Invitrogen

CD134 (OX40) Monoclonal Antibody (OX-86), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

14 References

View all (84) OX40 (CD134) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD134 (OX40) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD134 (OX40) Antibody (12-1341-82) in Flow

Staining of unstimulated (left) or 3-day Con A-stimulated C57Bl/6 mouse splenocytes (right) with 0.25 µg of Rat IgG1 K Isotype Control PE (Product # 12-4301-82) (blue histogram) or 0.25 µg of Anti-Mouse CD134 (OX40) PE (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

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Product Details

12-1341-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OX-86

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465854

Product Specific Information

Description: The OX-86 monoclonal antibody reacts with mouse CD134, also known as OX40. A member of the TNF receptor superfamily, CD134 is a 50 kDa type I membrane glycoprotein expressed by activated mouse T lymphocytes. Rat CD134 was initially identified as an activation marker only on activated CD4+ T cells. In contrast, mouse CD134 is expressed by both activated CD4+ and CD8+ T cells. The interaction of CD134 with CD252 (OX40 ligand) has been implicated in T cell-dependent humoral responses, regulation of primary T cell expansion, survival of T cells, size of the memory T cell pool, and regulation of tolerance in the CD4+ T cell compartment.

Applications Reported: The OX-86 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The OX-86 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

OX40 is a protein receptor found on the surface of T cells, a type of white blood cell that plays a crucial role in the immune system. When activated, OX40 promotes the proliferation and survival of T cells, leading to a stronger immune response against cancer cells and infectious agents. OX40 agonists, which are drugs that activate OX40, have shown promising results in preclinical and clinical studies as a potential immunotherapy for cancer. They can enhance the efficacy of other cancer treatments such as chemotherapy and checkpoint inhibitors, and may also have applications in autoimmune diseases and allergies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections.

Immunity

Harb H,Benamar M,Lai PS,Contini P,Griffith JW,Crestani E,Schmitz-Abe K,Chen Q,Fong J,Marri L,Filaci G,Del Zotto G,Pishesha N,Kolifrath S,Broggi A,Ghosh S,Gelmez MY,Oktelik FB,Cetin EA,Kiykim A,Kose M,Wang Z,Cui Y,Yu XG,Li JZ,Berra L,Stephen-Victor E,Charbonnier LM,Zanoni I,Ploegh H,Deniz G,De Palma R,Chatila TA

Published figure using CD134 (OX40) monoclonal antibody (Product # 12-1341-82) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia.

Oncoimmunology

Stokes J,Hoffman EA,Molina MS,Kummet N,Simpson RJ,Zeng Y,Katsanis E

Published figure using CD134 (OX40) monoclonal antibody (Product # 12-1341-82) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

CD160 serves as a negative regulator of NKT cells in acute hepatic injury.

Nature communications

Kim TJ,Park G,Kim J,Lim SA,Kim J,Im K,Shin MH,Fu YX,Del Rio ML,Rodriguez-Barbosa JI,Yee C,Suh KS,Kim SJ,Ha SJ,Lee KM

12-1341-82 was used in Flow cytometry/Cell sorting to show that CD160 is not essential for NKT cell development.

Mon Jul 22 00:00:00 EDT 2019

PD-1 Blockade and CD27 Stimulation Activate Distinct Transcriptional Programs That Synergize for CD8⁺ T-Cell-Driven Antitumor Immunity.

Clinical cancer research : an official journal of the American Association for Cancer Research

Buchan SL,Fallatah M,Thirdborough SM,Taraban VY,Rogel A,Thomas LJ,Penfold CA,He LZ,Curran MA,Keler T,Al-Shamkhani A

12-1341 was used in Flow cytometry/Cell sorting to show that suboptimal T-cell invigoration in cancer patients undergoing treatment with PD-1 checkpoint blockers will be improved by dual PD-1 blockade and CD27 agonism.

Tue May 15 00:00:00 EDT 2018

Roquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation.

Nature communications

Essig K,Kronbeck N,Guimaraes JC,Lohs C,Schlundt A,Hoffmann A,Behrens G,Brenner S,Kowalska J,Lopez-Rodriguez C,Jemielity J,Holtmann H,Reiche K,Hackermüller J,Sattler M,Zavolan M,Heissmeyer V

12-1341 was used in Flow cytometry/Cell sorting to study how the robustness and magnitude of Roquin-mediated regulation is encoded in complex cis-elements.

Wed Sep 19 00:00:00 EDT 2018

The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice.

Journal of immunology (Baltimore, Md. : 1950)

Sitrin J,Suto E,Wuster A,Eastham-Anderson J,Kim JM,Austin CD,Lee WP,Behrens TW

Published figure using CD134 (OX40) monoclonal antibody (Product # 12-1341-82) in Flow Cytometry

Tue Aug 15 00:00:00 EDT 2017

Age-dependent divergent effects of OX40L treatment on the development of diabetes in NOD mice.

