Invitrogen

CD137 Monoclonal Antibody (BBK-2)

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CD137 Antibody (MA5-13739) in Flow

Flow cytometry analysis of CD137 in PBMC cells stimulated with PHA for 3 days (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-13739) at a dilution of 1 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by... View More

CD137 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

MA5-13739

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

2 µg/mL

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 2 publications 2 publications

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

BBK-2

Immunogen

Ectodomain of human 4-1BB recombinant protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10981098

Product Specific Information

MA5-13739 targets CD137 in FACS and IF applications and shows reactivity with Human samples.

The MA5-13739 immunogen is ectodomain of human 4-1BB recombinant protein.

Target Information

CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CD137 signaling in Hodgkin and Reed-Sternberg cell lines induces IL-13 secretion, immune deviation and enhanced growth.

Oncoimmunology

Rajendran S,Ho WT,Schwarz H

MA5-13739 was used in Immunohistochemistry to validate CD137 as a candidate target for immunotherapy of HL.

Wed Jun 01 00:00:00 EDT 2016

Accumulation of 4-1BBL+ B cells in the elderly induces the generation of granzyme-B+ CD8+ T cells with potential antitumor activity.

Blood

Lee-Chang C,Bodogai M,Moritoh K,Olkhanud PB,Chan AC,Croft M,Mattison JA,Holst PJ,Gress RE,Ferrucci L,Hakim F,Biragyn A

MA5-13739 was used in Functional assay to propose that 4BL cells and the 4-1BBL signaling pathway are useful targets for improved effectiveness of natural antitumor defenses and therapeutic immune manipulations in the elderly.

Thu Aug 28 00:00:00 EDT 2014

Expression of CD137 on Hodgkin and Reed-Sternberg cells inhibits T-cell activation by eliminating CD137 ligand expression.

Cancer research

Ho WT,Pang WL,Chong SM,Castella A,Al-Salam S,Tan TE,Moh MC,Koh LK,Gan SU,Cheng CK,Schwarz H

MA5-13739 was used in immunohistochemistry to study the role of CD137 on Hodgkin and Reed-Sternberg cells in the mechanism by which they inhibit the activation of T-cells

Tue Jan 15 00:00:00 EST 2013

CD137 is expressed in follicular dendritic cell tumors and in classical Hodgkin and T-cell lymphomas: diagnostic and therapeutic implications.

The American journal of pathology

Anderson MW,Zhao S,Freud AG,Czerwinski DK,Kohrt H,Alizadeh AA,Houot R,Azambuja D,Biasoli I,Morais JC,Spector N,Molina-Kirsch HF,Warnke RA,Levy R,Natkunam Y

MA5-13739 was used in immunohistochemistry to study the diagnostic and therapeutic importance of CD137 expression by follicular dendritic cell tumors and Hodgkin and T-cell lymphomas

Sat Sep 01 00:00:00 EDT 2012

Prediction of survival in diffuse large B-cell lymphoma based on the expression of 2 genes reflecting tumor and microenvironment.

Blood

Alizadeh AA,Gentles AJ,Alencar AJ,Liu CL,Kohrt HE,Houot R,Goldstein MJ,Zhao S,Natkunam Y,Advani RH,Gascoyne RD,Briones J,Tibshirani RJ,Myklebust JH,Plevritis SK,Lossos IS,Levy R

MA5-13739 was used in immunohistochemistry to study the expression of genes reflecting tumor and microenvironment and their value in predicting survival in diffuse large B-cell lymphoma

Thu Aug 04 00:00:00 EDT 2011

CD137 ligand mediates opposite effects in human and mouse NK cells and impairs NK-cell reactivity against human acute myeloid leukemia cells.

Blood

Baessler T,Charton JE,Schmiedel BJ,Grünebach F,Krusch M,Wacker A,Rammensee HG,Salih HR

MA5-13739 was used in flow cytometry to study the role of CD137 ligand in NK-cell reactivity against human acute myeloid leukemia cells

Thu Apr 15 00:00:00 EDT 2010

CD137 enhances monocyte-ICAM-1 interactions in an E-selectin-dependent manner under flow conditions.

Molecular immunology

Quek BZ,Lim YC,Lin JH,Tan TE,Chan J,Biswas A,Schwarz H

MA5-13739 was used in flow cytometry to study the role of CD137 in monocyte recruitment to inflammation

Sat May 01 00:00:00 EDT 2010

Induction of proliferation and monocytic differentiation of human CD34+ cells by CD137 ligand signaling.

Stem cells (Dayton, Ohio)

Jiang D,Yue PS,Drenkard D,Schwarz H

MA5-13739 was used in immunohistochemistry to investigate the influence of CD137 on CD34+ cell amplification and differentiation

Mon Sep 01 00:00:00 EDT 2008

Molecular mechanisms and gene regulation of melphalan- and hyperthermia-induced apoptosis in Ewing sarcoma cells.

Anticancer research

Krause C,Klüttermann K,Mauz-Körholz C

MA5-13739 was used in blocking or activating experiment to study the mechanisms underlying melphalan- and hyperthermia-induced apoptosis in Ewing sarcoma cells

Tue Dec 16 00:00:00 EST 2008

Bioinformatics

Protein Aliases: 4 1 BB; 4-1BB ligand receptor; 41BB; CD137; CD137 antigen; CDw137; homolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; s4 1 BB; s4 1BB; s41BB; sCD137; soluble 4 1 BB; soluble 4 1BB; soluble 41BB; soluble CD137; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; Tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9

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Gene Aliases: 4-1BB; CD137; CDw137; ILA; TNFRSF9

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UniProt ID: (Human) Q07011

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Entrez Gene ID: (Human) 3604

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Function(s)

receptor activity tumor necrosis factor-activated receptor activity cytokine binding transmembrane signal receptor

Process(es)

apoptotic process inflammatory response immune response multicellular organism development negative regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of apoptotic process protein homotrimerization negative regulation of interleukin-10 secretion negative regulation of interleukin-12 secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD137 Monoclonal Antibody (BBK-2)

CD137 Antibody (MA5-13739) in Flow

Product Details

MA5-13739

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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