Invitrogen

CD278 (ICOS) Monoclonal Antibody (7E.17G9), PE-Cyanine5, eBioscience™

CD278 (ICOS) Antibody in Flow Cytometry (Flow)

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CD278 (ICOS) Antibody (15-9942-82) in Flow

Staining of unstimulated (left) and 3-day Con A-stimulated (right) BALB/c splenocytes with 0.06 µg of Rat IgG2b K Isotype Control PE-Cyanine5 (Product # 15-4031-82) (blue histogram) or 0.06 µg of Anti-Mouse CD278 (ICOS) PE-Cyanine5 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 660, were used for analysis.

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Product Details

15-9942-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

7E.17G9

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468826

Product Specific Information

Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.

Applications Reported: 7E.17G9 has been reported for use in flow cytometric analysis.

Applications Tested: The 7E.17G9 antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD278 (ICOS) monoclonal antibody (Product # 15-9942-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

MALT1 Proteolytic Activity Suppresses Autoimmunity in a T Cell Intrinsic Manner.

Frontiers in immunology

Demeyer A,Skordos I,Driege Y,Kreike M,Hochepied T,Baens M,Staal J,Beyaert R

15-9942 was used in Flow cytometry/Cell sorting to establish that suppression of autoimmune responses is due to the T-cell intrinsic role of MALT1 proteolytic activity.

Mon Oct 05 00:00:00 EDT 2020

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using CD278 (ICOS) monoclonal antibody (Product # 15-9942-82) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

Control of T_reg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells.

Nature communications

Chen S,Fang L,Guo W,Zhou Y,Yu G,Li W,Dong K,Liu J,Luo Y,Wang B,Li Z,Zhao C,Sun Z,Shen Y,Leng Q,Zhou D,Han Z,Huang H,Ren H,Xu G,Feng X

Published figure using CD278 (ICOS) monoclonal antibody (Product # 15-9942-82) in Flow Cytometry

Thu Dec 13 00:00:00 EST 2018

Roquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation.

Nature communications

Essig K,Kronbeck N,Guimaraes JC,Lohs C,Schlundt A,Hoffmann A,Behrens G,Brenner S,Kowalska J,Lopez-Rodriguez C,Jemielity J,Holtmann H,Reiche K,Hackermüller J,Sattler M,Zavolan M,Heissmeyer V

Published figure using CD278 (ICOS) monoclonal antibody (Product # 15-9942-82) in Flow Cytometry

Wed Sep 19 00:00:00 EDT 2018

Regulatory T Cells Promote Myositis and Muscle Damage in Toxoplasma gondii Infection.

Journal of immunology (Baltimore, Md. : 1950)

Jin RM,Blair SJ,Warunek J,Heffner RR,Blader IJ,Wohlfert EA

15-9942 was used in Flow cytometry/Cell sorting to study how the tissue environment established by Toxoplasma gondii leads to a pathogenic role for regulatory T cells.

Sun Jan 01 00:00:00 EST 2017

Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment.

Nature communications

Kim KD,Bae S,Capece T,Nedelkovska H,de Rubio RG,Smrcka AV,Jun CD,Jung W,Park B,Kim TI,Kim M

Published figure using CD278 (ICOS) monoclonal antibody (Product # 15-9942-82) in Flow Cytometry

Mon May 15 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 15-9942-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 15-9942-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

A Mouse Model of West Nile Virus Infection.

Current protocols in mouse biology

Graham JB,Swarts JL,Lund JM

15-9942 was used in Flow cytometry/Cell sorting to develop a mouse model to study the breadth of symptoms and disease severity seen in human West Nile virus infection.

Wed Dec 20 00:00:00 EST 2017

PD-L1hi B cells are critical regulators of humoral immunity.

Nature communications

Khan AR,Hams E,Floudas A,Sparwasser T,Weaver CT,Fallon PG

15-9942 was used in Flow cytometry/Cell sorting to demonstrate that elevated PD-L1 expression on B cells is an important regulator of T follicular helper-cell activity, downstream antibody responses and autoimmune disease.

Thu Jan 22 00:00:00 EST 2015

Blockade of B7-H1 (programmed death ligand 1) enhances humoral immunity by positively regulating the generation of T follicular helper cells.

Journal of immunology (Baltimore, Md. : 1950)

Hams E,McCarron MJ,Amu S,Yagita H,Azuma M,Chen L,Fallon PG

15-9942 was used in Flow cytometry/Cell sorting to demonstrate that the coinhibitory B7-H1/programmed death 1 pathway can limit the expansion of T(FH) cells and constrain Ag-specific Ig responses.

Sun May 15 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: Activation-inducible lymphocyte immunomediatory molecule; CCLP; CD278; CD28 and CTLA-4-like protein; CD28-related protein 1; CRP-1; inducible T-cell co-stimulator; Inducible T-cell costimulator

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Gene Aliases: AILIM; CCLP; CRP-1; H4; Icos; Ly115

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UniProt ID: (Mouse) Q9WVS0

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Entrez Gene ID: (Mouse) 54167

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Function(s)

protein binding

Process(es)

T cell tolerance induction single organismal cell-cell adhesion T cell costimulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD278 (ICOS) Monoclonal Antibody (7E.17G9), PE-Cyanine5, eBioscience™

CD278 (ICOS) Antibody (15-9942-82) in Flow

Product Details

15-9942-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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