Invitrogen

CD27 Monoclonal Antibody (LG.7F9), PE-Cyanine7, eBioscience™

FIGURE: 1 / 21

CD27 Antibody (25-0271-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Armenian Hamster IgG Isotype Control PE-Cyanine7 (Product # 25-4888-82) (left) or 0.25 µg of Anti-CD27 PE-Cyanine7 (right). Total viable cells were used for analysis.

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Product Details

25-0271-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 30 publications 30 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

LG.7F9

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1724035

Product Specific Information

Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46^-CD8^- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4^+ and CD8^+ effector cells. LG.7F9 cross-reacts with human and rat CD27.

Applications Reported: This LG.7F9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using CD27 monoclonal antibody (Product # 25-0271-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Cross-protective antibodies against common endemic respiratory viruses.

Nature communications

Cabán M,Rodarte JV,Bibby M,Gray MD,Taylor JJ,Pancera M,Boonyaratanakornkit J

25-0271-82 was used in Flow cytometry/Cell sorting to present the discovery, in vitro characterization, and in vivo efficacy testing of two cross-neutralizing monoclonal antibodies, one targeting both HPIV3 and HPIV1 and the other targeting both RSV and HMPV.

Mon Feb 13 00:00:00 EST 2023

Chronic CD27-CD70 costimulation promotes type 1-specific polarization of effector Tregs.

Frontiers in immunology

Bowakim-Anta N,Acolty V,Azouz A,Yagita H,Leo O,Goriely S,Oldenhove G,Moser M

25-0271-82 was used in Flow cytometry/Cell sorting to conclude that CD27 may regulate the development of Th1 immunity in peripheral tissues as well as the subsequent switch of the effector response into long-term memory.

Thu Apr 06 00:00:00 EDT 2023

The transcription factor Bach2 negatively regulates murine natural killer cell maturation and function.

eLife

Li S,Bern MD,Miao B,Fan C,Xing X,Inoue T,Piersma SJ,Wang T,Colonna M,Kurosaki T,Yokoyama WM

Published figure using CD27 monoclonal antibody (Product # 25-0271-82) in Flow Cytometry

Mon Oct 03 00:00:00 EDT 2022

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

Published figure using CD27 monoclonal antibody (Product # 25-0271-82) in Flow Cytometry

Mon Jun 07 00:00:00 EDT 2021

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

Published figure using CD27 monoclonal antibody (Product # 25-0271-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using CD27 monoclonal antibody (Product # 25-0271-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Effect of Subcutaneous Anti-CD20 Antibody-Mediated B Cell Depletion on Susceptibility to Pneumocystis Infection in Mice.

mSphere

Dai G,Noell K,Weckbecker G,Kolls JK

Published figure using CD27 monoclonal antibody (Product # 25-0271-82) in Flow Cytometry

Wed May 05 00:00:00 EDT 2021

Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection.

Cell reports. Medicine

Rouers A,Chng MHY,Lee B,Rajapakse MP,Kaur K,Toh YX,Sathiakumar D,Loy T,Thein TL,Lim VWX,Singhal A,Yeo TW,Leo YS,Vora KA,Casimiro D,Lim B,Tucker-Kellogg L,Rivino L,Newell EW,Fink K

Published figure using CD27 monoclonal antibody (Product # 25-0271-82) in Flow Cytometry

Tue May 18 00:00:00 EDT 2021

Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies.

Cell reports

Seydoux E,Wan YH,Feng J,Wall A,Aljedani S,Homad LJ,MacCamy AJ,Weidle C,Gray MD,Brumage L,Taylor JJ,Pancera M,Stamatatos L,McGuire AT

25-0271-82 was used in Flow Cytometry to characterize a panel of anti-idiotypic monoclonal antibodies (ai-mAbs) raised against inferred-germline (iGL) VRC01-class antibodies.

Tue May 04 00:00:00 EDT 2021

Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies.

Cell reports

Seydoux E,Wan YH,Feng J,Wall A,Aljedani S,Homad LJ,MacCamy AJ,Weidle C,Gray MD,Brumage L,Taylor JJ,Pancera M,Stamatatos L,McGuire AT

25-0271-82 was used in Flow Cytometry to characterize a panel of anti-idiotypic monoclonal antibodies (ai-mAbs) raised against inferred-germline (iGL) VRC01-class antibodies.

Tue May 04 00:00:00 EDT 2021

Ontogenic timing, T cell receptor signal strength, and Notch signaling direct γδ T cell functional differentiation in vivo.

