Invitrogen

CD133 Polyclonal Antibody

CD133 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

CD133 Antibody (PA5-38014) in ICC/IF

Immunocytochemistry of PROM1 in Jurkat cells with CD133 Polyclonal Antibody (Product # PA5-38014) at 2.5 µg/mL.

Product Image Gallery

Product Details

PA5-38014

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

- -

Immunocytochemistry (ICC/IF)

2.5 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

18 amino acid peptide near the carboxy terminus of human PROM1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2554618

Product Specific Information

A recommended positive control is Jurkat cell lysate.

Multiple isoforms of PROM1 are known to exist.

Target Information

The CD133 (AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. CD133 is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. CD133 has been shown to be expressed in cancers, including some leukemias and brain tumors, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of the CD133 gene is also associated with several types of cancer. CD133 is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner, and multiple transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exosome-mediated inhibition of microRNA-449a promotes the amplification of mouse retinal progenitor cells and enhances their transplantation in retinal degeneration mouse models.

Molecular therapy. Nucleic acids

Guo CJ,Cao XL,Zhang YF,Yue KY,Han J,Yan H,Han H,Zheng MH

PA5-38014 was used in Western Blotting to show that exosome-miR-449a inhibitor can effectively promote the expansion of RPCs in vitro and enhance transplanted RPCs survival in vivo, which might provide a novel intervention strategy for retinal degenerations in the future.

Tue Mar 14 00:00:00 EDT 2023

Organ-specific extracellular matrix directs trans-differentiation of mesenchymal stem cells and formation of salivary gland-like organoids in vivo.

Stem cell research & therapy

Tran ON,Wang H,Li S,Malakhov A,Sun Y,Abdul Azees PA,Gonzalez AO,Cao B,Marinkovic M,Singh BB,Dean DD,Yeh CK,Chen XD

PA5-38014 was used in Flow cytometry/Cell sorting to test the hypothesis that multipotent bone marrow-derived mesenchymal stem cells (BM-MSCs) in a rat model are capable of trans-differentiating to the salivary gland epithelial cell lineage when induced by a native SG-specific extracellular matrix (SG-ECM) and thus may be a viable substitute for repairing damaged SGs.

Fri Jul 15 00:00:00 EDT 2022

Alkaloids of fascaplysin are effective conventional chemotherapeutic drugs, inhibiting the proliferation of C6 glioma cells and causing their death in vitro.

Oncology letters

Bryukhovetskiy I,Lyakhova I,Mischenko P,Milkina E,Zaitsev S,Khotimchenko Y,Bryukhovetskiy A,Polevshchikov A,Kudryavtsev I,Khotimchenko M,Zhidkov M

PA538014 was used in immunocytochemistry to compare the efficiency of fascaplysin with other drugs used to treat glial tumors

Wed Feb 01 00:00:00 EST 2017

miR-200b inhibits CD133⁺ glioma cells by targeting the AKT pathway.

Oncology letters

Liu A,Yu Q,Peng Z,Huang Y,Diao S,Cheng J,Wang W,Hong M

PA5-38014 was used in Magnetic cell separation to explore the fundamental role of miR-200b and CD133 in glioma stem cell behavior.

Thu Jun 01 00:00:00 EDT 2017

Blockade of IDO-kynurenine-AhR metabolic circuitry abrogates IFN-γ-induced immunologic dormancy of tumor-repopulating cells.

Nature communications

Liu Y,Liang X,Yin X,Lv J,Tang K,Ma J,Ji T,Zhang H,Dong W,Jin X,Chen D,Li Y,Zhang S,Xie HQ,Zhao B,Zhao T,Lu J,Hu ZW,Cao X,Qin FX,Huang B

Published figure using CD133 polyclonal antibody (Product # PA5-38014) in Flow Cytometry

Wed May 10 00:00:00 EDT 2017

LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4.

Journal of experimental & clinical cancer research : CR

Han S,Wang C,Qin X,Xia J,Wu A

PA5-38014 was used in Western Blotting to examine the ability of lipopolysaccharise to affect glioma and glioma stem-like cells in vitro and to induce antitumour immunity in vivo and the role of TLR4 in these processes.

Thu Jun 22 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Antigen AC133; Antigen AC133 homolog; antigen CD133; CD133; fudenine; hematopoietic stem cell antigen; hProminin; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; Mac3; Prominin-1; prominin-like 1; Prominin-like protein 1; Prominin1

View more View less

Gene Aliases: 4932416E19Rik; AC133; CD133; CORD12; MCDR2; MSTP061; Prom; Prom-1; PROM1; PROML1; RP41; STGD4

View more View less

UniProt ID: (Human) Q6SV49, (Mouse) O54990

View more View less

Entrez Gene ID: (Human) 8842, (Mouse) 19126, (Rat) 60357

View more View less

Function(s)

protein binding actinin binding cadherin binding molecular_function

Process(es)

retina layer formation photoreceptor cell maintenance retina morphogenesis in camera-type eye camera-type eye photoreceptor cell differentiation glomerular visceral epithelial cell differentiation glomerular parietal epithelial cell differentiation positive regulation of nephron tubule epithelial cell differentiation biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD133 Polyclonal Antibody

CD133 Antibody (PA5-38014) in ICC/IF

Product Details

PA5-38014

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!