Invitrogen

CD11b Monoclonal Antibody (ICRF44), eBioscience™

FIGURE: 1 / 36

CD11b Antibody (14-0118-82) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD11b PE. Autofluorescence is indicated by open histogram. Cells in the monocyte population were used for analysis.

CD11b Antibody in Immunocytochemistry (ICC/IF)

CD11b Antibody in Immunohistochemistry (IHC)

CD11b Antibody in Western Blot, Flow Cytometry (WB, Flow)

Product Image Gallery

Product Details

14-0118-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

1 µg/test

View 44 publications 44 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Neutralization (Neu)

Assay-Dependent

Functional Assay (Functional)

Assay-Dependent

Product Specifications

Species Reactivity

Baboon, Chimpanzee, Cynomolgus monkey, Human, Rhesus monkey

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ICRF44

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467120

Product Specific Information

Description: The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.

Applications Reported: The ICRF44 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining on formaldehyde fixed tissue. ICRF44 has also been reported in inhibition of aggregation of PMNs.

Applications Tested: The ICRF44 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ELMO1 Deficiency Reduces Neutrophil Chemotaxis in Murine Peritonitis.

International journal of molecular sciences

Yu S,Geng X,Liu H,Zhang Y,Cao X,Li B,Yan J

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Sun Apr 30 00:00:00 EDT 2023

Regulation of synaptic connectivity in schizophrenia spectrum by mutual neuron-microglia interaction.

Communications biology

Breitmeyer R,Vogel S,Heider J,Hartmann SM,Wüst R,Keller AL,Binner A,Fitzgerald JC,Fallgatter AJ,Volkmer H

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Sat Apr 29 00:00:00 EDT 2023

Regulation of synaptic connectivity in schizophrenia spectrum by mutual neuron-microglia interaction.

Communications biology

Breitmeyer R,Vogel S,Heider J,Hartmann SM,Wüst R,Keller AL,Binner A,Fitzgerald JC,Fallgatter AJ,Volkmer H

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Sat Apr 29 00:00:00 EDT 2023

LAL deficiency induced myeloid-derived suppressor cells as targets and biomarkers for lung cancer.

Journal for immunotherapy of cancer

Zhao T,Liu S,Hanna NH,Jalal S,Ding X,Wan J,Yan C,Du H

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Investigating Therapeutic Effects of Indole Derivatives Targeting Inflammation and Oxidative Stress in Neurotoxin-Induced Cell and Mouse Models of Parkinson's Disease.

International journal of molecular sciences

Chiu YJ,Lin CH,Lin CY,Yang PN,Lo YS,Chen YC,Chen CM,Wu YR,Yao CF,Chang KH,Lee-Chen GJ

14-0118-82 was used in Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Neutralization, Immunocytochemistry to find the potential of indole derivative NC009-1 against neuroinflammation, oxidative stress, and neurodegeneration for PD.

Mon Jan 30 00:00:00 EST 2023

SARS-CoV-2 Delta (B.1.617.2) variant replicates and induces syncytia formation in human induced pluripotent stem cell-derived macrophages.

PeerJ

Thaweerattanasinp T,Wanitchang A,Saenboonrueng J,Srisutthisamphan K,Wanasen N,Sungsuwan S,Jongkaewwattana A,Chailangkarn T

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2023

Post-amputation reactive oxygen species production is necessary for axolotls limb regeneration.

Frontiers in cell and developmental biology

Carbonell-M B,Zapata Cardona J,Delgado JP

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Immunohistochemistry

Tue Sep 13 00:00:00 EDT 2022

Methylation of SPRED1: A New Target in Acute Myeloid Leukemia.

Frontiers in oncology

Su N,Wang Y,Lu X,Xu W,Wang H,Mo W,Pang H,Tang R,Li S,Yan X,Li Y,Zhang R

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Sat Apr 02 00:00:00 EDT 2022

Prognostic Lnc-S100B-2 Affects Cell Apoptosis and Microenvironment of Colorectal Cancer through MLLT10 Signaling.

