Invitrogen

CD163 Monoclonal Antibody (TNKUPJ), PE, eBioscience™

FIGURE: 1 / 12

CD163 Antibody (12-1631-82) in Flow

BALB/c mouse splenocytes were stained with F4/80 Monoclonal Antibody, eFluor 450 (Product # 48-48-4801) and 0.125 µg of Rat IgG2a kappa Isotype Control, PE (Product # 12-4321) (left) or 0.125 µg of CD163 Monoclonal Antibody, PE (Product # 12-1631) (right). Total cells were used for analysis.

CD163 Antibody in Immunohistochemistry (IHC)

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Product Details

12-1631-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TNKUPJ

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2716924

Product Specific Information

Description: This TNKUPJ monoclonal antibody recognizes mouse CD163. CD163 is a 130kDa surface receptor expressed by certain subsets of tissue macrophages, including splenic red pulp macrophages, Kupffer cells, intestinal lamina propria macrophages and a small fraction of peritoneal macrophages. In contrast to human blood monocytes, mouse monocytes do not express CD163. Also, unlike human CD163, mouse CD163 is not as readily induced by M2 polarizing cytokines, and it is not a good marker of M2 macrophages. No common cell lines of monocytic or macrophage origin express mouse CD163. In humans, CD163 has been shown to be proteolytically cleaved and shed from the cell surface, and it acts as a soluble anti-inflammatory factor.

This TNKUPJ antibody will detect CD163 on fixed and permeabilized cells allowing for staining of the intracellular pool of this receptor. Although CD163 is relatively stable to collagenase digestion, aggressive tissue dissociation protocols might potentially decrease the amount of surface CD163. In these cases intracellular detection is recommended.

Applications Reported: This TNKUPJ antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TNKUPJ antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Shp2 Deficiency in Kupffer Cells and Hepatocytes Aggravates Hepatocarcinogenesis by Recruiting Non-Kupffer Macrophages.

Cellular and molecular gastroenterology and hepatology

Du L,Ji Y,Xin B,Zhang J,Lu LC,Glass CK,Feng GS

Published figure using CD163 monoclonal antibody (Product # 12-1631-82) in Flow Cytometry

Mon May 22 00:00:00 EDT 2023

Pericyte stem cells induce Ly6G⁺ cell accumulation and immunotherapy resistance in pancreatic cancer.

EMBO reports

Wu Z,Thierry K,Bachy S,Zhang X,Gamradt P,Hernandez-Vargas H,Mikaelian I,Tonon L,Pommier R,Zhao Y,Bertolino P,Hennino A

Published figure using CD163 monoclonal antibody (Product # 12-1631-82) in Flow Cytometry

Wed Apr 05 00:00:00 EDT 2023

Pericyte stem cells induce Ly6G⁺ cell accumulation and immunotherapy resistance in pancreatic cancer.

EMBO reports

Wu Z,Thierry K,Bachy S,Zhang X,Gamradt P,Hernandez-Vargas H,Mikaelian I,Tonon L,Pommier R,Zhao Y,Bertolino P,Hennino A

Published figure using CD163 monoclonal antibody (Product # 12-1631-82) in Flow Cytometry

Wed Apr 05 00:00:00 EDT 2023

DIAPH1 mediates progression of atherosclerosis and regulates hepatic lipid metabolism in mice.

Communications biology

Senatus L,Egaña-Gorroño L,López-Díez R,Bergaya S,Aranda JF,Amengual J,Arivazhagan L,Manigrasso MB,Yepuri G,Nimma R,Mangar KN,Bernadin R,Zhou B,Gugger PF,Li H,Friedman RA,Theise ND,Shekhtman A,Fisher EA,Ramasamy R,Schmidt AM

12-1631-82 was used in Flow cytometry/Cell sorting to unveil new regulators of atherosclerosis and lipid metabolism through DIAPH1.

Fri Mar 17 00:00:00 EDT 2023

Restoring Prohealing/Remodeling-Associated M2a/c Macrophages Using ON101 Accelerates Diabetic Wound Healing.

JID innovations : skin science from molecules to population health

Lin CW,Chen CC,Huang WY,Chen YY,Chen ST,Chou HW,Hung CM,Chen WJ,Lu CS,Nian SX,Chen SG,Chang HW,Chang VHS,Liu LY,Kuo ML,Chang SC

Published figure using CD163 monoclonal antibody (Product # 12-1631-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2022

Tumor cell-derived asymmetric dimethylarginine regulates macrophage functions and polarization.

Cancer cell international

Chen YL,Lowery AT,Lin S,Walker AM,Chen KE

Published figure using CD163 monoclonal antibody (Product # 12-1631-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2022

Exosomal AP000439.2 from clear cell renal cell carcinoma induces M2 macrophage polarization to promote tumor progression through activation of STAT3.

Cell communication and signaling : CCS

Shen T,Miao S,Zhou Y,Yi X,Xue S,Du B,Tang C,Qu L,Fu D,Jia R,He H

Published figure using CD163 monoclonal antibody (Product # 12-1631-82) in Flow Cytometry

Sat Sep 24 00:00:00 EDT 2022

Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy.

Cell reports

Wang W,Wu S,Cen Z,Zhang Y,Chen Y,Huang Y,Cillo AR,Prokopec JS,Quarato G,Vignali DAA,Stewart-Ornstein J,Li S,Lu B,Gong YN

Published figure using CD163 monoclonal antibody (Product # 12-1631-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

In mouse chronic pancreatitis CD25⁺FOXP3⁺ regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response.

