Invitrogen

CD134 (OX40) Monoclonal Antibody (OX-86), eBioscience™

CD134 (OX40) Antibody in Flow Cytometry (Flow)

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CD134 (OX40) Antibody (14-1341-82) in Flow

Staining of 3-day unstimulated (left) and 3-day Con A-stimulated (right) BALB/c splenocytes with 0.25 µg of Rat IgG1 K Isotype Control Purified (Product # 14-4301-82) (open histogram) or 0.25 µg of Anti-Mouse CD134 (OX40) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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Product Details

14-1341-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 12 publications 12 publications

Neutralization (Neu)

Assay-Dependent

Functional Assay (Functional)

Assay-Dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

OX-86

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467476

Product Specific Information

Description: The OX-86 monoclonal antibody reacts with mouse CD134, also known as OX40. A member of the TNF receptor superfamily, CD134 is a 50 kDa type I membrane glycoprotein expressed by activated mouse T lymphocytes. Rat CD134 was initially identified as an activation marker only on activated CD4+ T cells. In contrast, mouse CD134 is expressed by both activated CD4+ and CD8+ T cells. The interaction of CD134 with CD252 (OX40 ligand) has been implicated in T cell-dependent humoral responses, regulation of primary T cell expansion, survival of T cells, size of the memory T cell pool, and regulation of tolerance in the CD4+ T cell compartment.

Applications Reported: The OX-86 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It does not block binding of CD134 to its ligand, but has been reported for use in functional assays. Please use Functional Grade purified OX-86 in functional assays.

Applications Tested: The OX-86 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

OX40 is a protein receptor found on the surface of T cells, a type of white blood cell that plays a crucial role in the immune system. When activated, OX40 promotes the proliferation and survival of T cells, leading to a stronger immune response against cancer cells and infectious agents. OX40 agonists, which are drugs that activate OX40, have shown promising results in preclinical and clinical studies as a potential immunotherapy for cancer. They can enhance the efficacy of other cancer treatments such as chemotherapy and checkpoint inhibitors, and may also have applications in autoimmune diseases and allergies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections.

Immunity

Harb H,Benamar M,Lai PS,Contini P,Griffith JW,Crestani E,Schmitz-Abe K,Chen Q,Fong J,Marri L,Filaci G,Del Zotto G,Pishesha N,Kolifrath S,Broggi A,Ghosh S,Gelmez MY,Oktelik FB,Cetin EA,Kiykim A,Kose M,Wang Z,Cui Y,Yu XG,Li JZ,Berra L,Stephen-Victor E,Charbonnier LM,Zanoni I,Ploegh H,Deniz G,De Palma R,Chatila TA

Published figure using CD134 (OX40) monoclonal antibody (Product # 14-1341-82) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity.

Cancer cell

Hutton C,Heider F,Blanco-Gomez A,Banyard A,Kononov A,Zhang X,Karim S,Paulus-Hock V,Watt D,Steele N,Kemp S,Hogg EKJ,Kelly J,Jackstadt RF,Lopes F,Menotti M,Chisholm L,Lamarca A,Valle J,Sansom OJ,Springer C,Malliri A,Marais R,Pasca di Magliano M,Zelenay S,Morton JP,Jørgensen C

14-1341-82 was used in Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Neutralization, Western Blot to reveal two functionally distinct pancreatic fibroblast lineages and highlight the importance of mesenchymal and immune cell interactions in restricting tumor growth.

Mon Sep 13 00:00:00 EDT 2021

Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia.

Oncoimmunology

Stokes J,Hoffman EA,Molina MS,Kummet N,Simpson RJ,Zeng Y,Katsanis E

Published figure using CD134 (OX40) monoclonal antibody (Product # 14-1341-82) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Anti-PD-L1 Efficacy Can Be Enhanced by Inhibition of Myeloid-Derived Suppressor Cells with a Selective Inhibitor of PI3Kδ/γ.

Cancer research

Davis RJ,Moore EC,Clavijo PE,Friedman J,Cash H,Chen Z,Silvin C,Van Waes C,Allen C

14-1341 was used in Flow cytometry/Cell sorting to demonstrate functional inhibition of myeloid-derived suppressor cells with IPI-145 which enhances responses to PD-L1 blockade.

Mon May 15 00:00:00 EDT 2017

NFκB-Pim-1-Eomesodermin axis is critical for maintaining CD8 T-cell memory quality.

Proceedings of the National Academy of Sciences of the United States of America

Knudson KM,Pritzl CJ,Saxena V,Altman A,Daniels MA,Teixeiro E

14-1341 was used in Flow cytometry/Cell sorting to show that TCR/antigenic signals regulate the induction of a NFκB/Pim-1 feedback loop that controls expression of Eomes and thereby long-term maintenance of the memory pool.

Tue Feb 28 00:00:00 EST 2017

The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice.

Journal of immunology (Baltimore, Md. : 1950)

Sitrin J,Suto E,Wuster A,Eastham-Anderson J,Kim JM,Austin CD,Lee WP,Behrens TW

Published figure using CD134 (OX40) monoclonal antibody (Product # 14-1341-82) in Flow Cytometry

Tue Aug 15 00:00:00 EDT 2017

OX40- and CD27-mediated costimulation synergizes with anti-PD-L1 blockade by forcing exhausted CD8+ T cells to exit quiescence.

Journal of immunology (Baltimore, Md. : 1950)

Buchan S,Manzo T,Flutter B,Rogel A,Edwards N,Zhang L,Sivakumaran S,Ghorashian S,Carpenter B,Bennett C,Freeman GJ,Sykes M,Croft M,Al-Shamkhani A,Chakraverty R

14-1341 was used in Flow cytometry/Cell sorting to study how CD27-mediated co-stimulation can synergise with co-inhibitory checkpoint blockade to switch off molecular programs for quiescence in exhausted T cell populations.

