Invitrogen

CD14 Monoclonal Antibody (Sa2-8), PE, eBioscience™

FIGURE: 1 / 28

CD14 Antibody (12-0141-82) in Flow

Staining of mouse thioglycolate-elicited peritoneal exudate cells with 0.25 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (blue histogram) or 0.25 µg of Anti-Mouse CD14 PE (purple histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

12-0141-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 34 publications 34 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Sa2-8

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465563

Product Specific Information

Description: The Sa2-8 monoclonal antibody reacts with mouse CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is a receptor for the complexes of LPS and LBP (LPS-Binding Protein) and is shown to associate with Toll-Like Receptor 4 (TLR4) and participate in the signaling and cellular response to bacterial LPS. In mouse, CD14 is expressed on the surface of macrophages and under certain conditions is also found in the serum in a secreted form. Sa2-8 has weak antagonistic activity (in NF-kappaB activation or TNF alpha production with LPS stimulation).

Applications Reported: The Sa2-8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This Sa2-8 antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Fasudil-modified macrophages reduce inflammation and regulate the immune response in experimental autoimmune encephalomyelitis.

Neural regeneration research

Liu C,Guo S,Liu R,Guo M,Wang Q,Chai Z,Xiao B,Ma C

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2024

Umbilical cord mesenchymal stem cell-derived apoptotic extracellular vesicles ameliorate cutaneous wound healing in type 2 diabetic mice via macrophage pyroptosis inhibition.

Stem cell research & therapy

Wang Y,Jing L,Lei X,Ma Z,Li B,Shi Y,Zhang W,Li Y,Zhou H,Hu K,Xue Y,Jin Y

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Tue Sep 19 00:00:00 EDT 2023

Optimization of differentiation and transcriptomic profile of THP-1 cells into macrophage by PMA.

PloS one

Liu T,Huang T,Li J,Li A,Li C,Huang X,Li D,Wang S,Liang M

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Wed Jul 19 00:00:00 EDT 2023

Macrophage polarization is involved in liver fibrosis induced by β ₁-adrenoceptor autoantibody.

Acta biochimica et biophysica Sinica

Wu Y,Fan X,Yu H,Liu J,Duan Y,Zhang S,Yan L,Du Y,Liu H

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Thu Aug 25 00:00:00 EDT 2022

Early activation of the cardiac CX3CL1/CX3CR1 axis delays β-adrenergic-induced heart failure.

Scientific reports

Flamant M,Mougenot N,Balse E,Le Fèvre L,Atassi F,Gautier EL,Le Goff W,Keck M,Nadaud S,Combadière C,Boissonnas A,Pavoine C

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Thu Sep 09 00:00:00 EDT 2021

CD14⁺CD16^- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK.

Arthritis research & therapy

Xue J,Xu L,Zhu H,Bai M,Li X,Zhao Z,Zhong H,Cheng G,Li X,Hu F,Su Y

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Mon Sep 21 00:00:00 EDT 2020

Evaluation of two methods to isolate Schwann cells from murine sciatic nerve.

Journal of neuroscience methods

Tomlinson JE,Golshadi M,Donahue CJ,Dong L,Cheetham J

12-0141 was used in Flow cytometry/Cell sorting to present two techniques to enrich schwann cells (SC) from other cell populations at the site of peripheral nerve injury.

Sat Feb 01 00:00:00 EST 2020

CNS Immune Profiling in a Dengue Virus-Infected Immunocompetent Outbred ICR Mice Strain.

Frontiers in cellular and infection microbiology

Shen TJ,Chen CL,Jhan MK,Tseng PC,Lin CF

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Thu Jun 17 00:00:00 EDT 2021

Immunoadjuvant Activity of Fucoidans from the Brown Alga Fucus evanescens.

Marine drugs

Kuznetsova TA,Smolina TP,Makarenkova ID,Ivanushko LA,Persiyanova EV,Ermakova SP,Silchenko AS,Zaporozhets TS,Besednova NN,Fedyanina LN,Kryzhanovsky SP

12-0141 was used in Flow cytometry/Cell sorting to comparatively evaluate the effect of fucoidans from the brown alga Fucus evanescens on effector functions of innate and adaptive immunity cells in vitro and in vivo.