Autoimmunity

Haddad CS,Bhattacharya P,Alharshawi K,Marinelarena A,Kumar P,El-Sayed O,Elshabrawy HA,Epstein AL,Prabhakar BS

Published figure using CD134 (OX40) monoclonal antibody (Product # 12-1341-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Roquin recognizes a non-canonical hexaloop structure in the 3'-UTR of Ox40.

Nature communications

Janowski R,Heinz GA,Schlundt A,Wommelsdorf N,Brenner S,Gruber AR,Blank M,Buch T,Buhmann R,Zavolan M,Niessing D,Heissmeyer V,Sattler M

12-1341 was used in Flow cytometry/Cell sorting to reveal an unexpected recognition of hexaloop cis elements for the posttranscriptional regulation of target messenger RNAs by Roquin.

Thu Mar 24 00:00:00 EDT 2016

Triclosan Induces Thymic Stromal Lymphopoietin in Skin Promoting Th2 Allergic Responses.

Toxicological sciences : an official journal of the Society of Toxicology

Marshall NB,Lukomska E,Long CM,Kashon ML,Sharpnack DD,Nayak AP,Anderson KL,Jean Meade B,Anderson SE

12-1341 was used in Flow cytometry/Cell sorting to examine the effects of dermal triclosan exposure on the skin and lymph nodes of mice and in a human skin model to identify mechanisms for augmenting allergic responses.

Tue Sep 01 00:00:00 EDT 2015

Donor B cells in transplants augment clonal expansion and survival of pathogenic CD4+ T cells that mediate autoimmune-like chronic graft-versus-host disease.

Journal of immunology (Baltimore, Md. : 1950)

Young JS,Wu T,Chen Y,Zhao D,Liu H,Yi T,Johnston H,Racine J,Li X,Wang A,Todorov I,Zeng D

12-1341 was used in Flow cytometry/Cell sorting to investigate the mechanisms whereby donor B cells augment chronic graft-versus-host disease (cGVHD) pathogenesis, showing that B cells augment clonal expansion and survival of pathogenic CD4+ T cells that mediate autoimmune-like cGVHD.

Sun Jul 01 00:00:00 EDT 2012

Impact of enzymatic tissue disintegration on the level of surface molecule expression and immune cell function.

European journal of microbiology & immunology

Autengruber A,Gereke M,Hansen G,Hennig C,Bruder D

12-1341 was used in Flow cytometry/Cell sorting to indicate that enzymatic tissue disintegration can have profound effects on the expression of a variety of cell-surface molecules.

Fri Jun 01 00:00:00 EDT 2012

TNF optimally activatives regulatory T cells by inducing TNF receptor superfamily members TNFR2, 4-1BB and OX40.

European journal of immunology

Hamano R,Huang J,Yoshimura T,Oppenheim JJ,Chen X

12-1341 was used in Flow cytometry/Cell sorting to investigate pleiotropy in TNF inflammatory effects, showing that it activates Tregs by inducing TNFR2, 4-1BB and OX40.

Fri Jul 01 00:00:00 EDT 2011

GM-CSF-induced, bone-marrow-derived dendritic cells can expand natural Tregs and induce adaptive Tregs by different mechanisms.

Journal of leukocyte biology

Bhattacharya P,Gopisetty A,Ganesh BB,Sheng JR,Prabhakar BS

12-1341 was used in Flow cytometry experiments to suggest that the BMDCs differentiated by GM-CSF can expand nTregs and induce adaptive Tregs through different mechanisms.

Tue Feb 01 00:00:00 EST 2011

Adenovirus vector-mediated in vivo gene transfer of OX40 ligand to tumor cells enhances antitumor immunity of tumor-bearing hosts.

Cancer research

Andarini S,Kikuchi T,Nukiwa M,Pradono P,Suzuki T,Ohkouchi S,Inoue A,Maemondo M,Ishii N,Saijo Y,Sugamura K,Nukiwa T

Sat May 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: CD134; OX40 antigen; OX40L receptor; tax-transcriptionally activated glycoprotein 1; Tumor necrosis factor receptor superfamily member 4

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Gene Aliases: ACT35; CD134; Ly-70; Ox40; Tnfrsf4; Txgp1; TXGP1L

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UniProt ID: (Mouse) P47741

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Entrez Gene ID: (Mouse) 22163

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Function(s)

tumor necrosis factor-activated receptor activity protein binding transmembrane signal receptor

Process(es)

inflammatory response cellular defense response programmed cell death positive regulation of B cell proliferation tumor necrosis factor-mediated signaling pathway T cell proliferation regulation of apoptotic process negative regulation of sequence-specific DNA binding transcription factor activity regulation of protein kinase activity negative regulation of transcription, DNA-templated negative regulation of cytokine secretion positive regulation of immunoglobulin secretion negative regulation of activation-induced cell death of T cells negative regulation of extrinsic apoptotic signaling pathway

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CD134 (OX40) Monoclonal Antibody (OX-86), PE, eBioscience™

CD134 (OX40) Antibody (12-1341-82) in Flow

Product Details

12-1341-82

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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