Invitrogen

CD206 Polyclonal Antibody

CD206 Antibody in Immunocytochemistry (ICC/IF)

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CD206 Antibody (PA5-101657) in ICC/IF

Immunofluorescent analysis of CD206 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and CD206 polyclonal antibody (Product # PA5-101657) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

CD206 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD206 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-101657

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Fungi, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human MRC1(Accession P22897), corresponding to amino acid residues P642-N692.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2851091

Product Specific Information

Antibody detects endogenous levels of total MRC1.

Target Information

CD206 (MSR, Mannose receptor, MRC1) is a 175 kDa transmembrane protein belonging to the group of pattern recognition receptors. CD206 is predominantly expressed in tissue macrophages, dendritic cells, a subpopulation of endothelial cells and sperm cells. CD206 is thought to play a role in the innate and adaptive immune response. CD206 is also expressed on microglia and mato cells of the brain but not astrocytes or neurons. CD206 also mediate the recognition and uptake of a variety of macromolecules, including modified lipoproteins, advanced glycation end (AGEs) products and amyloid b-protein (Abeta). While the normal role of CD206 is associated with cell adhesion and host defense mechanisms, it also has been implicated in the development of atherosclerosis and Amyloid beta deposition in Alzheimer's disease (AD). CD206’s gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. CD206 has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. Other diseases associated with CD206 dysfunction include leprosy and Gaucher’s Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Magnesium implant degradation provides immunomodulatory and proangiogenic effects and attenuates peri-implant fibrosis in soft tissues.

Bioactive materials

Ben Amara H,Martinez DC,Shah FA,Loo AJ,Emanuelsson L,Norlindh B,Willumeit-Römer R,Plocinski T,Swieszkowski W,Palmquist A,Omar O,Thomsen P

PA5-101657 was used in Immunohistochemistry to determine the temporo-spatial cell distribution and behavior in relation to sequential changes of pure Mg implant surface properties and Mg2+ release.

Tue Aug 01 00:00:00 EDT 2023

Redox Modulatory Cu(II)-Baicalein Microflowers Prepared in One Step Effectively Promote Therapeutic Angiogenesis in Diabetic Mice.

Advanced healthcare materials

Liu K,Liu L,Guo H,Xu R,Liang X,Chen Y,Li H,Fu X,Wang X,Chen H,Li Y,Yang J

PA5-32321 was used in Immunohistochemistry-immunofluorescence to conclude that, through moderate and dynamic precise homeostasis regulation of cells.

Wed Feb 01 00:00:00 EST 2023

Decreased VEGFA alleviates the symptoms of LPS-induced sepsis in a mouse model by inhibiting glycolysis and thereby regulating the polarization of macrophages.

European journal of histochemistry : EJH

Lin J,Jiang L,Guo K,Feng N

PA5-101657 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate that decreased VEGFA inhibits glycolysis and thus inhibits LPS-induced M1 polarization of macrophages, ultimately relieving sepsis.

Mon Jan 02 00:00:00 EST 2023

Real-time visualization of phagosomal pH manipulation by Cryptococcus neoformans in an immune signal-dependent way.

Frontiers in cellular and infection microbiology

Santiago-Burgos EJ,Stuckey PV,Santiago-Tirado FH

PA5-101657 was used in Immunocytochemistry-immunofluorescence to investigate the mechanisms in Cryptococcus neoformans that alters intracellular pH during infection of host cell.

Tue Oct 11 00:00:00 EDT 2022

Real-time visualization of phagosomal pH manipulation by Cryptococcus neoformans in an immune signal-dependent way.

Frontiers in cellular and infection microbiology

Santiago-Burgos EJ,Stuckey PV,Santiago-Tirado FH

PA5-101657 was used in Immunocytochemistry-immunofluorescence to investigate the mechanisms in Cryptococcus neoformans that alters intracellular pH during infection of host cell.

Tue Oct 11 00:00:00 EDT 2022

Description of Osmolyte Pathways in Maturing Mdx Mice Reveals Altered Levels of Taurine and Sodium/Myo-Inositol Co-Transporters.

International journal of molecular sciences

Merckx C,Cosemans G,Zschüntzsch J,Raedt R,Schmidt J,De Paepe B,De Bleecker JL

PA5-101657 was used in Immunohistochemistry to highlight the potential of osmolyte pathway members as a research interest and future therapeutic target in dystrophinopathy.

Thu Mar 17 00:00:00 EDT 2022

Deficiency of ITGAM Attenuates Experimental Abdominal Aortic Aneurysm in Mice.

Journal of the American Heart Association

Zhou M,Wang X,Shi Y,Ding Y,Li X,Xie T,Shi Z,Fu W

PA5-101657 was used in Immunohistochemistry (Paraffin) to conclude that CD11b might promote the development and progression of abdominal aortic aneurysm by mediating the endothelial cells adhesion and transendothelial migration of circulating monocytes/macrophages.

Tue Apr 06 00:00:00 EDT 2021

Deficiency of ITGAM Attenuates Experimental Abdominal Aortic Aneurysm in Mice.

Journal of the American Heart Association

Zhou M,Wang X,Shi Y,Ding Y,Li X,Xie T,Shi Z,Fu W

Tue Apr 06 00:00:00 EDT 2021

Deficiency of ITGAM Attenuates Experimental Abdominal Aortic Aneurysm in Mice.

Journal of the American Heart Association

Zhou M,Wang X,Shi Y,Ding Y,Li X,Xie T,Shi Z,Fu W

Tue Apr 06 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: C-type lectin domain family 13 member D; C-type lectin domain family 13 member D-like; CD206; hMR; Human mannose receptor; Macrophage mannose receptor 1; Macrophage mannose receptor 1-like protein 1; mannose receptor, C type 1-like 1; MMR

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Gene Aliases: AW259686; bA541I19.1; CD206; CLEC13D; CLEC13DL; hMR; MMR; MR; MRC1; MRC1L1

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UniProt ID: (Human) P22897, (Mouse) Q61830

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Entrez Gene ID: (Human) 4360, (Mouse) 17533, (Rat) 291327

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Function(s)

virus receptor activity receptor activity transmembrane signaling receptor activity protein binding mannose binding carbohydrate binding

Process(es)

endocytosis receptor-mediated endocytosis signal transduction cellular response to lipopolysaccharide cellular response to interferon-gamma cellular response to interleukin-4 viral entry into host cell

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD206 Polyclonal Antibody

CD206 Antibody (PA5-101657) in ICC/IF

Product Details

PA5-101657

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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