Invitrogen

CD206 (MMR) Monoclonal Antibody (MR6F3), APC-eFluor™ 780, eBioscience™

CD206 (MMR) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 53

CD206 (MMR) Antibody (47-2061-82) in Flow

BALB/c mouse resident peritoneal macrophages were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with F4/80 Monoclonal Antibody, FITC (Product # 11-4801-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, APC-eFluor 780 (Product # 47-4031-82) (left) or 0.25 µg of CD206 Monoclonal Antibody, APC-eFluor 780 (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).

CD206 (MMR) Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

47-2061-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 35 publications 35 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MR6F3

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802285

Product Specific Information

Description: This MR6F3 antibody recognizes mouse CD206 also known as Macrophage Mannose Receptor (MMR) or Mannose Receptor C, Type 1 (MRC1). CD206 is a 175-kDa, type 1 integral membrane glycoprotein receptor that is present in macrophages, some dendritic cells, as well as liver and lymphoid endothelial cells. CD206 belongs to the C-type lectin family. Extracellular regions of CD206 include an N-terminal cysteine-rich (CR) domain that binds sulfated glycoproteins, a fibronectin II (FNII) domain that binds collagens, and eight carbohydrate recognition domains (CRDs) that bind sugars like mannose and fucose with high affinity. CD206 mediates phagocytic and endocytic uptake of fungal, bacterial, protozoan and viral antigens, and plays an important role in immune defense and immune regulation. A soluble form of CD206 is generated by cleavage of the full-length protein, and it can be detected in in vitro macrophage cell culture supernatants and in mouse serum. CD206 is considered to be one of the markers of M2 macrophages. Factors inducing its expression include: IL-4, IL-13, M-CSF, IL-6, IL-10, and glucocorticoids, while TNF-alpha, IFN-gamma, TGF-beta, and LPS have been reported to down-regulate expression of CD206.

It has been demonstrated that only a small fraction of CD206 is present at the cell surface, therefore, intracellular staining is recommended.

Applications Reported: This MR6F3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MR6F3 antibody has been tested by intracellular staining followed by flow cytometric analysis of resident peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at www.thermofisher.com/flowprotocols. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Target Information

CD206 (MSR, Mannose receptor, MRC1) is a 175 kDa transmembrane protein belonging to the group of pattern recognition receptors. CD206 is predominantly expressed in tissue macrophages, dendritic cells, a subpopulation of endothelial cells and sperm cells. CD206 is thought to play a role in the innate and adaptive immune response. CD206 is also expressed on microglia and mato cells of the brain but not astrocytes or neurons. CD206 also mediate the recognition and uptake of a variety of macromolecules, including modified lipoproteins, advanced glycation end (AGEs) products and amyloid b-protein (Abeta). While the normal role of CD206 is associated with cell adhesion and host defense mechanisms, it also has been implicated in the development of atherosclerosis and Amyloid beta deposition in Alzheimer's disease (AD). CD206’s gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. CD206 has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. Other diseases associated with CD206 dysfunction include leprosy and Gaucher’s Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

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TREM2 expression promotes liver and peritoneal M2 macrophage polarization in mice infected with Schistosoma japonicum.

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Tue Aug 01 00:00:00 EDT 2023

A pref-1-controlled non-inflammatory mechanism of insulin resistance.

iScience

Huang Y,Cui D,Chen L,Tong H,Wu H,Muller GK,Qi Y,Wang S,Xu J,Gao X,Fifield KE,Wang L,Xia Z,Vanderluit JL,Liu S,Leng L,Sun G,McGuire J,Young LH,Bucala R,Qi D

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Spontaneous immunomodulation and regulation of angiogenesis and osteogenesis by Sr/Cu-borosilicate glass (BSG) bone cement to repair critical bone defects.

