Invitrogen

CD3 Monoclonal Antibody (7D6), PE-Texas Red

5 Published Figures

13 References

View all (504) CD3 antibodies

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CD3 Antibody (MHCD0317) in Flow

Human peripheral blood lymphocytes stained using PE-Texas Red® of anti-human CD3 monoclonal antibody (clone S4.1). The negative control profiles represent unstained cells.

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Product Details

MHCD0317

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 11 publications 11 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

7D6

Immunogen

Human CD3 epsilon

Conjugate

PE-Texas Red PE-Texas Red PE-Texas Red

Excitation/Emission Max

567/615 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with BSA, sucrose

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10376002

Product Specific Information

The R-phycoerythrin (PE)-Texas Red conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

Analyte Specific Reagent

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Product References

Protein/AS01_B vaccination elicits stronger, more Th2-skewed antigen-specific human T follicular helper cell responses than heterologous viral vectors.

Cell reports. Medicine

Nielsen CM,Ogbe A,Pedroza-Pacheco I,Doeleman SE,Chen Y,Silk SE,Barrett JR,Elias SC,Miura K,Diouf A,Bardelli M,Dabbs RA,Barfod L,Long CA,Haynes BF,Payne RO,Minassian AM,Bradley T,Draper SJ,Borrow P

MHCD0317 was used in Flow Cytometry to highlight the impact of vaccine platform on the cTfh cell response driving humoral immunity, associating a high-magnitude, Th2-biased cTfh response with potent antibody production.

Tue Mar 16 00:00:00 EDT 2021

RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic Signatures.

ImmunoHorizons

Li D,Bradley T,Cain DW,Pedroza-Pacheco I,Aggelakopoulou M,Parks R,Barr M,Xia SM,Scearce R,Bowman C,Stevens G,Newman A,Hora B,Chen Y,Riebe K,Wang Y,Sempowski G,Saunders KO,Borrow P,Haynes BF

MHCD0317 was used in Flow cytometry/Cell sorting to study the role of Rab11Fip5 in immune responses.

Tue Nov 10 00:00:00 EST 2020

Transcriptomic Profiling of the Tumor Microenvironment Reveals Distinct Subgroups of Clear Cell Renal Cell Cancer: Data from a Randomized Phase III Trial.

Cancer discovery

Hakimi AA,Voss MH,Kuo F,Sanchez A,Liu M,Nixon BG,Vuong L,Ostrovnaya I,Chen YB,Reuter V,Riaz N,Cheng Y,Patel P,Marker M,Reising A,Li MO,Chan TA,Motzer RJ

MHCD0317 was used in Flow cytometry/Cell sorting to identify predictors of metastatic clear cell RCC (ccRCC) patient response to treatment with tyrosine kinase inhibitor therapy.

Mon Apr 01 00:00:00 EDT 2019

RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses.

Cell

Bradley T,Peppa D,Pedroza-Pacheco I,Li D,Cain DW,Henao R,Venkat V,Hora B,Chen Y,Vandergrift NA,Overman RG,Edwards RW,Woods CW,Tomaras GD,Ferrari G,Ginsburg GS,Connors M,Cohen MS,Moody MA,Borrow P,Haynes BF

Published figure using CD3d monoclonal antibody (Product # MHCD0317) in Flow Cytometry

Thu Oct 04 00:00:00 EDT 2018

Application of area scaling analysis to identify natural killer cell and monocyte involvement in the GranToxiLux antibody dependent cell-mediated cytotoxicity assay.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Pollara J,Orlandi C,Beck C,Edwards RW,Hu Y,Liu S,Wang S,Koup RA,Denny TN,Lu S,Tomaras GD,DeVico A,Lewis GK,Ferrari G

MHCD0317 was used in Flow cytometry/Cell sorting to show ASA use in ADCC-GTL assays provides additional assessment of the ability of natural and vaccine-induced antibodies to recruit NK cells.

Sun Apr 01 00:00:00 EDT 2018

Tumor immune microenvironment characterization in clear cell renal cell carcinoma identifies prognostic and immunotherapeutically relevant messenger RNA signatures.

Genome biology

Şenbabaoğlu Y,Gejman RS,Winer AG,Liu M,Van Allen EM,de Velasco G,Miao D,Ostrovnaya I,Drill E,Luna A,Weinhold N,Lee W,Manley BJ,Khalil DN,Kaffenberger SD,Chen Y,Danilova L,Voss MH,Coleman JA,Russo P,Reuter VE,Chan TA,Cheng EH,Scheinberg DA,Li MO,Choueiri TK,Hsieh JJ,Sander C,Hakimi AA

MHCD0317 was used in flow cytometry to profile the infiltration levels of immune cell populations in 19 cancer types

Thu Nov 17 00:00:00 EST 2016

Defining human dendritic cell progenitors by multiparametric flow cytometry.

Nature protocols

Breton G,Lee J,Liu K,Nussenzweig MC

MHCD0317 was used in Flow Cytometry to assess the in vitro differentiation and proliferation of DC progenitors.

Tue Sep 01 00:00:00 EDT 2015

Genetic and epigenetic variation in the lineage specification of regulatory T cells.

eLife

Arvey A,van der Veeken J,Plitas G,Rich SS,Concannon P,Rudensky AY

MHCD0317 was used in flow cytometry to study factors that specify the Treg lineage

Wed Oct 28 00:00:00 EDT 2015

Pre-existing hypertension dominates γδT cell reduction in human ischemic stroke.

PloS one

Adamski MG,Li Y,Wagner E,Yu H,Seales-Bailey C,Durkin H,Hao Q,Soper SA,Murphy M,Baird AE

Published figure using CD3d monoclonal antibody (Product # MHCD0317) in Flow Cytometry

Fri Jan 09 00:00:00 EST 2015

Monitoring of regulatory T cell frequencies and expression of CTLA-4 on T cells, before and after DC vaccination, can predict survival in GBM patients.

PloS one

Fong B,Jin R,Wang X,Safaee M,Lisiero DN,Yang I,Li G,Liau LM,Prins RM

MHCD0317 was used in flow cytometry to identify surrogates of anti-tumor immune responsiveness after DC vaccination.

Fri Jul 27 00:00:00 EDT 2012

Systemic effector and regulatory immune responses to chlamydial antigens in trachomatous trichiasis.

Frontiers in microbiology

Gall A,Horowitz A,Joof H,Natividad A,Tetteh K,Riley E,Bailey RL,Mabey DC,Holland MJ

MHCD0317 was used in flow cytometry to assess the immune responses of peripheral blood to chlamydial antigens.

Tue Oct 02 00:00:00 EDT 2012

NK cells as effectors of acquired immune responses: effector CD4+ T cell-dependent activation of NK cells following vaccination.

Journal of immunology (Baltimore, Md. : 1950)

Horowitz A,Behrens RH,Okell L,Fooks AR,Riley EM

MHCD0317 was used in flow cytometry to study the molecular mechanisms that regulate the "recall" NK cell response after rabies vaccination

Wed Sep 01 00:00:00 EDT 2010

Peripheral immature CD2-/low T cell development from type 2 to type 1 cytokine production.

Journal of immunology (Baltimore, Md. : 1950)

Loza MJ,Perussia B

MHCD0317 was used in flow cytometry to identify and characterize immature CD2(-/low) T cells in peripheral blood.

Sun Sep 15 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

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CD3 Monoclonal Antibody (7D6), PE-Texas Red

CD3 Antibody (MHCD0317) in Flow

Product Details

MHCD0317

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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