Invitrogen

CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), PerCP-eFluor™ 710, eBioscience™

CD274 (PD-L1, B7-H1) Antibody in Flow Cytometry (Flow)

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CD274 (PD-L1, B7-H1) Antibody (46-5982-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.06 µg of Rat IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4321-82) (left) or 0.06 µg of Anti-Mouse CD274 (B7-H1) PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

46-5982-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 34 publications 34 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, lambda

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MIH5

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573819

Product Specific Information

Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.

Applications Reported: This MIH5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

AR-A014418 regulates intronic polyadenylation and transcription of PD-L1 through inhibiting CDK12 and CDK13 in tumor cells.

Journal for immunotherapy of cancer

Zhang G,Lan B,Zhang X,Lin M,Liu Y,Chen J,Guo F

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Mon May 01 00:00:00 EDT 2023

Sustained release of therapeutic gene by injectable hydrogel for hepatocellular carcinoma.

International journal of pharmaceutics: X

Xu S,Cai J,Cheng H,Wang W

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2023

Contribution of Mesenchymal Stem Cells from Obese Adipose Tissue to PD-L1 Over-Expression and Breast Cancer Progression through Pathogenic Th17 Cell Activation.

Cancers

Blangero F,Robert M,Andraud T,Dumontet C,Vidal H,Eljaafari A

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Mon May 29 00:00:00 EDT 2023

BRAF D594A mutation defines a unique biological and immuno-modulatory subgroup associated with functional CD8⁺ T cell infiltration in colorectal cancer.

Journal of translational medicine

Li W,Zhao C,Li W,Gong Y,Ma K,Lu Y,Liu X,Zhang L,Guo F

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Wed Oct 18 00:00:00 EDT 2023

Modification of PLAC8 by UFM1 affects tumorous proliferation and immune response by impacting PD-L1 levels in triple-negative breast cancer.

Journal for immunotherapy of cancer

Mao M,Chen Y,Yang J,Cheng Y,Xu L,Ji F,Zhou J,Zhang X,Li Z,Chen C,Ju S,Zhang J,Wang L

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Heterogeneity of tyrosine-based melanin anabolism regulates pulmonary and cerebral organotropic colonization microenvironment of melanoma cells.

Theranostics

Wang X,Chen Y,Lan B,Wang Y,Lin W,Jiang X,Ye J,Shang B,Feng C,Liu J,Zhai J,Xu M,Li Q,Lin L,Hu M,Zheng F,Chen L,Shao C,Wang Y,Shi Y

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Thu Apr 21 00:00:00 EDT 2022

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

5-Azacytidine-Mediated Modulation of the Immune Microenvironment in Murine Acute Myeloid Leukemia.

Cancers

Ebelt ND,Manuel ER

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Sun Dec 25 00:00:00 EST 2022

Isotope tracing reveals distinct substrate preference in murine melanoma subtypes with differing anti-tumor immunity.

Cancer & metabolism

Zhang X,Halberstam AA,Zhu W,Leitner BP,Thakral D,Bosenberg MW,Perry RJ

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Multiscale imaging of therapeutic anti-PD-L1 antibody localization using molecularly defined imaging agents.

Journal of nanobiotechnology

Hagemans IM,Wierstra PJ,Steuten K,Molkenboer-Kuenen JDM,van Dalen D,Ter Beest M,van der Schoot JMS,Ilina O,Gotthardt M,Figdor CG,Scheeren FA,Heskamp S,Verdoes M

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Wed Feb 02 00:00:00 EST 2022

Radiation-induced abscopal effect and its enhancement by programmed cell death 1 blockade in the hepatocellular carcinoma: A murine model study.

Clinical and molecular hepatology

Yoo GS,Ahn WG,Kim SY,Kang W,Choi C,Park HC

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Oncolytic herpes simplex virus HF10 (canerpaturev) promotes accumulation of CD8⁺ PD-1^- tumor-infiltrating T cells in PD-L1-enriched tumor microenvironment.

International journal of cancer

Eissa IR,Mukoyama N,Abdelmoneim M,Naoe Y,Matsumura S,Bustos-Villalobos I,Ichinose T,Miyajima N,Morimoto D,Tanaka M,Fujimoto Y,Sone M,Kodera Y,Kasuya H

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Collagenase-Expressing Salmonella Targets Major Collagens in Pancreatic Cancer Leading to Reductions in Immunosuppressive Subsets and Tumor Growth.

Cancers

Ebelt ND,Zamloot V,Zuniga E,Passi KB,Sobocinski LJ,Young CA,Blazar BR,Manuel ER

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

Ketogenic diet and ketone bodies enhance the anticancer effects of PD-1 blockade.

