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Invitrogen
CD170 (Siglec F) Monoclonal Antibody (1RNM44N), eBioscience™
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CD170 (Siglec F) Antibody (14-1702-82) in IHC (F)
Product Details
14-1702-82
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Product Specific Information
Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F. Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types. The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding. Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells. Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells.
This 1RNM44N antibody will recognize a formaldehyde-fixed epitope.
Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.
Applications Tested: This 1RNM44N antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 20 µg/mL. This 1RNM44N antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells and can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD170; sialic acid binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin F; sialic acid-binding lectin Siglec-F; Siglec; Siglec-5; Siglec-F
Gene Aliases: mSiglec-F; Siglec5; Siglecf
UniProt ID: (Mouse) Q920G3
Entrez Gene ID: (Mouse) 233186
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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