Invitrogen

CD117 (c-Kit) Monoclonal Antibody (ACK2), PE, eBioscience™

CD117 (c-Kit) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 20

CD117 (c-Kit) Antibody (12-1172-82) in Flow

C57BL/6 mouse bone marrow cells were stained with Mouse Hematopoietic Lineage Antibody Cocktail, eFluor 450 (Product # 88-7772-72), Sca-1 Monoclonal Antibody, FITC (Product # 11-5981-82), and 0.06 µg of Rat IgG2b kappa Isotype Control, PE (Product # 12-4031-83) (left) or 0.06 µg of CD117 (c-Kit) Monoclonal Antibody, PE (right). Cells in the Lineage low/negative gate were used for analysis.

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Product Details

12-1172-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 27 publications 27 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ACK2

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465816

Product Specific Information

Description: The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Applications Reported: The ACK2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The ACK2 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Engineered bone marrow as a clinically relevant ex vivo model for primary bone cancer research and drug screening.

Proceedings of the National Academy of Sciences of the United States of America

Griffin KH,Thorpe SW,Sebastian A,Hum NR,Coonan TP,Sagheb IS,Loots GG,Randall RL,Leach JK

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 12-1172-82) in Flow Cytometry

Tue Sep 26 00:00:00 EDT 2023

Landscape of mast cell populations across organs in mice and humans.

The Journal of experimental medicine

Tauber M,Basso L,Martin J,Bostan L,Pinto MM,Thierry GR,Houmadi R,Serhan N,Loste A,Blériot C,Kamphuis JBJ,Grujic M,Kjellén L,Pejler G,Paul C,Dong X,Galli SJ,Reber LL,Ginhoux F,Bajenoff M,Gentek R,Gaudenzio N

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 12-1172-82) in Flow Cytometry

Mon Oct 02 00:00:00 EDT 2023

The AML-associated K313 mutation enhances C/EBPα activity by leading to C/EBPα overexpression.

Cell death & disease

Gentle IE,Moelter I,Badr MT,Döhner K,Lübbert M,Häcker G

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 12-1172-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Mutations in the iron-sulfur cluster biogenesis protein HSCB cause congenital sideroblastic anemia.

The Journal of clinical investigation

Crispin A,Guo C,Chen C,Campagna DR,Schmidt PJ,Lichtenstein D,Cao C,Sendamarai AK,Hildick-Smith GJ,Huston NC,Boudreaux J,Bottomley SS,Heeney MM,Paw BH,Fleming MD,Ducamp S

12-1172 was used in Flow cytometry/Cell sorting to find that HSCB expression was decreased in patient-derived fibroblasts and K562 erythroleukemia cells engineered to have the patient-specific promoter variant.

Thu Oct 01 00:00:00 EDT 2020

The lifespan quantitative trait locus gene Securin controls hematopoietic progenitor cell function.

Haematologica

Brown A,Schuetz D,Han Y,Daria D,Nattamai KJ,Eiwen K,Sakk V,Pospiech J,Saller T,van Zant G,Wagner W,Geiger H

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 12-1172-82) in Flow Cytometry

Tue Apr 27 00:00:00 EDT 2021

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 12-1172-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Loss of FGFR3 Delays Acute Myeloid Leukemogenesis by Programming Weakly Pathogenic CD117-Positive Leukemia Stem-Like Cells.

Frontiers in pharmacology

Guo C,Ran Q,Sun C,Zhou T,Yang X,Zhang J,Pang S,Xiao Y

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 12-1172-82) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2021

GM-CSF Calibrates Macrophage Defense and Wound Healing Programs during Intestinal Infection and Inflammation.

Cell reports

Castro-Dopico T,Fleming A,Dennison TW,Ferdinand JR,Harcourt K,Stewart BJ,Cader Z,Tuong ZK,Jing C,Lok LSC,Mathews RJ,Portet A,Kaser A,Clare S,Clatworthy MR

12-1172 was used in Flow cytometry/Cell sorting to identify granulocyte-macrophage colony stimulating factor (GM-CSF) as a critical regulator of intestinal macrophage activation in patients with IBD and mice with dextran sodium sulfate (DSS)-induced colitis.

Tue Jul 07 00:00:00 EDT 2020

Interleukin-17-Producing γδ T Cells Originate from SOX13⁺ Progenitors that Are Independent of γδTCR Signaling.

