Invitrogen

CD366 (TIM3) Monoclonal Antibody (F38-2E2), APC, eBioscience™

CD366 (TIM3) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD366 (TIM3) Antibody (17-3109-42) in Flow

Staining of unstimulated (left) or Anti-Human CD3 and Anti-Human CD28 Functional Grade Purified (Product # 16-0037-81 and Product # 16-0289-81)-stimulated (right) normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0048-42) and Anti-Human CD366 (TIM3) APC. Total viable cells, as determined by Fixable Viability Dye eFluor® 780 (Product # 65-0865-14), were used for analysis.

Product Image Gallery

Product Details

17-3109-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

F38-2E2

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1963622

Product Specific Information

Description: This F38-2E2 monoclonal antibody reacts with human CD366, also known as T cell immunoglobulin and mucin domain-containing protein 3 (TIM3) or HAVCR2. This cell surface receptor is expressed on activated CD4+ T cell subsets (e.g. Th1, Th17, and Treg), CD8+ T cells, monocytes, dendritic cells, and mast cells. Due to alternative splicing, CD366 exists as membrane-bound and soluble forms. Galectin-9 has been identified as the ligand for CD366. In humans, this receptor negatively regulates CD4+ T cells, influencing the secretion of some Th1- and Th17-related cytokines. CD366 has also been implicated in tolerance, autoimmune disease (e.g. multiple sclerosis), and HIV infection.

Applications Reported: This F38-2E2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This F38-2E2 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

MYEF2: an immune infiltration-related prognostic factor in IDH-wild-type glioblastoma.

Aging

Zhang Y,Wen Y,Nie J,Wang T,Wang G,Gao Q,Cao Y,Wang H,Qi S,Xie S

Published figure using CD366 (TIM3) monoclonal antibody (Product # 17-3109-42) in Flow Cytometry

Tue Aug 08 00:00:00 EDT 2023

Neoadjuvant chemotherapy plus nivolumab with or without ipilimumab in operable non-small cell lung cancer: the phase 2 platform NEOSTAR trial.

Nature medicine

Cascone T,Leung CH,Weissferdt A,Pataer A,Carter BW,Godoy MCB,Feldman H,William WN,Xi Y,Basu S,Sun JJ,Yadav SS,Rojas Alvarez FR,Lee Y,Mishra AK,Chen L,Pradhan M,Guo H,Sinjab A,Zhou N,Negrao MV,Le X,Gay CM,Tsao AS,Byers LA,Altan M,Glisson BS,Fossella FV,Elamin YY,Blumenschein G,Zhang J,Skoulidis F,Wu J,Mehran RJ,Rice DC,Walsh GL,Hofstetter WL,Rajaram R,Antonoff MB,Fujimoto J,Solis LM,Parra ER,Haymaker C,Wistuba II,Swisher SG,Vaporciyan AA,Lin HY,Wang J,Gibbons DL,Jack Lee J,Ajami NJ,Wargo JA,Allison JP,Sharma P,Kadara H,Heymach JV,Sepesi B

17-3109-42 was used in Flow cytometry/Cell sorting to report the results and correlates of two arms of the phase 2 platform NEOSTAR trial testing neoadjuvant Nivo+CT and Ipi+Nivo+CT with major pathologic response (MPR) as the primary endpoint.

Wed Mar 01 00:00:00 EST 2023

Expanding anti-CD38 immunotherapy for lymphoid malignancies.

Journal of experimental & clinical cancer research : CR

Wang X,Yu X,Li W,Neeli P,Liu M,Li L,Zhang M,Fang X,Young KH,Li Y

17-3109-42 was used in Flow cytometry/Cell sorting to expand anti-CD38 immunotherapy to a broad spectrum of lymphoid malignancies and call for the incorporation of ATRA into daratumumab or other anti-CD38 immunological agents for cancer therapy.

Tue Jun 28 00:00:00 EDT 2022

CRISPR Screening of CAR T Cells and Cancer Stem Cells Reveals Critical Dependencies for Cell-Based Therapies.

Cancer discovery

Wang D,Prager BC,Gimple RC,Aguilar B,Alizadeh D,Tang H,Lv D,Starr R,Brito A,Wu Q,Kim LJY,Qiu Z,Lin P,Lorenzini MH,Badie B,Forman SJ,Xie Q,Brown CE,Rich JN

17-3109-42 was used in Flow Cytometry to interrogate molecular determinants of CAR-mediated GBM killing through whole-genome CRISPR screens in both CAR T cells and patient-derived GSCs.

Sat May 01 00:00:00 EDT 2021

TIGIT⁺ TIM-3⁺ NK cells are correlated with NK cell exhaustion and disease progression in patients with hepatitis B virus‑related hepatocellular carcinoma.

Oncoimmunology

Yu L,Liu X,Wang X,Yan F,Wang P,Jiang Y,Du J,Yang Z

Published figure using CD366 (TIM3) monoclonal antibody (Product # 17-3109-42) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

SLC1A1 mediated glutamine addiction and contributed to natural killer T-cell lymphoma progression with immunotherapeutic potential.