JCI insight

Ferrere G,Tidjani Alou M,Liu P,Goubet AG,Fidelle M,Kepp O,Durand S,Iebba V,Fluckiger A,Daillère R,Thelemaque C,Grajeda-Iglesias C,Alves Costa Silva C,Aprahamian F,Lefevre D,Zhao L,Ryffel B,Colomba E,Arnedos M,Drubay D,Rauber C,Raoult D,Asnicar F,Spector T,Segata N,Derosa L,Kroemer G,Zitvogel L

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Mon Jan 25 00:00:00 EST 2021

USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade.

Nature communications

Yang Z,Xu G,Wang B,Liu Y,Zhang L,Jing T,Tang M,Xu X,Jiao K,Xiang L,Fu Y,Tang D,Zhang X,Jin W,Zhuang G,Zhao X,Liu Y

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Wed Aug 11 00:00:00 EDT 2021

Antigen-Capturing Mesoporous Silica Nanoparticles Enhance the Radiation-Induced Abscopal Effect in Murine Hepatocellular Carcinoma Hepa1-6 Models.

Pharmaceutics

Yang K,Choi C,Cho H,Ahn WG,Kim SY,Shin SW,Kim Y,Jang T,Lee N,Park HC

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

Downregulation of PD-L1 via amide analogues of brefelamide: Alternatives to antibody-based cancer immunotherapy.

Experimental and therapeutic medicine

Zhang J,Yamada O,Kida S,Murase S,Hattori T,Oshima Y,Kikuchi H

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation.

Journal for immunotherapy of cancer

Wu B,Chiang HC,Sun X,Yuan B,Mitra P,Hu Y,Curiel TJ,Li R

46-5982 was used in Flow cytometry/Cell sorting to uncover a previously unrecognised role of adipocyte PD-L1 in mitigating obesity-related inflammation and metabolic dysfunction.

Sat Aug 01 00:00:00 EDT 2020

Genetic ablation of adipocyte PD-L1 reduces tumor growth but accentuates obesity-associated inflammation.

Journal for immunotherapy of cancer

Wu B,Chiang HC,Sun X,Yuan B,Mitra P,Hu Y,Curiel TJ,Li R

46-5982 was used in Flow cytometry/Cell sorting to uncover a previously unrecognised role of adipocyte PD-L1 in mitigating obesity-related inflammation and metabolic dysfunction.

Sat Aug 01 00:00:00 EDT 2020

Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms.

Journal for immunotherapy of cancer

Neuwelt AJ,Kimball AK,Johnson AM,Arnold BW,Bullock BL,Kaspar RE,Kleczko EK,Kwak JW,Wu MH,Heasley LE,Doebele RC,Li HY,Nemenoff RA,Clambey ET

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Nuclear imaging-guided PD-L1 blockade therapy increases effectiveness of cancer immunotherapy.

Journal for immunotherapy of cancer

Gao H,Wu Y,Shi J,Zhang X,Liu T,Hu B,Jia B,Wan Y,Liu Z,Wang F

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Optimized Protocol for Isolation of Small Extracellular Vesicles from Human and Murine Lymphoid Tissues.

International journal of molecular sciences

Bordas M,Genard G,Ohl S,Nessling M,Richter K,Roider T,Dietrich S,Maaß KK,Seiffert M

46-5982 was used in Flow cytometry/Cell sorting to demonstrate the superiority of size-exclusion chromatography, which resulted in a higher yield and purity of sEVs, and we show that their functionality alters significantly between the two isolation protocols.

Tue Aug 04 00:00:00 EDT 2020

Epigenetically modulated FOXM1 suppresses dendritic cell maturation in pancreatic cancer and colon cancer.

Molecular oncology

Zhou Z,Chen H,Xie R,Wang H,Li S,Xu Q,Xu N,Cheng Q,Qian Y,Huang R,Shao Z,Xiang M

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

PD-L1 in tumor microenvironment mediates resistance to oncolytic immunotherapy.

The Journal of clinical investigation

Zamarin D,Ricca JM,Sadekova S,Oseledchyk A,Yu Y,Blumenschein WM,Wong J,Gigoux M,Merghoub T,Wolchok JD

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Mon Apr 02 00:00:00 EDT 2018

Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

Nature communications

Sun C,Lan P,Han Q,Huang M,Zhang Z,Xu G,Song J,Wang J,Wei H,Zhang J,Sun R,Zhang C,Tian Z

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Wed Mar 28 00:00:00 EDT 2018

PDL2⁺ CD11b⁺ dermal dendritic cells capture topical antigen through hair follicles to prime LAP⁺ Tregs.

Nature communications

Tordesillas L,Lozano-Ojalvo D,Dunkin D,Mondoulet L,Agudo J,Merad M,Sampson HA,Berin MC

46-5982 was used in Flow cytometry/Cell sorting to show how topical antigen is acquired and presented by dendritic cells in the skin.