Journal of oncology

Yi J,Peng F,Zhao J,Gong X

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Tue Feb 08 00:00:00 EST 2022

miR-204-containing exosomes ameliorate GVHD-associated dry eye disease.

Science advances

Zhou T,He C,Lai P,Yang Z,Liu Y,Xu H,Lin X,Ni B,Ju R,Yi W,Liang L,Pei D,Egwuagu CE,Liu X

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Fri Jan 14 00:00:00 EST 2022

The Transcription of ZIP9 Is Associated With the Macrophage Polarization and the Pathogenesis of Hepatocellular Carcinoma.

Frontiers in immunology

Gou Y,Yang D,Tian T,Zhu X,Zhang R,Ren J,Tu D,Luo Y,Miao Y,Zhao H,Wang Y,Wei B

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Fri Apr 08 00:00:00 EDT 2022

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Different Induction of PD-L1 (CD274) and PD-1 (CD279) Expression in THP-1-Differentiated Types 1 and 2 Macrophages.

Journal of inflammation research

Lai CY,Tseng PC,Chen CL,Satria RD,Wang YT,Lin CF

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Wed Apr 27 00:00:00 EDT 2022

G-MDSCs promote aging-related cardiac fibrosis by activating myofibroblasts and preventing senescence.

Cell death & disease

Sun SN,Ni SH,Li Y,Liu X,Deng JP,Chen ZX,Li H,Feng WJ,Huang YS,Li DN,Xian SX,Yang ZQ,Wang LJ,Lu L

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.

International journal of inflammation

Genkel V,Dolgushin I,Baturina I,Savochkina A,Kuznetsova A,Pykhova L,Shaposhnik I

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Sun Apr 24 00:00:00 EDT 2022

Persistent NF-κB activation in muscle stem cells induces proliferation-independent telomere shortening.

Cell reports

Tichy ED,Ma N,Sidibe D,Loro E,Kocan J,Chen DZ,Khurana TS,Hasty P,Mourkioti F

14-0118-82 was used in Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Neutralization to investigate NF-kB signalling on muscle stem cells.

Tue May 11 00:00:00 EDT 2021

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

Molecular cell

Psaila B,Wang G,Rodriguez-Meira A,Li R,Heuston EF,Murphy L,Yee D,Hitchcock IS,Sousos N,O'Sullivan J,Anderson S,Senis YA,Weinberg OK,Calicchio ML,Iskander D,Royston D,Milojkovic D,Roberts I,Bodine DM,Thongjuea S,Mead AJ

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Thu May 07 00:00:00 EDT 2020

The long non-coding RNA Cancer Susceptibility 15 (CASC15) is induced by isocitrate dehydrogenase (IDH) mutations and maintains an immature phenotype in adult acute myeloid leukemia.

Haematologica

Grasedieck S,Ruess C,Krowiorz K,Lux S,Pochert N,Schwarzer A,Klusmann JH,Jongen-Lavrencic M,Herold T,Bullinger L,Pollack JR,Rouhi A,Kuchenbauer F

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Galectin-9 promotes a suppressive microenvironment in human cancer by enhancing STING degradation.

Oncogenesis

Zhang CX,Huang DJ,Baloche V,Zhang L,Xu JX,Li BW,Zhao XR,He J,Mai HQ,Chen QY,Zhang XS,Busson P,Cui J,Li J

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Connecting METTL3 and intratumoural CD33⁺ MDSCs in predicting clinical outcome in cervical cancer.

Journal of translational medicine

Ni HH,Zhang L,Huang H,Dai SQ,Li J

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Thu Oct 15 00:00:00 EDT 2020

Phase I Study of Ficlatuzumab and Cetuximab in Cetuximab-Resistant, Recurrent/Metastatic Head and Neck Cancer.

