Invitrogen

CD262 (DR5) Monoclonal Antibody (MD5-1), PE, eBioscience™

CD262 (DR5) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

CD262 (DR5) Antibody (12-5883-82) in Flow

Staining of non-transfected (left) and mouse DR5 transfected (right) BHK cells with 0.125 µg of Golden Syrian Hamster IgG Isotype Control PE (Product # 12-4914-81) (open histogram) or 0.125 µg of Anti-Mouse CD262 (DR5) PE (filled histogram). Total viable cells were used for analysis.

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Product Details

12-5883-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MD5-1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465999

Product Specific Information

Description: The MD5-1 monoclonal antibody reacts with mouse DR5, also known as TRAIL-R2, Apo2, TRICK2, and KILLER. DR5 binds to TRAIL, activates NF-kappaB, and induces TRAIL-mediated apoptosis. DR5 mRNA is expressed broadly by normal tissue and human antigen is also expressed by some tumor cell lines.

Applications Reported: The MD5-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The MD5-1 antibody has been tested by flow cytometric analysis of mouse DR5-transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

DR5 (Apo2, TRAIL-R2, TRICK2, KILLER) is a recently identified death domain containing receptor for TRAIL, which mediates TRAIL induced apoptosis. DR5 is a member of the TNF-receptor superfamily, and contains an intracellular death domain. DR5 can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by DR5. Two transcript variants encoding different isoforms of DR5 and one non-coding transcript have been found. Overexpression of DR5 induces apoptosis and activates NF-kappa-B. DR5 is expressed in a number of cell types, and to particularly high levels in lymphocytes and spleen. Diseases associated with DR5 dysfunction include squamous cell carcinoma, and diffuse infiltrative lymphocytosis syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells.

Scientific reports

Knox T,Sahakian E,Banik D,Hadley M,Palmer E,Noonepalle S,Kim J,Powers J,Gracia-Hernandez M,Oliveira V,Cheng F,Chen J,Barinka C,Pinilla-Ibarz J,Lee NH,Kozikowski A,Villagra A

12-5883-82 was used in Flow Cytometry to reveal the cellular components that mediate the effect selective inhibition of HDAC6 on decreasing tumour growth in several malignancies.

Tue Apr 16 00:00:00 EDT 2019

N-glycosylation of mouse TRAIL-R restrains TRAIL-induced apoptosis.

Cell death & disease

Estornes Y,Dondelinger Y,Weber K,Bruggeman I,Peall A,MacFarlane M,Lebecque S,Vandenabeele P,Bertrand MJM

12-5883 was used in Flow cytometry/Cell sorting to demonstrate that inhibition of N-glycosylation of mTRAIL-R, and not endoplasmic reticulum stress induction, sensitizes mouse cells to TRAIL-induced apoptosis.

Tue May 01 00:00:00 EDT 2018

Engineered erythrocytes covalently linked to antigenic peptides can protect against autoimmune disease.

Proceedings of the National Academy of Sciences of the United States of America

Pishesha N,Bilate AM,Wibowo MC,Huang NJ,Li Z,Deshycka R,Bousbaine D,Li H,Patterson HC,Dougan SK,Maruyama T,Lodish HF,Ploegh HL

12588382 was used in flow cytometry to investigate the use of red blood cells expressing disease-associated autoantigenas a means of inducing antigen-specific tolerance

Tue Mar 21 00:00:00 EDT 2017

Transcriptional-mediated effects of radiation on the expression of immune susceptibility markers in melanoma.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology

Werner LR,Kler JS,Gressett MM,Riegert M,Werner LK,Heinze CM,Kern JG,Abbariki M,Erbe AK,Patel RB,Sriramaneni RN,Harari PM,Morris ZS

12-5883 was used in Flow cytometry/Cell sorting to study the transcriptional-mediated effects of radiation on the expression of immune susceptibility markers in melanoma.