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Li S,Zhang L,Liu C,Kim J,Su K,Chen T,Zhao L,Lu X,Zhang H,Cui Y,Cui X,Yuan F,Pan H

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Mon May 01 00:00:00 EDT 2023

Hydrochlorothiazide-induced glucose metabolism disorder is mediated by the gut microbiota via LPS-TLR4-related macrophage polarization.

iScience

Luo JQ,Ren H,Chen MY,Zhao Q,Yang N,Liu Q,Gao YC,Zhou HH,Huang WH,Zhang W

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Fri Jul 21 00:00:00 EDT 2023

Dipeptidyl peptidase 4 inhibitor reduces tumor-associated macrophages and enhances anti-PD-L1-mediated tumor suppression in non-small cell lung cancer.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico

Zuo B,Li T,Liu X,Wang S,Cheng J,Liu X,Cui W,Shi H,Ling C

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Wed Nov 01 00:00:00 EDT 2023

MIR222HG attenuates macrophage M2 polarization and allergic inflammation in allergic rhinitis by targeting the miR146a-5p/TRAF6/NF-κB axis.

Frontiers in immunology

Wen S,Li F,Tang Y,Dong L,He Y,Deng Y,Tao Z

Published figure using CD206 (MMR) monoclonal antibody (Product # 47-2061-82) in Flow Cytometry

Fri May 26 00:00:00 EDT 2023

Traumatic brain injury stimulates sympathetic tone-mediated bone marrow myelopoiesis to favor fracture healing.

Signal transduction and targeted therapy

Liu W,Chen W,Xie M,Chen C,Shao Z,Zhang Y,Zhao H,Song Q,Hu H,Xing X,Cai X,Deng X,Li X,Wang P,Liu G,Xiong L,Lv X,Zhang Y

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Wed Jul 05 00:00:00 EDT 2023

Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis.

Veterinary research

Feng L,Luo G,Li Y,Zhang C,Liu Y,Liu Y,Chen H,He D,Zhu Y,Gan L

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Tue Mar 14 00:00:00 EDT 2023

Alpha-ketoglutarate promotes alveolar bone regeneration by modulating M2 macrophage polarization.

Bone reports

Li Y,Liu L,Li Y,Song W,Shao B,Li H,Lin W,Li Q,Shuai X,Bai M,Zhao B,Guo Y,Yuan Q,Wang Y

47-2061-82 was used in Flow cytometry/Cell sorting to investigate the effect of Alpha-ketoglutarate (αKG) on alveolar socket healing and reveal the underlying mechanism in the view of macrophage polarization.

Thu Jun 01 00:00:00 EDT 2023

BMP-2 functional polypeptides relieve osteolysis via bi-regulating bone formation and resorption coupled with macrophage polarization.

NPJ Regenerative medicine

Wang J,Xue Y,Wang Y,Liu C,Hu S,Zhao H,Gu Q,Yang H,Huang L,Zhou X,Shi Q

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Thu Feb 09 00:00:00 EST 2023

Oxygen-carrying nanoplatform to reprogram tumor immunosuppressive microenvironment and enhance photothermal-immunotherapy.

Materials today. Bio

Huang J,Leng X,Jiang T,Xu L,Zheng J,Fang M,Wang J,Wang Z,Zhang L

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Sat Apr 01 00:00:00 EDT 2023

Interferon-γ primes macrophages for pathogen ligand-induced killing via a caspase-8 and mitochondrial cell death pathway.

Immunity

Simpson DS,Pang J,Weir A,Kong IY,Fritsch M,Rashidi M,Cooney JP,Davidson KC,Speir M,Djajawi TM,Hughes S,Mackiewicz L,Dayton M,Anderton H,Doerflinger M,Deng Y,Huang AS,Conos SA,Tye H,Chow SH,Rahman A,Norton RS,Naderer T,Nicholson SE,Burgio G,Man SM,Groom JR,Herold MJ,Hawkins ED,Lawlor KE,Strasser A,Silke J,Pellegrini M,Kashkar H,Feltham R,Vince JE

47-2061-82 was used in Flow Cytometry to reveal that iNOS selectively licenses programmed cell death, which may explain how nitric oxide impacts disease severity in SARS-CoV-2 infection and other iNOS-associated inflammatory conditions.