Cancers

Bauman JE,Ohr J,Gooding WE,Ferris RL,Duvvuri U,Kim S,Johnson JT,Soloff AC,Wallweber G,Winslow J,Gaither-Davis A,Grandis JR,Stabile LP

14-0118-82 was used in Flow Cytometry to establish the recommended Phase II dose (RP2D). Secondary objectives included overall response rate (ORR), progression-free survival (PFS), and overall survival (OS).

Thu Jun 11 00:00:00 EDT 2020

TLR1/2 ligand enhances antitumor efficacy of CTLA-4 blockade by increasing intratumoral Treg depletion.

Proceedings of the National Academy of Sciences of the United States of America

Sharma N,Vacher J,Allison JP

14-0118-82 was used in Flow Cytometry to show that this mechanism could be harnessed to modulate the clinical outcome of anti-CTLA-4 antibodies and possibly other antibody-based immunotherapies.

Tue May 21 00:00:00 EDT 2019

Ubiquitin-specific peptidase 3 induces TPA-mediated leukemia cell differentiation via regulating H2AK119ub.

Animal cells and systems

Chae YC,Jung H,Kim JY,Lee DH,Seo SB

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

A rapid in vitro methodology for simultaneous target discovery and antibody generation against functional cell subpopulations.

Scientific reports

Nixon AML,Duque A,Yelle N,McLaughlin M,Davoudi S,Pedley NM,Haynes J,Brown KR,Pan J,Hart T,Gilbert PM,Singh SK,O'Brien CA,Sidhu SS,Moffat J

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Tue Jan 29 00:00:00 EST 2019

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Thu May 09 00:00:00 EDT 2019

Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner.

Cell

Thion MS,Low D,Silvin A,Chen J,Grisel P,Schulte-Schrepping J,Blecher R,Ulas T,Squarzoni P,Hoeffel G,Coulpier F,Siopi E,David FS,Scholz C,Shihui F,Lum J,Amoyo AA,Larbi A,Poidinger M,Buttgereit A,Lledo PM,Greter M,Chan JKY,Amit I,Beyer M,Schultze JL,Schlitzer A,Pettersson S,Ginhoux F,Garel S

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

AURKA Suppresses Leukemic THP-1 Cell Differentiation through Inhibition of the KDM6B Pathway.

Molecules and cells

Park JW,Cho H,Oh H,Kim JY,Seo SB

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Thu May 31 00:00:00 EDT 2018

n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.

Molecular medicine reports

Wu B,Wang L,Jiang L,Dong L,Xu F,Lu Y,Jin J,Wang Z,Liang G,Shan X

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.

Oncology letters

Chen X,Wang Y,Wang J,Wen J,Jia X,Wang X,Zhang H

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2018

Deacetylase activity-independent transcriptional activation by HDAC2 during TPA-induced HL-60 cell differentiation.

PloS one

Jung H,Kim JY,Kim KB,Chae YC,Hahn Y,Kim JW,Seo SB

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Thu Feb 07 00:00:00 EST 2019

Microglia innately develop within cerebral organoids.

Nature communications

Ormel PR,Vieira de Sá R,van Bodegraven EJ,Karst H,Harschnitz O,Sneeboer MAM,Johansen LE,van Dijk RE,Scheefhals N,Berdenis van Berlekom A,Ribes Martínez E,Kling S,MacGillavry HD,van den Berg LH,Kahn RS,Hol EM,de Witte LD,Pasterkamp RJ

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Tue Oct 09 00:00:00 EDT 2018

Macrophages are exploited from an innate wound healing response to facilitate cancer metastasis.

Nature communications

Muliaditan T,Caron J,Okesola M,Opzoomer JW,Kosti P,Georgouli M,Gordon P,Lall S,Kuzeva DM,Pedro L,Shields JD,Gillett CE,Diebold SS,Sanz-Moreno V,Ng T,Hoste E,Arnold JN

14-0118 was used in Immunohistochemistry-immunofluorescence to show that tumours can exploit this response to facilitate transendothelial migration and metastatic spread of the disease, which can be pharmacologically targeted using a clinically relevant HO-1 inhibitor.

