Invitrogen

CD40 Monoclonal Antibody (5C3), Functional Grade, eBioscience™

FIGURE: 1 / 12

CD40 Antibody (16-0409-81) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD40 FITC (left) or PE (right).Appropriate isotype controls were used (open histogram).Cells in the lymphocyte population were used for analysis.

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Product Details

16-0409-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 13 publications 13 publications

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

5C3

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468948

Product Specific Information

Description: The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays.

Applications Reported: The 5C3 antibody has been reported for use in flow cytometric analysis. 5C3 has also been reported in in vitro functional studies.

Applications Tested: The 5C3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Recombinant human IL-37 inhibited endometriosis development in a mouse model through increasing Th1/Th2 ratio by inducing the maturation of dendritic cells.

Reproductive biology and endocrinology : RB&E

Li L,Liao Z,Ye M,Jiang J

Published figure using CD40 monoclonal antibody (Product # 16-0409-81) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Activated monocytes and markers of inflammation in newly diagnosed multiple sclerosis.

Immunology and cell biology

Carstensen M,Christensen T,Stilund M,Møller HJ,Petersen EL,Petersen T

Published figure using CD40 monoclonal antibody (Product # 16-0409-81) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

A replication-incompetent CD154/40L recombinant vaccinia virus induces direct and macrophage-mediated antitumor effects in vitro and in vivo.

Oncoimmunology

Governa V,Brittoli A,Mele V,Pinamonti M,Terracciano L,Muenst S,Iezzi G,Spagnoli GC,Zajac P,Trella E

16-0409-81 was used in Flow Cytometry to observe that intra-tumoral injection of rVV40L-infected human macrophages inhibits progression of human CD40(-) tumors in vivo.

Tue Jan 12 00:00:00 EST 2021

Transitional B Cells and TLR9 Responses Are Defective in Selective IgA Deficiency.

Frontiers in immunology

Lemarquis AL,Einarsdottir HK,Kristjansdottir RN,Jonsdottir I,Ludviksson BR

16-0409 was used in Flow cytometry/Cell sorting to analyse the phenotype and function of T and B cells in individuals with IgA deficiency following IgA-inducing CpG-TLR9 stimulations.

Mon Jun 24 00:00:00 EDT 2019

Subsets of activated monocytes and markers of inflammation in incipient and progressed multiple sclerosis.

Immunology and cell biology

Gjelstrup MC,Stilund M,Petersen T,Møller HJ,Petersen EL,Christensen T

Published figure using CD40 monoclonal antibody (Product # 16-0409-81) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.

Stem cell research & therapy

Ventura Ferreira MS,Bienert M,Müller K,Rath B,Goecke T,Opländer C,Braunschweig T,Mela P,Brümmendorf TH,Beier F,Neuss S

Published figure using CD40 monoclonal antibody (Product # 16-0409-81) in Flow Cytometry

Mon Feb 05 00:00:00 EST 2018

An Immune Atlas of Clear Cell Renal Cell Carcinoma.

Cell

Chevrier S,Levine JH,Zanotelli VRT,Silina K,Schulz D,Bacac M,Ries CH,Ailles L,Jewett MAS,Moch H,van den Broek M,Beisel C,Stadler MB,Gedye C,Reis B,Pe'er D,Bodenmiller B

Published figure using CD40 monoclonal antibody (Product # 16-0409-81) in Flow Cytometry

Thu May 04 00:00:00 EDT 2017

Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.

Oncotarget

Pathangey LB,McCurry DB,Gendler SJ,Dominguez AL,Gorman JE,Pathangey G,Mihalik LA,Dang Y,Disis ML,Cohen PA

Published figure using CD40 monoclonal antibody (Product # 16-0409-81) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2017

CD16⁺ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.

Frontiers in immunology

Zhu H,Hu F,Sun X,Zhang X,Zhu L,Liu X,Li X,Xu L,Shi L,Gan Y,Su Y

16-0409 was used in Flow cytometry/Cell sorting to demonstrate that CD16+monocytes were enriched and played a critical role in driving the pathogenic T- and B-cell responses in patients with systemic lupus erythematosus.

Wed Nov 20 00:00:00 EST 2019

A simple, accurate and universal method for quantification of PCR.

BMC biotechnology

Boulter N,Suarez FG,Schibeci S,Sunderland T,Tolhurst O,Hunter T,Hodge G,Handelsman D,Simanainen U,Hendriks E,Duggan K

Published figure using CD40 monoclonal antibody (Product # 16-0409-81) in Flow Cytometry

Wed Mar 09 00:00:00 EST 2016

Transcriptional and functional characterization of CD137L-dendritic cells identifies a novel dendritic cell phenotype.