Fri Dec 07 00:00:00 EST 2018

Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes.

Scientific reports

Osum KC,Burrack AL,Martinov T,Sahli NL,Mitchell JS,Tucker CG,Pauken KE,Papas K,Appakalai B,Spanier JA,Fife BT

46-5982 was used in Flow cytometry/Cell sorting to suggest that insulin-producing Β cells respond to pancreatic inflammation and IFN-γ production by upregulating PD-L1 expression to limit self-reactive T cells.

Tue May 29 00:00:00 EDT 2018

Nuclear receptor NR2F6 inhibition potentiates responses to PD-L1/PD-1 cancer immune checkpoint blockade.

Nature communications

Klepsch V,Hermann-Kleiter N,Do-Dinh P,Jakic B,Offermann A,Efremova M,Sopper S,Rieder D,Krogsdam A,Gamerith G,Perner S,Tzankov A,Trajanoski Z,Wolf D,Baier G

46-5982 was used in Flow cytometry/Cell sorting to define NR2F6 as an intracellular immune checkpoint with potential as a therapeutic target in cancer treatment.

Wed Apr 18 00:00:00 EDT 2018

Oncogenic RAS Signaling Promotes Tumor Immunoresistance by Stabilizing PD-L1 mRNA.

Immunity

Coelho MA,de Carné Trécesson S,Rana S,Zecchin D,Moore C,Molina-Arcas M,East P,Spencer-Dene B,Nye E,Barnouin K,Snijders AP,Lai WS,Blackshear PJ,Downward J

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Tue Dec 19 00:00:00 EST 2017

Fractional Laser Releases Tumor-Associated Antigens in Poorly Immunogenic Tumor and Induces Systemic Immunity.

Scientific reports

Kawakubo M,Cunningham TJ,Demehri S,Manstein D

46-5982 was used in Flow cytometry/Cell sorting to assess the utility of ablative fractional photothermolysis for treating oncological indications.

Fri Oct 06 00:00:00 EDT 2017

Ionizing radiation sensitizes tumors to PD-L1 immune checkpoint blockade in orthotopic murine head and neck squamous cell carcinoma.

Oncoimmunology

Oweida A,Lennon S,Calame D,Korpela S,Bhatia S,Sharma J,Graham C,Binder D,Serkova N,Raben D,Heasley L,Clambey E,Nemenoff R,Karam SD

46-5982 was used in Flow cytometry/Cell sorting to investigate whether local radiation can transform the tumour immune landscape and render poorly immunogenic head and neck squamous cell carcinoma tumours sensitive to PD-L1 inhibition.

Fri Apr 08 00:00:00 EDT 2022

Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission.

Immunity

Tian Y,Kuo CF,Akbari O,Ou JH

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Tue May 17 00:00:00 EDT 2016

Ex vivo expanded hematopoietic stem cells overcome the MHC barrier in allogeneic transplantation.

Cell stem cell

Zheng J,Umikawa M,Zhang S,Huynh H,Silvany R,Chen BP,Chen L,Zhang CC

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Fri Aug 05 00:00:00 EDT 2011

Farnesyltransferase inhibitor FTI-277 reduces mortality of septic mice along with improved bacterial clearance.

The Journal of pharmacology and experimental therapeutics

Yang W,Yamada M,Tamura Y,Chang K,Mao J,Zou L,Feng Y,Kida K,Scherrer-Crosbie M,Chao W,Ichinose F,Yu YM,Fischman AJ,Tompkins RG,Yao S,Kaneki M

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 46-5982-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1.

Cell research

Sheng H,Wang Y,Jin Y,Zhang Q,Zhang Y,Wang L,Shen B,Yin S,Liu W,Cui L,Li N

Fri Aug 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1

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Gene Aliases: A530045L16Rik; B7h1; Cd274; Pdcd1l1; Pdcd1lg1; Pdl1

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UniProt ID: (Mouse) Q9EP73

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Entrez Gene ID: (Mouse) 60533

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Function(s)

protein binding immunoglobulin receptor superfamily

Process(es)

immune response signal transduction cell surface receptor signaling pathway positive regulation of cell migration T cell costimulation negative regulation of interferon-gamma production negative regulation of interleukin-10 production positive regulation of T cell proliferation negative regulation of T cell proliferation negative regulation of activated T cell proliferation toxin transport negative regulation of tumor necrosis factor superfamily cytokine production positive regulation of interleukin-10 secretion

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), PerCP-eFluor™ 710, eBioscience™

CD274 (PD-L1, B7-H1) Antibody (46-5982-82) in Flow

Product Details

46-5982-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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