Immunity

Spidale NA,Sylvia K,Narayan K,Miu B,Frascoli M,Melichar HJ,Zhihao W,Kisielow J,Palin A,Serwold T,Love P,Kobayashi M,Yoshimoto M,Jain N,Kang J

12-1172-82 was used in Flow Cytometry to generate reporter mice for the Tγδ17 lineage-defining transcription factor SOX13 and identify fetal-origin, intrathymic Sox13+ progenitors.

Tue Nov 20 00:00:00 EST 2018

Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply.

Nature communications

Liu C,Han T,Stachura DL,Wang H,Vaisman BL,Kim J,Klemke RL,Remaley AT,Rana TM,Traver D,Miller YI

12-1172 was used in Flow cytometry/Cell sorting to demonstrate the requirement for lipoprotein lipase activity in haematopoietic stem progenitor cell maintenance.

Tue Apr 03 00:00:00 EDT 2018

GATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis.

The Journal of clinical investigation

Géraud C,Koch PS,Zierow J,Klapproth K,Busch K,Olsavszky V,Leibing T,Demory A,Ulbrich F,Diett M,Singh S,Sticht C,Breitkopf-Heinlein K,Richter K,Karppinen SM,Pihlajaniemi T,Arnold B,Rodewald HR,Augustin HG,Schledzewski K,Goerdt S

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 12-1172-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Low Testosterone Alters the Activity of Mouse Prostate Stem Cells.

The Prostate

Zhou Y,Copeland B,Otto-Duessel M,He M,Markel S,Synold TW,Jones JO

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 12-1172-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

A Functional Bioluminescent Zebrafish Screen for Enhancing Hematopoietic Cell Homing.

Stem cell reports

Astuti Y,Kramer AC,Blake AL,Blazar BR,Tolar J,Taisto ME,Lund TC

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 12-1172-82) in Flow Cytometry

Tue Jan 10 00:00:00 EST 2017

Mutant U2AF1-expressing cells are sensitive to pharmacological modulation of the spliceosome.

Nature communications

Shirai CL,White BS,Tripathi M,Tapia R,Ley JN,Ndonwi M,Kim S,Shao J,Carver A,Saez B,Fulton RS,Fronick C,O'Laughlin M,Lagisetti C,Webb TR,Graubert TA,Walter MJ

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 12-1172-82) in Flow Cytometry

Mon Jan 09 00:00:00 EST 2017

Core Circadian Clock Genes Regulate Leukemia Stem Cells in AML.

Cell

Puram RV,Kowalczyk MS,de Boer CG,Schneider RK,Miller PG,McConkey M,Tothova Z,Tejero H,Heckl D,Järås M,Chen MC,Li H,Tamayo A,Cowley GS,Rozenblatt-Rosen O,Al-Shahrour F,Regev A,Ebert BL

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 12-1172-82) in Flow Cytometry

Thu Apr 07 00:00:00 EDT 2016

Wnt-β-Catenin Signaling Promotes the Maturation of Mast Cells.

BioMed research international

Yamaguchi T,Nishijima M,Tashiro K,Kawabata K

12-1172 was used in Flow cytometry/Cell sorting to show that Wnt5a could promote the maturation of mast cells via the canonical Wnt signaling pathway.

Thu Jan 19 00:00:00 EST 2017

Regulation of B-cell development and tolerance by different members of the miR-17∼92 family microRNAs.

Nature communications

Lai M,Gonzalez-Martin A,Cooper AB,Oda H,Jin HY,Shepherd J,He L,Zhu J,Nemazee D,Xiao C

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 12-1172-82) in Flow Cytometry

Tue Aug 02 00:00:00 EDT 2016

Retinoid-X-receptors (α/β) in melanocytes modulate innate immune responses and differentially regulate cell survival following UV irradiation.

PLoS genetics

Coleman DJ,Garcia G,Hyter S,Jang HS,Chagani S,Liang X,Larue L,Ganguli-Indra G,Indra AK

12-1172 was used in Flow cytometry/Cell sorting to characterise a novel immunoregulatory role of retinoid-X receptors in melanocytes, identifying them as potential targets for therapy against UV induced melanoma.

Thu May 01 00:00:00 EDT 2014

TRPA1-dependent pruritus in IL-13-induced chronic atopic dermatitis.

Journal of immunology (Baltimore, Md. : 1950)

Oh MH,Oh SY,Lu J,Lou H,Myers AC,Zhu Z,Zheng T

Published figure using CD117 (c-Kit) monoclonal antibody (Product # 12-1172-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2013

Wiskott-Aldrich syndrome protein is required for homeostasis and function of invariant NKT cells.