Tue Mar 08 00:00:00 EST 2022

L-norvaline affects the proliferation of breast cancer cells based on the microbiome and metabolome analysis.

Journal of applied microbiology

Zhu Q,Zai H,Zhang K,Zhang X,Luo N,Li X,Hu Y,Wu Y

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Mon Aug 01 00:00:00 EDT 2022

G9a promotes inflammation in Streptococcus pneumoniae induced pneumonia mice by stimulating M1 macrophage polarization and H3K9me2 methylation in FOXP1 promoter region.

Annals of translational medicine

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Sun May 01 00:00:00 EDT 2022

Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite Stability.

Cancer immunology research

Wu Q,Huang Q,Jiang Y,Sun F,Liang B,Wang J,Hu X,Sun M,Ma Z,Shi Y,Liang Y,Tan Y,Zeng D,Yao F,Xu X,Yao Z,Li S,Rong X,Huang N,Sun L,Liao W,Shi M

Published figure using CD206 (MMR) monoclonal antibody (Product # 47-2061-82) in Flow Cytometry

Tue Feb 01 00:00:00 EST 2022

Mineralocorticoid receptor antagonists and glucocorticoids differentially affect skeletal muscle inflammation and pathology in muscular dystrophy.

JCI insight

Howard ZM,Gomatam CK,Rabolli CP,Lowe J,Piepho AB,Bansal SS,Accornero F,Rafael-Fortney JA

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Mon Oct 10 00:00:00 EDT 2022

Anti-inflammatory and immunomodulatory effects of the extracellular vesicles derived from human umbilical cord mesenchymal stem cells on osteoarthritis via M2 macrophages.

Journal of nanobiotechnology

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TIM3 Expression in Anaplastic-Thyroid-Cancer-Infiltrating Macrophages: An Emerging Immunotherapeutic Target.

Biology

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EGFR tyrosine kinase activity and Rab GTPases coordinate EGFR trafficking to regulate macrophage activation in sepsis.

Cell death & disease

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Activation of glucagon-like peptide-1 receptor in microglia attenuates neuroinflammation-induced glial scarring via rescuing Arf and Rho GAP adapter protein 3 expressions after nerve injury.

International journal of biological sciences

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Fri Apr 15 00:00:00 EDT 2022

Exosomes from human umbilical cord mesenchymal stem cells attenuate the inflammation of severe steroid-resistant asthma by reshaping macrophage polarization.

Stem cell research & therapy

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Hyperoside Suppresses Renal Inflammation by Regulating Macrophage Polarization in Mice With Type 2 Diabetes Mellitus.

Frontiers in immunology

Liu J,Zhang Y,Sheng H,Liang C,Liu H,Moran Guerrero JA,Lu Z,Mao W,Dai Z,Liu X,Zhang L

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Polarization of Type 1 Macrophages Is Associated with the Severity of Viral Encephalitis Caused by Japanese Encephalitis Virus and Dengue Virus.

Cells

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Azithromycin alleviates systemic lupus erythematosus via the promotion of M2 polarisation in lupus mice.

Cell death discovery

Wang J,Chen Q,Zhang Z,Wang S,Wang Y,Xiang M,Liang J,Xu J

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Fri Apr 16 00:00:00 EDT 2021

Resolving macrophage polarization through distinct Ca²⁺ entry channel that maintains intracellular signaling and mitochondrial bioenergetics.

iScience

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Published figure using CD206 (MMR) monoclonal antibody (Product # 47-2061-82) in Flow Cytometry

Fri Nov 19 00:00:00 EST 2021

Thymopentin treatment of murine premature ovarian failure via attenuation of immune cell activity and promotion of the BMP4/Smad9 signalling pathway.

International journal of medical sciences

Zhu X,Liu J,Pan H,Geng Z,Huang W,Liu T,Zhang B

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Electronic Cigarette Exposure Enhances Lung Inflammatory and Fibrotic Responses in COPD Mice.