Cell reports

Chen ELY,Lee CR,Thompson PK,Wiest DL,Anderson MK,Zúñiga-Pflücker JC

Published figure using CD27 monoclonal antibody (Product # 25-0271-82) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

Interleukin-17A Serves a Priming Role in Autoimmunity by Recruiting IL-1β-Producing Myeloid Cells that Promote Pathogenic T Cells.

Immunity

McGinley AM,Sutton CE,Edwards SC,Leane CM,DeCourcey J,Teijeiro A,Hamilton JA,Boon L,Djouder N,Mills KHG

25-0271 was used in Flow cytometry/Cell sorting to demonstrate that a key function of IL-17A in central nervous system (CNS) autoimmunity is to recruit IL-1β-secreting myeloid cells that prime pathogenic γδT17 and Th17 cells.

Tue Feb 18 00:00:00 EST 2020

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using CD27 monoclonal antibody (Product # 25-0271-82) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Stage-Specific Requirement for Eomes in Mature NK Cell Homeostasis and Cytotoxicity.

Cell reports

Wagner JA,Wong P,Schappe T,Berrien-Elliott MM,Cubitt C,Jaeger N,Lee M,Keppel CR,Marin ND,Foltz JA,Marsala L,Neal CC,Sullivan RP,Schneider SE,Keppel MP,Saucier N,Cooper MA,Fehniger TA

25-0271 was used in Flow cytometry/Cell sorting to develop a tamoxifen-inducible, type-1-ILC-specific (Ncr1-targeted) cre mouse and combine this with Eomes-floxed mice.

Tue Jun 02 00:00:00 EDT 2020

CD4⁺ T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium Development.

Frontiers in immunology

Cobbold SP,Adams E,Howie D,Waldmann H

25-0271 was used in Flow cytometry/Cell sorting to develop an in vitro model of T cell differentiation suited to testing how manipulation of components of the microenvironment might be therapeutically exploited.

Mon Jan 31 00:00:00 EST 2022

BALDR: a computational pipeline for paired heavy and light chain immunoglobulin reconstruction in single-cell RNA-seq data.

Genome medicine

Upadhyay AA,Kauffman RC,Wolabaugh AN,Cho A,Patel NB,Reiss SM,Havenar-Daughton C,Dawoud RA,Tharp GK,Sanz I,Pulendran B,Crotty S,Lee FE,Wrammert J,Bosinger SE

Published figure using CD27 monoclonal antibody (Product # 25-0271-82) in Flow Cytometry

Tue Mar 20 00:00:00 EDT 2018

Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function.

Nature communications

Wang F,Meng M,Mo B,Yang Y,Ji Y,Huang P,Lai W,Pan X,You T,Luo H,Guan X,Deng Y,Yuan S,Chu J,Namaka M,Hughes T,Ye L,Yu J,Li X,Deng Y

Published figure using CD27 monoclonal antibody (Product # 25-0271-82) in Flow Cytometry

Mon Nov 19 00:00:00 EST 2018

FHL2 Regulates Natural Killer Cell Development and Activation during Streptococcus pneumoniae Infection.

Frontiers in immunology

Baranek T,Morello E,Valayer A,Aimar RF,Bréa D,Henry C,Besnard AG,Dalloneau E,Guillon A,Dequin PF,Narni-Mancinelli E,Vivier E,Laurent F,Wei Y,Paget C,Si-Tahar M

25-0271 was used in Flow cytometry/Cell sorting to determine the expression and role of FHL2 in natural killer cell biology.

Wed Nov 20 00:00:00 EST 2019

Mammary tumor-derived CCL2 enhances pro-metastatic systemic inflammation through upregulation of IL1β in tumor-associated macrophages.

Oncoimmunology

Kersten K,Coffelt SB,Hoogstraat M,Verstegen NJM,Vrijland K,Ciampricotti M,Doornebal CW,Hau CS,Wellenstein MD,Salvagno C,Doshi P,Lips EH,Wessels LFA,de Visser KE

25-0271 was used in Flow cytometry/Cell sorting to demonstrate mammary tumor-derived CCL2 can facilitate the breast cancer-induced pro-metastatic systemic inflammatory gamma delta T cell-IL17-neutrophil axis.

Sat Jan 29 00:00:00 EST 2022

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD27 monoclonal antibody (Product # 25-0271-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

Published figure using CD27 monoclonal antibody (Product # 25-0271-82) in Flow Cytometry

Wed Sep 07 00:00:00 EDT 2016

Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells.

Nature immunology

Laidlaw BJ,Cui W,Amezquita RA,Gray SM,Guan T,Lu Y,Kobayashi Y,Flavell RA,Kleinstein SH,Craft J,Kaech SM

Published figure using CD27 monoclonal antibody (Product # 25-0271-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer.