Fri Jul 27 00:00:00 EDT 2018

The prognostic significance of CD11b⁺CX3CR1⁺ monocytes in patients with newly diagnosed diffuse large B-cell lymphoma.

Oncotarget

Yhim HY,Kim JA,Ko SH,Park Y,Yim E,Kim HS,Kwak JY

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Fri Nov 03 00:00:00 EDT 2017

CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity.

Nature communications

Jacoby E,Nguyen SM,Fountaine TJ,Welp K,Gryder B,Qin H,Yang Y,Chien CD,Seif AE,Lei H,Song YK,Khan J,Lee DW,Mackall CL,Gardner RA,Jensen MC,Shern JF,Fry TJ

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Wed Jul 27 00:00:00 EDT 2016

The prognostic effects of tumor infiltrating regulatory T cells and myeloid derived suppressor cells assessed by multicolor flow cytometry in gastric cancer patients.

Oncotarget

Choi HS,Ha SY,Kim HM,Ahn SM,Kang MS,Kim KM,Choi MG,Lee JH,Sohn TS,Bae JM,Kim S,Kang ES

14-0118 was used in Flow cytometry/Cell sorting to demonstrate that in gastric cancer, a high frequency of Tregs among tumour-infiltrating CD4+ T cells is favourable, but an increased proportion of MDSCs is an adverse independent prognostic factor.

Tue Feb 16 00:00:00 EST 2016

Metabolic regulation of hepatitis B immunopathology by myeloid-derived suppressor cells.

Nature medicine

Pallett LJ,Gill US,Quaglia A,Sinclair LV,Jover-Cobos M,Schurich A,Singh KP,Thomas N,Das A,Chen A,Fusai G,Bertoletti A,Cantrell DA,Kennedy PT,Davies NA,Haniffa M,Maini MK

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

STK4 regulates TLR pathways and protects against chronic inflammation-related hepatocellular carcinoma.

The Journal of clinical investigation

Li W,Xiao J,Zhou X,Xu M,Hu C,Xu X,Lu Y,Liu C,Xue S,Nie L,Zhang H,Li Z,Zhang Y,Ji F,Hui L,Tao W,Wei B,Wang H

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Mon Nov 02 00:00:00 EST 2015

Induction of autophagy is a key component of all-trans-retinoic acid-induced differentiation in leukemia cells and a potential target for pharmacologic modulation.

Experimental hematology

Orfali N,O'Donovan TR,Nyhan MJ,Britschgi A,Tschan MP,Cahill MR,Mongan NP,Gudas LJ,McKenna SL

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Induction of autophagy is a key component of all-trans-retinoic acid-induced differentiation in leukemia cells and a potential target for pharmacologic modulation.

Experimental hematology

Orfali N,O'Donovan TR,Nyhan MJ,Britschgi A,Tschan MP,Cahill MR,Mongan NP,Gudas LJ,McKenna SL

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Activation status of human microglia is dependent on lesion formation stage and remyelination in multiple sclerosis.

Journal of neuropathology and experimental neurology

Peferoen LA,Vogel DY,Ummenthum K,Breur M,Heijnen PD,Gerritsen WH,Peferoen-Baert RM,van der Valk P,Dijkstra CD,Amor S

14-0118 was used in Flow cytometry/Cell sorting to define the differential activation states of microglia during lesion development in multiple sclerosis-affected CNS tissues.

Thu Jan 01 00:00:00 EST 2015

dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.

