Invitrogen

CD14 Recombinant Rabbit Monoclonal Antibody (RM415)

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This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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Datasheet

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Datasheet

Protocols

Questions & Answers

CD14 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD14 Antibody (MA5-36075) in IHC (P)

Immunohistochemical analysis of CD14 was performed using formalin-fixed paraffin-embedded human human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes... View More

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CD14 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of CD14 was observed in THP-1 differentiated to M1 macrophage and THP-1 differentiated to M1 macrophage followed by lipopolysaccharide treatment and not in Jurkat, Raji and MOLT-4 which are reported to be negative as observed in Fig (a). In Fig (b), tissue expression of CD14 was observed only in mouse axillary lymph node in comparison to mouse skeletal muscle and mouse heart using CD14 Recombinant Rabbit Monoclonal Antibody (RM415) (Product # MA5-36075) in western blot. {RE}

Product Details

MA5-36075

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM415

Immunogen

A peptide corresponding to the C-terminus of human CD14

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.05 mg/mL

Purification

Protein A

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2866687

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

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Gene Aliases: CD14

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UniProt ID: (Human) P08571, (Mouse) P10810

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Entrez Gene ID: (Human) 929, (Mouse) 12475

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Function(s)

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding transmembrane signal receptor

Process(es)

MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway receptor-mediated endocytosis phagocytosis apoptotic process inflammatory response cell surface receptor signaling pathway I-kappaB kinase/NF-kappaB signaling response to heat lipopolysaccharide-mediated signaling pathway response to magnesium ion positive regulation of type I interferon production positive regulation of interferon-gamma production positive regulation of tumor necrosis factor production negative regulation of MyD88-independent toll-like receptor signaling pathway toll-like receptor 4 signaling pathway response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway innate immune response response to ethanol positive regulation of endocytosis positive regulation of cytokine secretion response to electrical stimulus necroptotic process cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide apoptotic signaling pathway response to molecule of bacterial origin immune system process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD14 Recombinant Rabbit Monoclonal Antibody (RM415)

CD14 Antibody (MA5-36075) in IHC (P)

Product Details

MA5-36075

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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