Wed Mar 11 00:00:00 EDT 2020

IL-31 plays dual roles in lung inflammation in an OVA-induced murine asthma model.

Biology open

Huang J,Yue H,Jiang T,Gao J,Shi Y,Shi B,Wu X,Gou X

12-0141 was used in Flow cytometry/Cell sorting to investigate the dynamic expression of interleukin 31 (IL-31) and IL-31RA during eight consecutive ovalbumin challenges and measure the chemokines from lung alveolar epithelial cells induced by IL-31.

Tue Jan 15 00:00:00 EST 2019

Leucine-rich repeats and calponin homology containing 4 (Lrch4) regulates the innate immune response.

The Journal of biological chemistry

Aloor JJ,Azzam KM,Guardiola JJ,Gowdy KM,Madenspacher JH,Gabor KA,Mueller GA,Lin WC,Lowe JM,Gruzdev A,Henderson MW,Draper DW,Merrick BA,Fessler MB

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Fri Feb 08 00:00:00 EST 2019

Chanzyme TRPM7 Mediates the Ca²⁺ Influx Essential for Lipopolysaccharide-Induced Toll-Like Receptor 4 Endocytosis and Macrophage Activation.

Immunity

Schappe MS,Szteyn K,Stremska ME,Mendu SK,Downs TK,Seegren PV,Mahoney MA,Dixit S,Krupa JK,Stipes EJ,Rogers JS,Adamson SE,Leitinger N,Desai BN

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Tue Jan 16 00:00:00 EST 2018

Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages.

Breast cancer research : BCR

Donzelli S,Milano E,Pruszko M,Sacconi A,Masciarelli S,Iosue I,Melucci E,Gallo E,Terrenato I,Mottolese M,Zylicz M,Zylicz A,Fazi F,Blandino G,Fontemaggi G

12-0141 was used in Flow cytometry/Cell sorting to investigate whether ID4 exerts its pro-angiogenic function while also modulating the activity of tumour-associated macrophages in breast cancer.

Tue Jun 19 00:00:00 EDT 2018

TIM‑4 blockade of KCs combined with exogenous TGF‑β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts.

International journal of molecular medicine

Wu H,Xu X,Li J,Gong J,Li M

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2018

MicroRNA-10a/b are regulators of myeloid differentiation and acute myeloid leukemia.

Oncology letters

Bi L,Sun L,Jin Z,Zhang S,Shen Z

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Butyrate upregulates the TLR4 expression and the phosphorylation of MAPKs and NK-κB in colon cancer cell in vitro.

Oncology letters

Xiao T,Wu S,Yan C,Zhao C,Jin H,Yan N,Xu J,Wu Y,Li C,Shao Q,Xia S

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.

Immunity

Kishore M,Cheung KCP,Fu H,Bonacina F,Wang G,Coe D,Ward EJ,Colamatteo A,Jangani M,Baragetti A,Matarese G,Smith DM,Haas R,Mauro C,Wraith DC,Okkenhaug K,Catapano AL,De Rosa V,Norata GD,Marelli-Berg FM

12-0141 was used in Flow cytometry/Cell sorting to demonstrate that glucokinase-dependent glycolysis regulates regulatory T cell migration.

Tue Nov 21 00:00:00 EST 2017

Construction of developmental lineage relationships in the mouse mammary gland by single-cell RNA profiling.

Nature communications

Pal B,Chen Y,Vaillant F,Jamieson P,Gordon L,Rios AC,Wilcox S,Fu N,Liu KH,Jackling FC,Davis MJ,Lindeman GJ,Smyth GK,Visvader JE

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Mon Nov 20 00:00:00 EST 2017

Alternatively activated macrophages determine repair of the infarcted adult murine heart.