Scientific reports

Harfuddin Z,Dharmadhikari B,Wong SC,Duan K,Poidinger M,Kwajah S,Schwarz H

16-0409 was used in Functional assays to characterise the gene expression profile of CD137 ligand (CD137L)-dendritic cells (DCs), and identify significant similarities of CD137L-DCs with in vivo inflammatory monocyte-derived DCs and macrophages.

Tue Jul 19 00:00:00 EDT 2016

A novel subset of B7-H3⁺CD14⁺HLA-DR^-/low myeloid-derived suppressor cells are associated with progression of human NSCLC.

Oncoimmunology

Zhang G,Huang H,Zhu Y,Yu G,Gao X,Xu Y,Liu C,Hou J,Zhang X

Published figure using CD40 monoclonal antibody (Product # 16-0409-81) in Flow Cytometry

Sun Feb 01 00:00:00 EST 2015

Direct tumor recognition by a human CD4(+) T-cell subset potently mediates tumor growth inhibition and orchestrates anti-tumor immune responses.

Scientific reports

Matsuzaki J,Tsuji T,Luescher IF,Shiku H,Mineno J,Okamoto S,Old LJ,Shrikant P,Gnjatic S,Odunsi K

16-0409 was used in Blocking experiments to provide mechanistic insights into the role of tumour-recognising CD4(+) T cells in tumour immunity.

Thu Oct 08 00:00:00 EDT 2015

A CD8 T cell/indoleamine 2,3-dioxygenase axis is required for mesenchymal stem cell suppression of human systemic lupus erythematosus.

Arthritis & rheumatology (Hoboken, N.J.)

Wang D,Feng X,Lu L,Konkel JE,Zhang H,Chen Z,Li X,Gao X,Lu L,Shi S,Chen W,Sun L

16-0409 was used in Functional assays to highlight a previously unrecognised CD8+ T cell/IFNγ/IDO axis that mediates the therapeutic effects of allogeneic MSCs in lupus patients.

Fri Aug 01 00:00:00 EDT 2014

Endothelial microparticles interact with and support the proliferation of T cells.

Journal of immunology (Baltimore, Md. : 1950)

Wheway J,Latham SL,Combes V,Grau GE

16-0409 was used in Flow cytometry/Cell sorting to explore the effect of endothelial cell microparticles in T cell activation and antigen presentation.

Wed Oct 01 00:00:00 EDT 2014

Severe loss of invariant NKT cells exhibiting anti-HTLV-1 activity in patients with HTLV-1-associated disorders.

Blood

Azakami K,Sato T,Araya N,Utsunomiya A,Kubota R,Suzuki K,Hasegawa D,Izumi T,Fujita H,Aratani S,Fujii R,Yagishita N,Kamijuku H,Kanekura T,Seino K,Nishioka K,Nakajima T,Yamano Y

16-0409 was used in Flow cytometry/Cell sorting to assess the contribution of iNKT cells to the immune defence against human T-lymphotropic virus type 1 and associated disorders.

Thu Oct 08 00:00:00 EDT 2009

CD46-induced human Treg enhance B-cell responses.

European journal of immunology

Fuchs A,Atkinson JP,Fremeaux-Bacchi V,Kemper C

16-0409 was used in Functional assays to analyse the effect of cTreg on B-cell functions in a co-culture system.

Sun Nov 01 00:00:00 EDT 2009

Airway epithelial cells produce B cell-activating factor of TNF family by an IFN-beta-dependent mechanism.

Journal of immunology (Baltimore, Md. : 1950)

Kato A,Truong-Tran AQ,Scott AL,Matsumoto K,Schleimer RP

16-0409 was used in an Enzyme Immunoassay to investigate a novel mechanism for BAFF family expression.

Wed Nov 15 00:00:00 EST 2006

Bioinformatics

Protein Aliases: B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDw40; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40; Tumor necrosis factor receptor superfamily member 5

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Gene Aliases: Bp50; CD40; CDW40; p50; TNFRSF5

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UniProt ID: (Human) P25942

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Entrez Gene ID: (Human) 958

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Function(s)

antigen binding signal transducer activity receptor activity tumor necrosis factor-activated receptor activity protein binding enzyme binding ubiquitin protein ligase binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD40 Monoclonal Antibody (5C3), Functional Grade, eBioscience™

CD40 Antibody (16-0409-81) in Flow

Product Details

16-0409-81

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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