Cancer immunology research

Severson JJ,Serracino HS,Mateescu V,Raeburn CD,McIntyre RC,Sams SB,Haugen BR,French JD

25-0271 was used in Flow cytometry/Cell sorting to determine that the release of PD-1 and Tim-3, and the inhibition of Tregs, may encourage tumour elimination in metastatic differentiated thyroid cancer.

Mon Jun 01 00:00:00 EDT 2015

Activated NKT cells imprint NK-cell differentiation, functionality and education.

European journal of immunology

Riese P,Trittel S,May T,Cicin-Sain L,Chambers BJ,Guzmán CA

25-0271 was used in Flow cytometry/Cell sorting to study the cellular cross-talk between natural killer T cells and natural killer cells.

Mon Jun 01 00:00:00 EDT 2015

The tyrosine phosphatase PTPN22 discriminates weak self peptides from strong agonist TCR signals.

Nature immunology

Salmond RJ,Brownlie RJ,Morrison VL,Zamoyska R

25-0271 was used in Flow cytometry/Cell sorting to study how loss-of-function PTPN22 alleles can lead to the population expansion of effector and/or memory T cells and a predisposition to human autoimmunity.

Mon Sep 01 00:00:00 EDT 2014

Type I interferon signaling enhances CD8+ T cell effector function and differentiation during murine gammaherpesvirus 68 infection.

Journal of virology

Jennings RN,Grayson JM,Barton ES

25-0271 was used in Flow cytometry/Cell sorting to assess T cell responses in IFNAR1(-/-) mice after MHV68 infection.

Mon Dec 01 00:00:00 EST 2014

Human γδ thymocytes are functionally immature and differentiate into cytotoxic type 1 effector T cells upon IL-2/IL-15 signaling.

Journal of immunology (Baltimore, Md. : 1950)

Ribot JC,Ribeiro ST,Correia DV,Sousa AE,Silva-Santos B

25-0271 was used in Flow cytometry/Cell sorting to study how ex vivo-isolated γδ thymocytes produced negligible IFN-γ and lacked cytolytic activity against leukemia cells.

Sat Mar 01 00:00:00 EST 2014

Plasmacytoid dendritic cells are scarcely represented in the human gut mucosa and are not recruited to the celiac lesion.

Mucosal immunology

Ráki M,Beitnes AC,Lundin KE,Jahnsen J,Jahnsen FL,Sollid LM

25-0271 was used in Flow cytometry/Cell sorting to suggest that IFN-α is present in the celiac mucosa and IFN-λ is important in intestinal antiviral defense.

Sun Sep 01 00:00:00 EDT 2013

Markers of nonselective and specific NK cell activation.

Journal of immunology (Baltimore, Md. : 1950)

Fogel LA,Sun MM,Geurs TL,Carayannopoulos LN,French AR

25-0271 was used in Flow cytometry/Cell sorting to demonstrate that a combination of Sca-1, CD27, and KLRG1 can distinguish NK cells based on their mechanism of activation.

Sat Jun 15 00:00:00 EDT 2013

Programmed death-1+ T cells and regulatory T cells are enriched in tumor-involved lymph nodes and associated with aggressive features in papillary thyroid cancer.

The Journal of clinical endocrinology and metabolism

French JD,Kotnis GR,Said S,Raeburn CD,McIntyre RC,Klopper JP,Haugen BR

25-0271 was used in Flow cytometry/Cell sorting to characterise the CD4(+) T cell response to metastatic PTC.

Fri Jun 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD antigen 27; CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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Gene Aliases: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

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UniProt ID: (Human) P26842, (Mouse) P41272

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Entrez Gene ID: (Human) 939, (Mouse) 21940, (Rat) 500318

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Function(s)

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process molecular_function

Process(es)

inflammatory response immune response cell surface receptor signaling pathway brain development release of cytoplasmic sequestered NF-kappaB extrinsic apoptotic signaling pathway via death domain receptors immunoglobulin mediated immune response response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade response to ethanol positive regulation of B cell differentiation positive regulation of JNK cascade negative regulation of T cell apoptotic process extrinsic apoptotic signaling pathway apoptotic process B cell proliferation positive regulation of interferon-gamma biosynthetic process positive regulation of T cell differentiation immunoglobulin secretion

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD27 Monoclonal Antibody (LG.7F9), PE-Cyanine7, eBioscience™

CD27 Antibody (25-0271-82) in Flow

Product Details

25-0271-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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