EBioMedicine

Xiong J,Wang N,Zhong HJ,Cui BW,Cheng S,Sun R,Chen JY,Xu PP,Cai G,Wang L,Sun XJ,Huang JY,Zhao WL

Published figure using CD366 (TIM3) monoclonal antibody (Product # 17-3109-42) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition.

Frontiers in immunology

Yeku OO,Rao TD,Laster I,Kononenko A,Purdon TJ,Wang P,Cui Z,Liu H,Brentjens RJ,Spriggs D

17-3109-42 was used in Flow Cytometry to show the feasibility and efficacy of human MUC16 ectodomain- specific BiTEDs and provide a basis for the combination with anti-VEGF therapy for ovarian cancer.

Thu Oct 21 00:00:00 EDT 2021

Combined p14ARF and Interferon-β Gene Transfer to the Human Melanoma Cell Line SK-MEL-147 Promotes Oncolysis and Immune Activation.

Frontiers in immunology

Cerqueira OLD,Clavijo-Salomon MA,Cardoso EC,Citrangulo Tortelli Junior T,Mendonça SA,Barbuto JAM,Strauss BE

Published figure using CD366 (TIM3) monoclonal antibody (Product # 17-3109-42) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

Neutrophil expansion defines an immunoinhibitory peripheral and intratumoral inflammatory milieu in resected non-small cell lung cancer: a descriptive analysis of a prospectively immunoprofiled cohort.

Journal for immunotherapy of cancer

Mitchell KG,Diao L,Karpinets T,Negrao MV,Tran HT,Parra ER,Corsini EM,Reuben A,Federico L,Bernatchez C,Dejima H,Francisco-Cruz A,Wang J,Antonoff MB,Vaporciyan AA,Swisher SG,Cascone T,Wistuba II,Heymach JV,Gibbons DL,Zhang J,Haymaker CL,Sepesi B

17-3109 was used in Flow cytometry/Cell sorting to demonstrate that neutrophil expansion reflects protumorigenic and immunosuppressive processes that manifest as worse overall survival (OS) in patients undergoing non-small lung cancer (NSCLC) resection.

Wed Apr 01 00:00:00 EDT 2020

Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium.

PLoS pathogens

Pachnio A,Ciaurriz M,Begum J,Lal N,Zuo J,Beggs A,Moss P

17-3109 was used in Flow cytometry/Cell sorting to reveal the marked accumulation and unique phenotype of CMV-specific CD4+ T cells.

Thu Sep 01 00:00:00 EDT 2016

CEACAM1 mediates B cell aggregation in central nervous system autoimmunity.

Scientific reports

Rovituso DM,Scheffler L,Wunsch M,Kleinschnitz C,Dörck S,Ulzheimer J,Bayas A,Steinman L,Ergün S,Kuerten S

17-3109 was used in Flow cytometry/Cell sorting to explore the role of carcinogenic antigen-related cell adhesion molecule1 (CEACAM1) during disease-related B cell aggregation in MS patients.

Wed Jul 20 00:00:00 EDT 2016

Activation and propagation of tumor-infiltrating lymphocytes on clinical-grade designer artificial antigen-presenting cells for adoptive immunotherapy of melanoma.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Forget MA,Malu S,Liu H,Toth C,Maiti S,Kale C,Haymaker C,Bernatchez C,Huls H,Wang E,Marincola FM,Hwu P,Cooper LJ,Radvanyi LG

17-3109 was used in Flow cytometry/Cell sorting to compare the expansion, activation and differentiation of tumour-infiltrating lymphocytes in response to different developmental cues.

Thu Nov 12 00:00:00 EST 2015

Cis association of galectin-9 with Tim-3 differentially regulates IL-12/IL-23 expressions in monocytes via TLR signaling.

PloS one

Ma CJ,Li GY,Cheng YQ,Wang JM,Ying RS,Shi L,Wu XY,Niki T,Hirashima M,Li CF,Moorman JP,Yao ZQ

17-3109 was used in Flow cytometry/Cell sorting to study the interaction between Tim-3 expression in human monocytes/macrophages, its ligand Galectin, and the regulation of cytokine signalling.

Tue Mar 18 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD366; FLJ14428; HAVcr-2; Hepatitis A virus cellular receptor 2; kidney injury molecule-3; sCD366; soluble CD366; soluble TIM 3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM-3; TIMD-3

View more View less

Gene Aliases: CD366; HAVcr-2; HAVCR2; KIM-3; Tim-3; TIM3; TIMD-3; TIMD3

View more View less

UniProt ID: (Human) Q8TDQ0

View more View less

Entrez Gene ID: (Human) 84868

View more View less

Function(s)

protein binding metal ion binding immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD366 (TIM3) Monoclonal Antibody (F38-2E2), APC, eBioscience™

CD366 (TIM3) Antibody (17-3109-42) in Flow

Product Details

17-3109-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!