Invitrogen

CD45RO Monoclonal Antibody (UCHL1), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

3 Published Figures

12 References

View all (42) CD45RO antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD45RO Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD45RO Antibody (25-0457-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45RA APC (Product # 17-0458-42) and Mouse IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4724-81) (left) or Anti-Human CD45RO PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

25-0457-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

UCHL1

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10718534

Product Specific Information

Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.

Applications Reported: This UCHL1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This UCHL1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CD8⁺ T cells specific for an immunodominant SARS-CoV-2 nucleocapsid epitope display high naive precursor frequency and TCR promiscuity.

Immunity

Nguyen THO,Rowntree LC,Petersen J,Chua BY,Hensen L,Kedzierski L,van de Sandt CE,Chaurasia P,Tan HX,Habel JR,Zhang W,Allen LF,Earnest L,Mak KY,Juno JA,Wragg K,Mordant FL,Amanat F,Krammer F,Mifsud NA,Doolan DL,Flanagan KL,Sonda S,Kaur J,Wakim LM,Westall GP,James F,Mouhtouris E,Gordon CL,Holmes NE,Smibert OC,Trubiano JA,Cheng AC,Harcourt P,Clifton P,Crawford JC,Thomas PG,Wheatley AK,Kent SJ,Rossjohn J,Torresi J,Kedzierska K

25-0457-42 was used in Flow Cytometry to demonstrate high naive precursor frequency and TCRαβ diversity within immunodominant B7/N105-specific CD8+ T cells and provide insight into SARS-CoV-2-specific T cell origins and subsequent responses.

Tue May 11 00:00:00 EDT 2021

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

25-0457-42 was used in Flow cytometry/Cell sorting to explore the expression of iNKT cells in psoriasis patients and the effect of secukinumab on them.

Wed Feb 23 00:00:00 EST 2022

Longitudinal analysis of subtype C envelope tropism for memory CD4⁺ T cell subsets over the first 3 years of untreated HIV-1 infection.

Retrovirology

Gartner MJ,Gorry PR,Tumpach C,Zhou J,Dantanarayana A,Chang JJ,Angelovich TA,Ellenberg P,Laumaea AE,Nonyane M,Moore PL,Lewin SR,Churchill MJ,Flynn JK,Roche M

Published figure using CD45RO monoclonal antibody (Product # 25-0457-42) in Flow Cytometry

Thu Aug 06 00:00:00 EDT 2020

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD45RO monoclonal antibody (Product # 25-0457-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD45RO monoclonal antibody (Product # 25-0457-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Single-Cell Approach to Influenza-Specific CD8⁺ T Cell Receptor Repertoires Across Different Age Groups, Tissues, and Following Influenza Virus Infection.

Frontiers in immunology

Sant S,Grzelak L,Wang Z,Pizzolla A,Koutsakos M,Crowe J,Loudovaris T,Mannering SI,Westall GP,Wakim LM,Rossjohn J,Gras S,Richards M,Xu J,Thomas PG,Loh L,Nguyen THO,Kedzierska K

25-0457 was used in Flow cytometry/Cell sorting to provide novel findings on influenza-specific TCRαΒ repertoires within human tissues, raises the question of how we can prevent the loss of optimal TCRαΒ signatures with aging, and provides important insights into the rational design of T cell-mediated vaccines and immunotherapies.

Wed Nov 20 00:00:00 EST 2019

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD45RO monoclonal antibody (Product # 25-0457-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Selective blockade of CD28 on human T cells facilitates regulation of alloimmune responses.

JCI insight

Zaitsu M,Issa F,Hester J,Vanhove B,Wood KJ

25-0457 was used in Flow cytometry/Cell sorting to show that a nonactivating monovalent anti-CD28 that spares CTLA4 signaling is an effective immunosuppressant in a clinically relevant humanized mouse transplant model.

Thu Oct 05 00:00:00 EDT 2017

Modeling the dynamics of neonatal CD8⁺ T-cell responses.

Immunology and cell biology

Reynaldi A,Smith NL,Schlub TE,Venturi V,Rudd BD,Davenport MP

25-0457 was used in Flow cytometry/Cell sorting to analyse the dynamics of neonatal and adult CD8 T cell responses following in vitro stimulation and in vivo infection.

Sat Oct 01 00:00:00 EDT 2016

Promoter H3K4 methylation dynamically reinforces activation-induced pathways in human CD4 T cells.

Genes and immunity

LaMere SA,Thompson RC,Komori HK,Mark A,Salomon DR

25-0457 was used in Flow cytometry/Cell sorting to examine genome-wide histone modification dynamics during the activation of human naive and memory CD4 T cells.

Fri Jul 01 00:00:00 EDT 2016

Functional expression of CD137 (4-1BB) on T helper follicular cells.

Oncoimmunology

Alfaro C,Echeveste JI,Rodriguez-Ruiz ME,Solorzano JL,Perez-Gracia JL,Idoate MA,Lopez-Picazo JM,Sanchez-Paulete AR,Labiano S,Rouzaut A,Oñate C,Aznar A,Lozano MD,Melero I

Published figure using CD45RO monoclonal antibody (Product # 25-0457-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Differential expression of CD300a/c on human TH1 and TH17 cells.

BMC immunology

Simhadri VR,Mariano JL,Zhou Q,DeBell KE,Borrego F

25-0457 was used in Flow cytometry/Cell sorting to conclude that the CD300a/c receptors are differentially expressed on human TH1 and TH17 cells, and that their ligation is capable of modulating TCR mediated signals.

Wed Nov 02 00:00:00 EDT 2011

Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.

PloS one

Narayanan S,Silva R,Peruzzi G,Alvarez Y,Simhadri VR,Debell K,Coligan JE,Borrego F

25-0457 was used in Flow cytometry/Cell sorting to investigate a subset within the CD4 T cell population defined by expression of the immunomodulatory receptor CD300a.

Thu May 13 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45RO Monoclonal Antibody (UCHL1), PE-Cyanine7, eBioscience™

CD45RO Antibody (25-0457-42) in Flow

Product Details

25-0457-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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