Fri Sep 01 00:00:00 EDT 2017

Fasting enhances TRAIL-mediated liver natural killer cell activity via HSP70 upregulation.

PloS one

Dang VT,Tanabe K,Tanaka Y,Tokumoto N,Misumi T,Saeki Y,Fujikuni N,Ohdan H

12-5883 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms underlying the enhancement of natural killer cell function by fasting in mice.

Mon Jun 29 00:00:00 EDT 2015

Puma and Trail/Dr5 pathways control radiation-induced apoptosis in distinct populations of testicular progenitors.

PloS one

Coureuil M,Ugolin N,Tavernier M,Chevillard S,Barroca V,Fouchet P,Allemand I

12-5883 was used in Flow cytometry/Cell sorting to demonstrate that two p53-dependent pathways are involved in radiation-induced apoptosis of spermatogonia in mice.

Thu Aug 12 00:00:00 EDT 2010

Small molecule XIAP inhibitors enhance TRAIL-induced apoptosis and antitumor activity in preclinical models of pancreatic carcinoma.

Cancer research

Vogler M,Walczak H,Stadel D,Haas TL,Genze F,Jovanovic M,Bhanot U,Hasel C,Möller P,Gschwend JE,Simmet T,Debatin KM,Fulda S

12-5883 was used in Flow cytometry/Cell sorting to provide evidence that XIAP inhibitors prime pancreatic carcinoma cells for TRAIL-induced apoptosis and potentiate TRAIL antitumour activity against pancreatic carcinoma.

Sun Mar 15 00:00:00 EDT 2009

CD8 T cells utilize TRAIL to control influenza virus infection.

Journal of immunology (Baltimore, Md. : 1950)

Brincks EL,Katewa A,Kucaba TA,Griffith TS,Legge KL

12-5883 was used in Flow cytometry/Cell sorting to determine that TRAIL deficiency decreases CD8(+) T cell-mediated cytotoxicity.

Wed Oct 01 00:00:00 EDT 2008

Characterization of fibroblasts responsible for cartilage destruction in arthritis.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Treese C,Mittag A,Lange F,Tarnok A,Loesche A,Emmrich F,Lehmann J,Sack U

12-5883 was used in Flow cytometry/Cell sorting to show that LS48 is an excellent tool to gain a deeper understanding of synovial fibroblasts in rheumatoid arthritis.

Tue Apr 01 00:00:00 EDT 2008

Combination of treatment with death receptor 5-specific antibody with therapeutic HPV DNA vaccination generates enhanced therapeutic anti-tumor effects.

Vaccine

Tseng CW,Monie A,Trimble C,Alvarez RD,Huh WK,Buchsbaum DJ,Straughn JM,Wang MC,Yagita H,Hung CF,Wu TC

12-5883 was used in Flow cytometry/Cell sorting to indicate that mice with E7-expressing tumor, TC-1 treated with MD5-1 followed by CRT/E7 DNA vaccination generated potent therapeutic anti-tumor effects.

Tue Aug 12 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD262; Death receptor 5; dr-5; KILLER/DR5 TRAIL death-inducing receptor; MK; sCD262; soluble CD262; Tumor necrosis factor receptor superfamily member 10B

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Gene Aliases: DR5; KILLER; Ly98; MK; Tnfrsf10b; TRAILR2; TRICK2A; TRICK2B; TRICKB

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UniProt ID: (Mouse) Q9QZM4

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Entrez Gene ID: (Mouse) 21933

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Function(s)

protease binding transcription factor binding cysteine-type endopeptidase activator activity involved in apoptotic process TRAIL binding transmembrane signal receptor

Process(es)

apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process signal transduction extrinsic apoptotic signaling pathway via death domain receptors TRAIL-activated apoptotic signaling pathway regulation of apoptotic process extrinsic apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD262 (DR5) Monoclonal Antibody (MD5-1), PE, eBioscience™

CD262 (DR5) Antibody (12-5883-82) in Flow

Product Details

12-5883-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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