The Journal of clinical investigation

Shiraishi M,Shintani Y,Shintani Y,Ishida H,Saba R,Yamaguchi A,Adachi H,Yashiro K,Suzuki K

12-0141 was used in Immunocytochemistry to study repair of the infarcted adult murine heart determined by alternatively activated macrophages.

Wed Jun 01 00:00:00 EDT 2016

T3 Regulates a Human Macrophage-Derived TSH-β Splice Variant: Implications for Human Bone Biology.

Endocrinology

Baliram R,Latif R,Morshed SA,Zaidi M,Davies TF

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

Model-Based Characterization of Inflammatory Gene Expression Patterns of Activated Macrophages.

PLoS computational biology

Rex J,Albrecht U,Ehlting C,Thomas M,Zanger UM,Sawodny O,Häussinger D,Ederer M,Feuer R,Bode JG

12-0141 was used in Flow cytometry/Cell sorting to investigate inflammatory gene expression patterns of activated macrophages via model-based characterization.

Fri Jul 01 00:00:00 EDT 2016

Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.

Stem cell reports

Mucci A,Kunkiel J,Suzuki T,Brennig S,Glage S,Kühnel MP,Ackermann M,Happle C,Kuhn A,Schambach A,Trapnell BC,Hansen G,Moritz T,Lachmann N

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Mechanisms of Toll-like Receptor 4 Endocytosis Reveal a Common Immune-Evasion Strategy Used by Pathogenic and Commensal Bacteria.

Immunity

Tan Y,Zanoni I,Cullen TW,Goodman AL,Kagan JC

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Tue Nov 17 00:00:00 EST 2015

Role of caveolin-3 in lymphocyte activation.

Life sciences

Tran C,Stary CM,Schilling JM,Bentley B,Patel HH,Roth DM

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Thu Jan 15 00:00:00 EST 2015

EPA and DHA exposure alters the inflammatory response but not the surface expression of Toll-like receptor 4 in macrophages.

Lipids

Honda KL,Lamon-Fava S,Matthan NR,Wu D,Lichtenstein AH

12-0141 was used in Flow cytometry/Cell sorting to investigate the influence of membrane fatty acid profile on TLR4-mediated inflammation in RAW 264.7 macrophages.

Sun Feb 01 00:00:00 EST 2015

CD326(lo)CD103(lo)CD11b(lo) dermal dendritic cells are activated by thymic stromal lymphopoietin during contact sensitization in mice.

Journal of immunology (Baltimore, Md. : 1950)

Ochiai S,Roediger B,Abtin A,Shklovskaya E,Fazekas de St Groth B,Yamane H,Weninger W,Le Gros G,Ronchese F

12014182 was used in flow cytometry to show that CD326(lo)CD103(lo)CD11b(lo) dermal dendritic cells respond to thymic stromal lymphopoietin

Mon Sep 01 00:00:00 EDT 2014

The monocyte to macrophage transition in the murine sterile wound.

PloS one

Crane MJ,Daley JM,van Houtte O,Brancato SK,Henry WL,Albina JE

12-0141 was used in Flow cytometry/Cell sorting to examine the origin of wound repair macrophages in sterile wounds using the subcutaneous polyvinyl alcohol sponge implantation model in mice.

Tue Nov 04 00:00:00 EST 2014

CD4+ CD25+ FOXP3+ Treg cells induced by rSSP4 derived from T. cruzi amastigotes increase parasitemia in an experimental Chagas disease model.

BioMed research international

Flores-García Y,Rosales-Encina JL,Rosales-García VH,Satoskar AR,Talamás-Rohana P

12-0141 was used in Flow cytometry/Cell sorting to examine the degree of benefit conferred by Treg cells during T.cruzi infection.

Tue Nov 12 00:00:00 EST 2013

SHARPIN is essential for cytokine production, NF-κB signaling, and induction of Th1 differentiation by dendritic cells.

PloS one

Wang Z,Sokolovska A,Seymour R,Sundberg JP,Hogenesch H

12-0141 was used in Flow cytometry/Cell sorting to demonstrate that loss of the Sharpin gene in mice significantly affects the immune function of dendritic cells.

