Invitrogen

CD170 (Siglec F) Monoclonal Antibody (1RNM44N), PE, eBioscience™

CD170 (Siglec F) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 11

CD170 (Siglec F) Antibody (12-1702-82) in Flow

Staining of mouse thioglycolate-elicited peritoneal exudate cells with Anti-Mouse F4/80 Antigen APC (Product # 17-4801-82) and 0.03 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.03 µg of Anti-Mouse CD170 (Siglec F) PE (right). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

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Product Details

12-1702-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.06 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1RNM44N

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2637129

Product Specific Information

Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F. Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types. The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding. Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells. Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells.

This 1RNM44N antibody will recognize a formaldehyde-fixed epitope.

Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Eosinophils protect against pulmonary hypertension through 14-HDHA and 17-HDHA.

The European respiratory journal

Shu T,Zhang J,Zhou Y,Chen Z,Li J,Tang Q,Lei W,Xing Y,Wang J,Wang C

12-1702-82 was used in Flow cytometry/Cell sorting to show that in PH development without external inflammatory stimulus, peripheral blood exhibits a low EOS level, in which EOS play a protective role through suppressing perivascular inflammation and maintaining PASMC homeostasis via 14/17-HDHA.

Wed Mar 01 00:00:00 EST 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD170 (Siglec F) monoclonal antibody (Product # 12-1702-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD170 (Siglec F) monoclonal antibody (Product # 12-1702-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

12-1702-82 was used in Flow cytometry/Cell sorting to demonstrate for the first time that CalD is a novel anti-inflammatory natural compound for allergic asthma that modulates AM function.

Sat Apr 01 00:00:00 EDT 2023

Group 2 innate lymphoid cells protect mouse heart from myocardial infarction injury via interleukin 5, eosinophils, and dendritic cells.

Cardiovascular research

Liu T,Meng Z,Liu J,Li J,Zhang Y,Deng Z,Luo S,Wang M,Huang Q,Zhang S,Fendt P,Devouassoux J,Li D,McKenzie ANJ,Nahrendorf M,Libby P,Guo J,Shi GP

12-1702-82 was used in Flow cytometry/Cell sorting to reveal an indirect cardiac reparative role of ILC2 in post-MI hearts via the IL5, EOS, and DC mechanism.

Tue May 02 00:00:00 EDT 2023

Cells of the human intestinal tract mapped across space and time.

Nature

Elmentaite R,Kumasaka N,Roberts K,Fleming A,Dann E,King HW,Kleshchevnikov V,Dabrowska M,Pritchard S,Bolt L,Vieira SF,Mamanova L,Huang N,Perrone F,Goh Kai'En I,Lisgo SN,Katan M,Leonard S,Oliver TRW,Hook CE,Nayak K,Campos LS,Domínguez Conde C,Stephenson E,Engelbert J,Botting RA,Polanski K,van Dongen S,Patel M,Morgan MD,Marioni JC,Bayraktar OA,Meyer KB,He X,Barker RA,Uhlig HH,Mahbubani KT,Saeb-Parsy K,Zilbauer M,Clatworthy MR,Haniffa M,James KR,Teichmann SA

Published figure using CD170 (Siglec F) monoclonal antibody (Product # 12-1702-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Arf6 exacerbates allergic asthma through cell-to-cell transmission of ASC inflammasomes.

JCI insight

Lee S,Ishitsuka A,Kuroki T,Lin YH,Shibuya A,Hongu T,Funakoshi Y,Kanaho Y,Nagata K,Kawaguchi A

Published figure using CD170 (Siglec F) monoclonal antibody (Product # 12-1702-82) in Flow Cytometry

Mon Aug 23 00:00:00 EDT 2021

Nociceptor neurons promote IgE class switch in B cells.

JCI insight

Mathur S,Wang JC,Seehus CR,Poirier F,Crosson T,Hsieh YC,Doyle B,Lee S,Woolf CJ,Foster SL,Talbot S

12-1702-82 was used in Flow cytometry/Cell sorting to suggest that nociceptors, in addition to their contributions to innate immunity, play a key role in modulating the adaptive immune response, particularly B cell antibody class switching to IgE.

Wed Dec 22 00:00:00 EST 2021

Muc5ac Expression Protects the Colonic Barrier in Experimental Colitis.

Inflammatory bowel diseases

Olli KE,Rapp C,O'Connell L,Collins CB,McNamee EN,Jensen O,Jedlicka P,Allison KC,Goldberg MS,Gerich ME,Frank DN,Ir D,Robertson CE,Evans CM,Aherne CM

12-1702 was used in Flow cytometry/Cell sorting to test the expression and functional role of MUC5AC/Muc5ac in UC biopsies and murine colitis.