Nature communications

Lim S,Kim WJ,Kim YH,Lee S,Koo JH,Lee JA,Yoon H,Kim DH,Park HJ,Kim HM,Lee HG,Yun Kim J,Lee JU,Hun Shin J,Kyun Kim L,Doh J,Kim H,Lee SK,Bothwell ALM,Suh M,Choi JM

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Tue Sep 15 00:00:00 EDT 2015

Identification of Reprogrammed Myeloid Cell Transcriptomes in NSCLC.

PloS one

Durrans A,Gao D,Gupta R,Fischer KR,Choi H,El Rayes T,Ryu S,Nasar A,Spinelli CF,Andrews W,Elemento O,Nolan D,Stiles B,Rafii S,Narula N,Davuluri R,Altorki NK,Mittal V

14-0118 was used in Immunofluorescence to demonstrate that analysis of homogeneous stromal populations isolated from fresh clinical specimens can detect important stromal genes.

Fri Mar 25 00:00:00 EDT 2016

Microbe-specific unconventional T cells induce human neutrophil differentiation into antigen cross-presenting cells.

Journal of immunology (Baltimore, Md. : 1950)

Davey MS,Morgan MP,Liuzzi AR,Tyler CJ,Khan MWA,Szakmany T,Hall JE,Moser B,Eberl M

14-0118 was used in Immunofluorescence to investigate how unconventional T cells represent key controllers of neutrophil-driven innate and adaptive responses to a broad range of pathogens.

Wed Oct 01 00:00:00 EDT 2014

DNGR-1 is a specific and universal marker of mouse and human Batf3-dependent dendritic cells in lymphoid and nonlymphoid tissues.

Blood

Poulin LF,Reyal Y,Uronen-Hansson H,Schraml BU,Sancho D,Murphy KM,Håkansson UK,Moita LF,Agace WW,Bonnet D,Reis e Sousa C

14-0118 was used in Flow cytometry/Cell sorting to investigate the presence of unique markers in human dendritic cells, showing that DNGR-1 is a specific marker of mouse and human dendritic cells in lymphoid and nonlymphoid tissues.

Thu Jun 21 00:00:00 EDT 2012

Resolvin D1 limits polymorphonuclear leukocyte recruitment to inflammatory loci: receptor-dependent actions.

Arteriosclerosis, thrombosis, and vascular biology

Norling LV,Dalli J,Flower RJ,Serhan CN,Perretti M

14-0118 was used in Flow cytometry/Cell sorting to study the role of Resolvin D1 in limiting neutrophil recruitment during acute inflammation.

Wed Aug 01 00:00:00 EDT 2012

Heparin-binding epidermal growth factor-like growth factor (HB-EGF) preserves gut barrier function by blocking neutrophil-endothelial cell adhesion after hemorrhagic shock and resuscitation in mice.

Surgery

Zhang HY,James I,Chen CL,Besner GE

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Heparin-binding epidermal growth factor-like growth factor (HB-EGF) preserves gut barrier function by blocking neutrophil-endothelial cell adhesion after hemorrhagic shock and resuscitation in mice.

Surgery

Zhang HY,James I,Chen CL,Besner GE

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Human neutrophil clearance of bacterial pathogens triggers anti-microbial γδ T cell responses in early infection.

PLoS pathogens

Davey MS,Lin CY,Roberts GW,Heuston S,Brown AC,Chess JA,Toleman MA,Gahan CG,Hill C,Parish T,Williams JD,Davies SJ,Johnson DW,Topley N,Moser B,Eberl M

14-0118 was used in Flow cytometry/Cell sorting to demonstrate that Vγ9/Vδ2 T cells provide potent survival signals resulting in neutrophil activation and the release of the neutrophil chemoattractant CXCL8.

Sun May 01 00:00:00 EDT 2011

Endogenous PMN sialidase activity exposes activation epitope on CD11b/CD18 which enhances its binding interaction with ICAM-1.

Journal of leukocyte biology

Feng C,Zhang L,Almulki L,Faez S,Whitford M,Hafezi-Moghadam A,Cross AS

14-0118 was used in Immunoprecipitation to investigate how sialylation of adhesion molecules expressed on endothelial cells and polymorphonuclear cells affects the adhesion between these cells.