Frontiers in pharmacology

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Stem cell research & therapy

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Published figure using CD206 (MMR) monoclonal antibody (Product # 47-2061-82) in Flow Cytometry

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PD-L1 Improves Motor Function and Alleviates Neuropathic Pain in Male Mice After Spinal Cord Injury by Inhibiting MAPK Pathway.

Frontiers in immunology

Kong F,Sun K,Zhu J,Li F,Lin F,Sun X,Luo X,Ren C,Lu L,Zhao S,Sun J,Wang Y,Shi J

Published figure using CD206 (MMR) monoclonal antibody (Product # 47-2061-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

STING Activated Tumor-Intrinsic Type I Interferon Signaling Promotes CXCR3 Dependent Antitumor Immunity in Pancreatic Cancer.

Cellular and molecular gastroenterology and hepatology

Vonderhaar EP,Barnekow NS,McAllister D,McOlash L,Eid MA,Riese MJ,Tarakanova VL,Johnson BD,Dwinell MB

Published figure using CD206 (MMR) monoclonal antibody (Product # 47-2061-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

Macrophage-derived EDA-A2 inhibits intestinal stem cells by targeting miR-494/EDA2R/β-catenin signaling in mice.

Communications biology

Song L,Chang R,Sun X,Lu L,Gao H,Lu H,Lin R,Xu X,Liu Z,Zhan L

Published figure using CD206 (MMR) monoclonal antibody (Product # 47-2061-82) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2021

The FBW7-MCL-1 axis is key in M1 and M2 macrophage-related colon cancer cell progression: validating the immunotherapeutic value of targeting PI3Kγ.

Experimental & molecular medicine

Lee YS,Song SJ,Hong HK,Oh BY,Lee WY,Cho YB

Published figure using CD206 (MMR) monoclonal antibody (Product # 47-2061-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

Mesenchymal stem cell-secreted extracellular vesicles carrying TGF-β1 up-regulate miR-132 and promote mouse M2 macrophage polarization.

Journal of cellular and molecular medicine

Wang Y,Han B,Wang Y,Wang C,Zhang H,Xue J,Wang X,Niu T,Niu Z,Chen Y

Published figure using CD206 (MMR) monoclonal antibody (Product # 47-2061-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Meningeal lymphatics clear erythrocytes that arise from subarachnoid hemorrhage.

Nature communications

Chen J,Wang L,Xu H,Xing L,Zhuang Z,Zheng Y,Li X,Wang C,Chen S,Guo Z,Liang Q,Wang Y

Published figure using CD206 (MMR) monoclonal antibody (Product # 47-2061-82) in Flow Cytometry

Mon Jun 22 00:00:00 EDT 2020

Macrophage MSR1 promotes the formation of foamy macrophage and neuronal apoptosis after spinal cord injury.

Journal of neuroinflammation

Kong FQ,Zhao SJ,Sun P,Liu H,Jie J,Xu T,Xu AD,Yang YQ,Zhu Y,Chen J,Zhou Z,Qian DF,Gu CJ,Chen Q,Yin GY,Zhang HW,Fan J

Published figure using CD206 (MMR) monoclonal antibody (Product # 47-2061-82) in Flow Cytometry

Mon Feb 17 00:00:00 EST 2020

Bioinformatics

Protein Aliases: CD206; Macrophage mannose receptor 1; MMR

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Gene Aliases: AW259686; CD206; MR; Mrc1

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UniProt ID: (Mouse) Q61830

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Entrez Gene ID: (Mouse) 17533

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Function(s)

receptor activity transmembrane signaling receptor activity mannose binding carbohydrate binding

Process(es)

endocytosis receptor-mediated endocytosis signal transduction cellular response to lipopolysaccharide cellular response to interferon-gamma cellular response to interleukin-4

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD206 (MMR) Monoclonal Antibody (MR6F3), APC-eFluor™ 780, eBioscience™

CD206 (MMR) Antibody (47-2061-82) in Flow

Product Details

47-2061-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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