Thu Aug 20 00:00:00 EDT 2020

Evaluation of K18-hACE2 Mice as a Model of SARS-CoV-2 Infection.

The American journal of tropical medicine and hygiene

Moreau GB,Burgess SL,Sturek JM,Donlan AN,Petri WA,Mann BJ

Published figure using CD170 (Siglec F) monoclonal antibody (Product # 12-1702-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Eosinophils improve cardiac function after myocardial infarction.

Nature communications

Liu J,Yang C,Liu T,Deng Z,Fang W,Zhang X,Li J,Huang Q,Liu C,Wang Y,Yang D,Sukhova GK,Lindholt JS,Diederichsen A,Rasmussen LM,Li D,Newton G,Luscinskas FW,Liu L,Libby P,Wang J,Guo J,Shi GP

Published figure using CD170 (Siglec F) monoclonal antibody (Product # 12-1702-82) in Flow Cytometry

Wed Dec 16 00:00:00 EST 2020

The cis-Regulatory Atlas of the Mouse Immune System.

Cell

Yoshida H,Lareau CA,Ramirez RN,Rose SA,Maier B,Wroblewska A,Desland F,Chudnovskiy A,Mortha A,Dominguez C,Tellier J,Kim E,Dwyer D,Shinton S,Nabekura T,Qi Y,Yu B,Robinette M,Kim KW,Wagers A,Rhoads A,Nutt SL,Brown BD,Mostafavi S,Buenrostro JD,Benoist C

12-1702-82 was used in Flow Cytometry to generate matched epigenome and transcriptome measurements in 86 primary cell types that span the mouse immune system and its differentiation cascade.

Thu Feb 07 00:00:00 EST 2019

11 Beta-hydroxysteroid dehydrogenase type 1 regulates synovitis, joint destruction, and systemic bone loss in chronic polyarthritis.

Journal of autoimmunity

Hardy RS,Fenton C,Croft AP,Naylor AJ,Begum R,Desanti G,Buckley CD,Lavery G,Cooper MS,Raza K

Published figure using CD170 (Siglec F) monoclonal antibody (Product # 12-1702-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2018

Non-redundant Functions of IL-6 Produced by Macrophages and Dendritic Cells in Allergic Airway Inflammation.

Frontiers in immunology

Gubernatorova EO,Gorshkova EA,Namakanova OA,Zvartsev RV,Hidalgo J,Drutskaya MS,Tumanov AV,Nedospasov SA

12-1702 was used in Flow cytometry/Cell sorting to indicate that IL-6 plays a pathogenic role in the HDM-induced asthma model and that lung macrophages and dendritic cells are the predominant sources of pathogenic IL-6 but contribute differently to the disease.

Wed Oct 02 00:00:00 EDT 2019

Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells.

Scientific reports

Wang H,Lin J,Zeng L,Ouyang C,Ran P,Yang P,Liu Z

Published figure using CD170 (Siglec F) monoclonal antibody (Product # 12-1702-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Expression of the inhibitory Ly49E receptor is not critically involved in the immune response against cutaneous, pulmonary or liver tumours.

Scientific reports

Filtjens J,Keirsse J,Van Ammel E,Taveirne S,Van Acker A,Kerre T,Taghon T,Vandekerckhove B,Plum J,Van Ginderachter JA,Leclercq G

12-1702 was used in Flow cytometry/Cell sorting to study the involvement of the inhibitory Ly49E NK cell receptor and its activating molecule uPA in skin, lung, and liver tumorigenesis.

Fri Jul 29 00:00:00 EDT 2016

Lung type 2 innate lymphoid cells express cysteinyl leukotriene receptor 1, which regulates TH2 cytokine production.

The Journal of allergy and clinical immunology

Doherty TA,Khorram N,Lund S,Mehta AK,Croft M,Broide DH

12-1702 was used in Flow cytometry/Cell sorting to determine if leukotrienes contribute to asthma pathogenesis by activating type 2 innate lymphoid cells and inducing interleukin production.

Mon Jul 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: CD170; sialic acid binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin 5; Sialic acid-binding Ig-like lectin F; sialic acid-binding lectin Siglec-F; Siglec; Siglec-5; Siglec-F

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Gene Aliases: mSiglec-F; Siglec5; Siglecf

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UniProt ID: (Mouse) Q920G3

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Entrez Gene ID: (Mouse) 233186

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Function(s)

carbohydrate binding monosaccharide binding immunoglobulin superfamily cell adhesion molecule

Process(es)

cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD170 (Siglec F) Monoclonal Antibody (1RNM44N), PE, eBioscience™

CD170 (Siglec F) Antibody (12-1702-82) in Flow

Product Details

12-1702-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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