Mon Aug 01 00:00:00 EDT 2011

Generation of bivalent chromatin domains during cell fate decisions.

Epigenetics & chromatin

De Gobbi M,Garrick D,Lynch M,Vernimmen D,Hughes JR,Goardon N,Luc S,Lower KM,Sloane-Stanley JA,Pina C,Soneji S,Renella R,Enver T,Taylor S,Jacobsen SE,Vyas P,Gibbons RJ,Higgs DR

14-0118 was used in Flow cytometry/Cell sorting to investigate the patterns and levels of histone modifications at bivalent genes, showing the generation of bivalent chromatin domains during cell fate decisions.

Mon Jun 06 00:00:00 EDT 2011

Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells.

The Journal of experimental medicine

Poulin LF,Salio M,Griessinger E,Anjos-Afonso F,Craciun L,Chen JL,Keller AM,Joffre O,Zelenay S,Nye E,Le Moine A,Faure F,Donckier V,Sancho D,Cerundolo V,Bonnet D,Reis e Sousa C

14-0118 was used in Flow cytometry/Cell sorting to investigate a population of human DCs that expresses DNGR-1 and high levels of BDCA3.

Mon Jun 07 00:00:00 EDT 2010

Normal human monocytes exposed to glioma cells acquire myeloid-derived suppressor cell-like properties.

Neuro-oncology

Rodrigues JC,Gonzalez GC,Zhang L,Ibrahim G,Kelly JJ,Gustafson MP,Lin Y,Dietz AB,Forsyth PA,Yong VW,Parney IF

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2010

Normal human monocytes exposed to glioma cells acquire myeloid-derived suppressor cell-like properties.

Neuro-oncology

Rodrigues JC,Gonzalez GC,Zhang L,Ibrahim G,Kelly JJ,Gustafson MP,Lin Y,Dietz AB,Forsyth PA,Yong VW,Parney IF

Published figure using CD11b monoclonal antibody (Product # 14-0118-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2010

IL-15 trans-presentation promotes human NK cell development and differentiation in vivo.

The Journal of experimental medicine

Huntington ND,Legrand N,Alves NL,Jaron B,Weijer K,Plet A,Corcuff E,Mortier E,Jacques Y,Spits H,Di Santo JP

14-0118-82 was used in Flow Cytometry to assess the role of interleukin-15 in human natural killer cell development.

Fri Jan 16 00:00:00 EST 2009

Membrane mobility of beta2 integrins and rolling associated adhesion molecules in resting neutrophils.

Biophysical journal

Gaborski TR,Clark A,Waugh RE,McGrath JL

14-0118 was used in Immunofluorescence to present an analytical model to measure the mobility of the four key receptors involved in the neutrophil adhesion cascade.

Sat Nov 15 00:00:00 EST 2008

Bioinformatics

Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; integrin alpha M; Integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); Leukocyte adhesion receptor MO1; Mac-1 alpha (Mac1A); MAC-1 alpha subunit; macrophage antigen alpha polypeptide; MGC117044; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; unnamed protein product

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Gene Aliases: CD11B; CR3A; ITGAM; MAC-1; MAC1A; MO1A; SLEB6

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UniProt ID: (Human) P11215

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Entrez Gene ID: (Human) 3684, (Rhesus monkey) 714832, (Cynomolgus monkey) 101866035, (Chimpanzee) 454069

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Function(s)

glycoprotein binding protein binding metal ion binding integrin

Process(es)

cell adhesion integrin-mediated signaling pathway ectodermal cell differentiation extracellular matrix organization toll-like receptor 4 signaling pathway leukocyte migration

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CD11b Monoclonal Antibody (ICRF44), eBioscience™

CD11b Antibody (14-0118-82) in Flow

Product Details

14-0118-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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