:00:00 EDT 2012

Subcutaneous panniculitis-like T-cell lymphoma: a clinical and pathologic study of 14 korean patients.

Annals of dermatology

Lee DW,Yang JH,Lee SM,Won CH,Chang S,Lee MW,Choi JH,Moon KC

MA5-16446 was used in immunohistochemistry to study subcutaneous panniculitis-like T-cell lymphomas in Korean patients

Mon Aug 01 00:00:00 EDT 2011

Extraosseous (extramedullary) plasmacytomas: a clinicopathologic and immunophenotypic study of 32 Chinese cases.

Diagnostic pathology

Zuo Z,Tang Y,Bi CF,Zhang WY,Zhao S,Wang XQ,Yang QP,Zou LQ,Liu WP

MA5-16446 was used in immunohistochemistry - paraffin section to determine the clinicopathologic features and diagnosis of extraosseous plasmacytomas

Mon Dec 19 00:00:00 EST 2011

Primary plasmacytoma of the cervix in a 21-year-old female patient.

International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists

Schor AP,Moraes MP,Bisson FW,Bisson MA,Luiz OM,Bacchi CE

MA5-16446 was used in immunohistochemistry to report on a case of primary plasmacytoma of the cervix

Sat May 01 00:00:00 EDT 2010

Goblet cell carcinoid of the appendix: a clinicopathological and immunohistochemical study of 26 cases from southwest china.

International journal of surgical pathology

MA5-16446 was used in immunohistochemistry - paraffin section to report the clinicopathological and immunohistochemical features of goblet cell carcinoid

Wed Dec 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: antigen recognized by monoclonal antibody 5.1H11; CD-56; CD56; E NCAM; N CAM1; N-CAM-1; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM

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Gene Aliases: CD56; MSK39; NCAM; NCAM1

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UniProt ID: (Human) P13591

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Entrez Gene ID: (Human) 4684

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Function(s)

virus receptor activity Ras guanyl-nucleotide exchange factor activity

Process(es)

MAPK cascade cell adhesion axon guidance positive regulation of GTPase activity viral entry into host cell regulation of synaptic plasticity interferon-gamma-mediated signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD56 Monoclonal Antibody (123C3)

CD56 Antibody (MA5-16446) in ICC/IF

Product Details

MA5-16446

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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