Journal of immunology (Baltimore, Md. : 1950)

Astrakhan A,Ochs HD,Rawlings DJ

12-1172 was used in Flow cytometry/Cell sorting to investigate the role for WASp within the iNKT lineage.

Mon Jun 15 00:00:00 EDT 2009

Protein kinase C{gamma} mediates regulation of proliferation by the serotonin 5-hydroxytryptamine receptor 2B.

The Journal of biological chemistry

Wouters MM,Roeder JL,Tharayil VS,Stanich JE,Strege PR,Lei S,Bardsley MR,Ordog T,Gibbons SJ,Farrugia G

12-1172 was used in Flow cytometry/Cell sorting to characterise the mitogenic signal transduction cascade activated by the 5-hydroxytryptamine receptor 2B receptor.

Fri Aug 07 00:00:00 EDT 2009

Mast cells are crucial for early inflammation, migration of Langerhans cells, and CTL responses following topical application of TLR7 ligand in mice.

Blood

Heib V,Becker M,Warger T,Rechtsteiner G,Tertilt C,Klein M,Bopp T,Taube C,Schild H,Schmitt E,Stassen M

12-1172 was used in Flow cytometry/Cell sorting to demonstrate the potent versatility of alternatively activated mast cells at the interface of innate and adaptive immunity.

Wed Aug 01 00:00:00 EDT 2007

Selective labeling and isolation of functional classes of interstitial cells of Cajal of human and murine small intestine.

American journal of physiology. Cell physiology

Chen H,Redelman D,Ro S,Ward SM,Ordög T,Sanders KM

12-1172 was used in Flow cytometry/Cell sorting to investigate different functional classes of interstitial cells of Cajal of human and murine small intestine.

Mon Jan 01 00:00:00 EST 2007

Differential gene expression in functional classes of interstitial cells of Cajal in murine small intestine.

Physiological genomics

Chen H,Ordög T,Chen J,Young DL,Bardsley MR,Redelman D,Ward SM,Sanders KM

12-1172 was used in Flow cytometry/Cell sorting to isolate the two functional ICC classes from enzymatically dispersed intestinal muscles by fluorescence-activated cell sorting.

Wed Nov 14 00:00:00 EST 2007

Delayed, asynchronous, and reversible T-lineage specification induced by Notch/Delta signaling.

Genes & development

Taghon TN,David ES,Zúñiga-Pflücker JC,Rothenberg EV

12-1172 was used in Flow cytometry/Cell sorting to study how Notch/Delta signaling is not sufficient for T-lineage specification and commitment, but can be permissive for the maintenance and proliferation of uncommitted progenitors.

Fri Apr 15 00:00:00 EDT 2005

Beta-catenin is dispensable for hematopoiesis and lymphopoiesis.

The Journal of experimental medicine

Cobas M,Wilson A,Ernst B,Mancini SJ,MacDonald HR,Kemler R,Radtke F

12-1172 was used in Flow cytometry/Cell sorting to investigate an essential role for beta-catenin during hematopoiesis and lymphopoiesis.

Mon Jan 19 00:00:00 EST 2004

Involvement of death receptor Fas in germ cell degeneration in gonads of Kit-deficient Wv/Wv mutant mice.

Cell death and differentiation

Sakata S,Sakamaki K,Watanabe K,Nakamura N,Toyokuni S,Nishimune Y,Mori C,Yonehara S

12-1172-82 was used in Flow Cytometry to indicate that Fas-mediated apoptosis is involved in germ cell degeneration accompanied by defects in Kit-mediated signals, and Kit signaling negatively regulates Fas-mediated apoptosis in vivo.

Sun Jun 01 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: belly-spot; c-kit proto-oncogene protein; CD117; ckit; dominant spotting; Dominant white spotting; Mast/stem cell growth factor receptor Kit; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; spotted sterile male; Steel Factor Receptor; Tyrosine-protein kinase Kit

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Gene Aliases: Bs; c-KIT; CD117; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; Kit; SCO1; SCO5; Sl; SOW3; Ssm; Tr-kit; W

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UniProt ID: (Mouse) P05532

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Entrez Gene ID: (Mouse) 16590

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Function(s)

nucleotide binding protease binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity stem cell factor receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups cytokine binding protein homodimerization activity metal ion binding transmembrane signal receptor

Process(es)

Performance Guarantee

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CD117 (c-Kit) Monoclonal Antibody (ACK2), PE, eBioscience™

CD117 (c-Kit) Antibody (12-1172-82) in Flow

Product Details

12-1172-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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