Thu Jan 01 00:00:00 EST 2015

Pak2 Links TCR Signaling Strength to the Development of Regulatory T Cells and Maintains Peripheral Tolerance.

Journal of immunology (Baltimore, Md. : 1950)

O'Hagan KL,Choi J,Pryshchep O,Chernoff J,Phee H

14-1341 was used in Flow cytometry/Cell sorting to show a novel role for the actin cytoskeletal remodeling protein, p21-activated kinase 2, in Treg development and homeostasis.

Sat Aug 15 00:00:00 EDT 2015

Roquin paralogs 1 and 2 redundantly repress the Icos and Ox40 costimulator mRNAs and control follicular helper T cell differentiation.

Immunity

Vogel KU,Edelmann SL,Jeltsch KM,Bertossi A,Heger K,Heinz GA,Zöller J,Warth SC,Hoefig KP,Lohs C,Neff F,Kremmer E,Schick J,Repsilber D,Geerlof A,Blum H,Wurst W,Heikenwälder M,Schmidt-Supprian M,Heissmeyer V

14-1341 was used in Immunoprecipitation to suggest that Roquin-1 and -2 maintain tolerance by preventing inappropriate T cell activation and Tfh cell differentiation.

Thu Apr 18 00:00:00 EDT 2013

OX40L/Jagged1 cosignaling by GM-CSF-induced bone marrow-derived dendritic cells is required for the expansion of functional regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Gopisetty A,Bhattacharya P,Haddad C,Bruno JC,Vasu C,Miele L,Prabhakar BS

14-1341 was used in Functional assays to show that OX40L induced signalling was essential but not sufficient for GM-BMDC mediated Treg expansion, and requires additional molecular interactions.

Sat Jun 01 00:00:00 EDT 2013

Transcutaneous vaccination via laser microporation.

Journal of controlled release : official journal of the Controlled Release Society

Weiss R,Hessenberger M,Kitzmüller S,Bach D,Weinberger EE,Krautgartner WD,Hauser-Kronberger C,Malissen B,Boehler C,Kalia YN,Thalhamer J,Scheiblhofer S

14-1341 was used in Flow cytometry/Cell sorting to investigate the potential of transcutaneous immunisation via laser-generated micropores for induction of specific immune responses.

Mon Sep 10 00:00:00 EDT 2012

IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE).

PloS one

Rynda A,Maddaloni M,Ochoa-Repáraz J,Callis G,Pascual DW

14-1341 was used in Flow cytometry/Cell sorting to demonstrate the potential for IL-28 as an alternative therapy for EAE.

Thu Jan 14 00:00:00 EST 2010

NKT cell-plasmacytoid dendritic cell cooperation via OX40 controls viral infection in a tissue-specific manner.

Immunity

Diana J,Griseri T,Lagaye S,Beaudoin L,Autrusseau E,Gautron AS,Tomkiewicz C,Herbelin A,Barouki R,von Herrath M,Dalod M,Lehuen A

14-1341 was used in Flow cytometry/Cell sorting to investigate how pDCs act as an essential partner of iNKT cells for mounting an efficient, nondeleterious antiviral response in peripheral tissue.

Fri Feb 20 00:00:00 EST 2009

GITR triggering induces expansion of both effector and regulatory CD4+ T cells in vivo.

Journal of immunology (Baltimore, Md. : 1950)

van Olffen RW,Koning N,van Gisbergen KP,Wensveen FM,Hoek RM,Boon L,Hamann J,van Lier RA,Nolte MA

14-1341 was used in Flow cytometry/Cell sorting to study the role of glucocorticoid-induced TNF receptor family-related protein on activated and regulatory T cells.

Mon Jun 15 00:00:00 EDT 2009

Adenovirus vector-mediated in vivo gene transfer of OX40 ligand to tumor cells enhances antitumor immunity of tumor-bearing hosts.

Cancer research

Andarini S,Kikuchi T,Nukiwa M,Pradono P,Suzuki T,Ohkouchi S,Inoue A,Maemondo M,Ishii N,Saijo Y,Sugamura K,Nukiwa T

Sat May 01 00:00:00 EDT 2004

Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an OX40 ligand-IgG fusion protein.

Journal of immunology (Baltimore, Md. : 1950)

Higgins LM,McDonald SA,Whittle N,Crockett N,Shields JG,MacDonald TT

Fri Jan 01 00:00:00 EST 1999

Bioinformatics

Protein Aliases: CD134; OX40 antigen; OX40L receptor; tax-transcriptionally activated glycoprotein 1; Tumor necrosis factor receptor superfamily member 4

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Gene Aliases: ACT35; CD134; Ly-70; Ox40; Tnfrsf4; Txgp1; TXGP1L

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UniProt ID: (Mouse) P47741

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Entrez Gene ID: (Mouse) 22163

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Function(s)

tumor necrosis factor-activated receptor activity protein binding transmembrane signal receptor

Process(es)

inflammatory response cellular defense response programmed cell death positive regulation of B cell proliferation tumor necrosis factor-mediated signaling pathway T cell proliferation regulation of apoptotic process negative regulation of sequence-specific DNA binding transcription factor activity regulation of protein kinase activity negative regulation of transcription, DNA-templated negative regulation of cytokine secretion positive regulation of immunoglobulin secretion negative regulation of activation-induced cell death of T cells negative regulation of extrinsic apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD134 (OX40) Monoclonal Antibody (OX-86), eBioscience™

CD134 (OX40) Antibody (14-1341-82) in Flow

Product Details

14-1341-82

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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