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Invitrogen
CD15 Monoclonal Antibody (HI98), PerCP-eFluor™ 710, eBioscience™
Product Details
46-0159-42
Species Reactivity
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Product Specific Information
Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.
Applications Reported: This HI98 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HI98 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid-specific; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1
Gene Aliases: CD15; ELFT; FCT3A; FUC-TIV; FUT4; FUTIV; LeX; SSEA-1
UniProt ID: (Human) P22083
Entrez Gene ID: (Human) 2526
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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