Nature communications

Glaubitz J,Wilden A,Golchert J,Homuth G,Völker U,Bröker BM,Thiele T,Lerch MM,Mayerle J,Aghdassi AA,Weiss FU,Sendler M

12-1631-82 was used in Flow cytometry/Cell sorting to identify Treg cells as key regulators of the type-2 immune response and of organ remodeling during CP.

Wed Aug 03 00:00:00 EDT 2022

Tumour-associated macrophages drive stromal cell-dependent collagen crosslinking and stiffening to promote breast cancer aggression.

Nature materials

Maller O,Drain AP,Barrett AS,Borgquist S,Ruffell B,Zakharevich I,Pham TT,Gruosso T,Kuasne H,Lakins JN,Acerbi I,Barnes JM,Nemkov T,Chauhan A,Gruenberg J,Nasir A,Bjarnadottir O,Werb Z,Kabos P,Chen YY,Hwang ES,Park M,Coussens LM,Nelson AC,Hansen KC,Weaver VM

Published figure using CD163 monoclonal antibody (Product # 12-1631-82) in Immunohistochemistry

Thu Apr 01 00:00:00 EDT 2021

Exosomes derived from inflammatory myoblasts promote M1 polarization and break the balance of myoblast proliferation/differentiation.

World journal of stem cells

Luo ZW,Sun YY,Lin JR,Qi BJ,Chen JW

Published figure using CD163 monoclonal antibody (Product # 12-1631-82) in Flow Cytometry

Fri Nov 26 00:00:00 EST 2021

Pulmonary transplantation of alpha-1 antitrypsin (AAT)-transgenic macrophages provides a source of functional human AAT in vivo.

Gene therapy

Janosz E,Hetzel M,Spielmann H,Tumpara S,Rossdam C,Schwabbauer M,Kloos D,von Kaisenberg C,Schambach A,Buettner FFR,Janciauskiene S,Lachmann N,Moritz T

Published figure using CD163 monoclonal antibody (Product # 12-1631-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Magnoliae Cortex Alleviates Muscle Wasting by Modulating M2 Macrophages in a Cisplatin-Induced Sarcopenia Mouse Model.

International journal of molecular sciences

Hong M,Han IH,Choi I,Cha N,Kim W,Kim SK,Bae H

12-1631-82 was used in Flow cytometry/Cell sorting to investigate whether the oral administration of M.C could decrease cisplatin-induced sarcopenia by modulating M2 macrophage polarization in mice.

Sat Mar 20 00:00:00 EDT 2021

Magnoliae Cortex Alleviates Muscle Wasting by Modulating M2 Macrophages in a Cisplatin-Induced Sarcopenia Mouse Model.

International journal of molecular sciences

Hong M,Han IH,Choi I,Cha N,Kim W,Kim SK,Bae H

12-1631-82 was used in Flow cytometry/Cell sorting to investigate whether the oral administration of M.C could decrease cisplatin-induced sarcopenia by modulating M2 macrophage polarization in mice.

Sat Mar 20 00:00:00 EDT 2021

Convallatoxin Promotes M2 Macrophage Polarization to Attenuate Atherosclerosis Through PPARγ-Integrin α_vβ₅ Signaling Pathway.

Drug design, development and therapy

Zhang Y,Shi X,Han J,Peng W,Fang Z,Zhou Y,Xu X,Lin J,Xiao F,Zhao L,Lin Y

Published figure using CD163 monoclonal antibody (Product # 12-1631-82) in Flow Cytometry

Wed Oct 06 00:00:00 EDT 2021

IGFBP2 promotes immunosuppression associated with its mesenchymal induction and FcγRIIB phosphorylation in glioblastoma.

PloS one

Liu Y,Song C,Shen F,Zhang J,Song SW

12-1631-82 was used in Flow Cytometry to investigate the effect of IGFBP2 on GBM exposed immune cells and its association with the mesenchymal induction, using a syngeneic mouse GBM model, human GBM samples, and cancer-immune cell co-culture experiments.

Thu Apr 02 00:00:00 EDT 2020

LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8⁺ T cell tumor-infiltration impairing anti-PD1 therapy.

Nature communications

Pascual-García M,Bonfill-Teixidor E,Planas-Rigol E,Rubio-Perez C,Iurlaro R,Arias A,Cuartas I,Sala-Hojman A,Escudero L,Martínez-Ricarte F,Huber-Ruano I,Nuciforo P,Pedrosa L,Marques C,Braña I,Garralda E,Vieito M,Squatrito M,Pineda E,Graus F,Espejo C,Sahuquillo J,Tabernero J,Seoane J

12-1631-82 was used in Flow Cytometry to study the effect of the combination of LIF neutralising antibodies with the inhibition of the PD1 immune checkpoint on tumour progression.

Tue Jun 11 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: CD163; Scavenger receptor cysteine-rich type 1 protein M130; Soluble sCD163

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Gene Aliases: Cd163; CD163v2; CD163v3; M130

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UniProt ID: (Mouse) Q2VLH6

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Entrez Gene ID: (Mouse) 93671

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Function(s)

scavenger receptor activity serine protease

Process(es)

receptor-mediated endocytosis acute-phase response inflammatory response

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CD163 Monoclonal Antibody (TNKUPJ), PE, eBioscience™

CD163 Antibody (12-1631-82) in Flow

Product Details

12-1631-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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