Tue Jul 31 00:00:00 EDT 2012

Impact of enzymatic tissue disintegration on the level of surface molecule expression and immune cell function.

European journal of microbiology & immunology

Autengruber A,Gereke M,Hansen G,Hennig C,Bruder D

12-0141 was used in Flow cytometry/Cell sorting to indicate that enzymatic tissue disintegration can have profound effects on the expression of a variety of cell-surface molecules.

Fri Jun 01 00:00:00 EDT 2012

Multinucleated giant cells from fibroblast cultures.

Biomaterials

Holt DJ,Grainger DW

12-0141 was used in Immunofluorescence to investigate giant multinucleated cells.

Wed Jun 01 00:00:00 EDT 2011

Cellular effectors mediating Th17-dependent clearance of pneumococcal colonization in mice.

The Journal of clinical investigation

Zhang Z,Clarke TB,Weiser JN

12-0141 was used in Flow cytometry/Cell sorting to demonstrate that monocytes/macrophages and neutrophils recruited to the upper airway lumen are key effectors in clearing primary and secondary Streptococcal pneumoniae colonisation, respectively.

Wed Jul 01 00:00:00 EDT 2009

An adventitial IL-6/MCP1 amplification loop accelerates macrophage-mediated vascular inflammation leading to aortic dissection in mice.

The Journal of clinical investigation

Tieu BC,Lee C,Sun H,Lejeune W,Recinos A,Ju X,Spratt H,Guo DC,Milewicz D,Tilton RG,Brasier AR

12-0141 was used in Flow cytometry/Cell sorting to identify a vascular inflammatory pathway that contributes to the development of cardiovascular disease.

Tue Dec 01 00:00:00 EST 2009

Immune depletion with cellular mobilization imparts immunoregulation and reverses autoimmune diabetes in nonobese diabetic mice.

Diabetes

Parker MJ,Xue S,Alexander JJ,Wasserfall CH,Campbell-Thompson ML,Battaglia M,Gregori S,Mathews CE,Song S,Troutt M,Eisenbeis S,Williams J,Schatz DA,Haller MJ,Atkinson MA

12-0141 was used in Flow cytometry/Cell sorting to investigate the use of ATG and GCSF in therapies to reverse overt hyperglycaemia in a diabetic mouse model.

Thu Oct 01 00:00:00 EDT 2009

cAMP modulates macrophage development by suppressing M-CSF-induced MAPKs activation.

Cellular & molecular immunology

Zhu N,Cui J,Qiao C,Li Y,Ma Y,Zhang J,Shen B

12-0141 was used in Flow cytometry/Cell sorting to explore the effects of cAMP on macrophage development using a model of bone marrow-derived murine macrophages.

Tue Apr 01 00:00:00 EDT 2008

Circadian mechanisms in murine and human bone marrow mesenchymal stem cells following dexamethasone exposure.

Bone

Wu X,Yu G,Parks H,Hebert T,Goh BC,Dietrich MA,Pelled G,Izadpanah R,Gazit D,Bunnell BA,Gimble JM

Published figure using CD14 monoclonal antibody (Product # 12-0141-82) in Flow Cytometry

Thu May 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

View more View less

Gene Aliases: Cd14

View more View less

UniProt ID: (Mouse) P10810

View more View less

Entrez Gene ID: (Mouse) 12475

View more View less

Function(s)

lipopolysaccharide binding lipoteichoic acid binding transmembrane signal receptor

Process(es)

response to molecule of bacterial origin immune system process inflammatory response positive regulation of tumor necrosis factor production response to tumor necrosis factor innate immune response positive regulation of endocytosis positive regulation of cytokine secretion cellular response to lipopolysaccharide cellular response to lipoteichoic acid

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD14 Monoclonal Antibody (Sa2-8), PE, eBioscience™

CD14 Antibody (12-0141-82) in Flow

Product Details

12-0141-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!