Invitrogen

CD105 (Endoglin) Monoclonal Antibody (SN6), Super Bright™ 645, eBioscience™

CD105 (Endoglin) Antibody in Flow Cytometry (Flow)

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CD105 (Endoglin) Antibody (64-1057-42) in Flow

Staining of U937 cells with Mouse IgG1 K Isotype Control Super Bright 645 (Product # 64-4714-82) (blue histogram) or CD105 (Endoglin) Monoclonal Antibody, Super Bright 645 (purple histogram). Total viable cells were used for analysis.

CD105 (Endoglin) Antibody in Immunocytochemistry (ICC/IF)

CD105 (Endoglin) Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

64-1057-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 30 publications 30 publications

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 645, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SN6

Conjugate

Super Bright™ 645 Super Bright™ 645 Super Bright™ 645

Excitation/Emission Max

414/645 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2717099

Product Specific Information

Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin. CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.

Applications Reported: The SN6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SN6 antibody has been pre-titrated and tested by flow cytometric analysis of U937 cell line. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Enhanced chondrogenic differentiation of iPS cell-derived mesenchymal stem/stromal cells via neural crest cell induction for hyaline cartilage repair.

Frontiers in cell and developmental biology

Zujur D,Al-Akashi Z,Nakamura A,Zhao C,Takahashi K,Aritomi S,Theoputra W,Kamiya D,Nakayama K,Ikeya M

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Sun May 28 00:00:00 EDT 2023

Exercise combined with administration of adipose-derived stem cells ameliorates neuropathic pain after spinal cord injury.

Neural regeneration research

Cheng X,Mao GP,Hu WJ,Yu ZR,Xu YY,Chen W,Li X,Zeng XL,Zhang WW,Chen JW,Wan Y,Wang L

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2023

Exosomes derived from stem cells of human deciduous exfoliated teeth inhibit angiogenesis in vivo and in vitro via the transfer of miR-100-5p and miR-1246.

Stem cell research & therapy

Liu P,Zhang Q,Mi J,Wang S,Xu Q,Zhuang D,Chen W,Liu C,Zhang L,Guo J,Wu X

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Thu Mar 03 00:00:00 EST 2022

Production enhancement of human adipose-derived mesenchymal stem cells by low-intensity ultrasound stimulation.

Scientific reports

Min S,Byeon Y,Kim M,Lee Y,Lee SH,Lee Y,Farooqi HMU,Lee HK,Paeng DG

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Wed Dec 21 00:00:00 EST 2022

Isolation and characterization mesenchymal stem cells from red panda (Ailurus fulgens styani) endometrium.

Conservation physiology

Wang DH,Wu XM,Chen JS,Cai ZG,An JH,Zhang MY,Li Y,Li FP,Hou R,Liu YL

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2022

Induced Neurodifferentiation of hBM-MSCs through Activation of the ERK/CREB Pathway via Pulsed Electromagnetic Fields and Physical Stimulation Promotes Neurogenesis in Cerebral Ischemic Models.

International journal of molecular sciences

Park HJ,Choi JH,Nam MH,Seo YK

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Fri Jan 21 00:00:00 EST 2022

Mesenchymal stem cells derived from patients with premature aging syndromes display hallmarks of physiological aging.

Life science alliance

Trani JP,Chevalier R,Caron L,El Yazidi C,Broucqsault N,Toury L,Thomas M,Annab K,Binetruy B,De Sandre-Giovannoli A,Levy N,Magdinier F,Robin JD

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Wed Sep 14 00:00:00 EDT 2022

Aberrant Expression of COX-2 and FOXG1 in Infrapatellar Fat Pad-Derived ASCs from Pre-Diabetic Donors.

Cells

O'Donnell BT,Monjure TA,Al-Ghadban S,Ives CJ,L'Ecuyer MP,Rhee C,Romero-Lopez M,Li Z,Goodman SB,Lin H,Tuan RS,Bunnell BA

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

The Effect of Different Doses of Melatonin on in Vitro Maturation of Human Follicular Fluid-Derived Oocyte-Like Cells.

JBRA assisted reproduction

Azandeh S,Moghadam MT,Rashno M,Zargar M,Zadeh PA

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Immunocytochemistry

Sun Apr 17 00:00:00 EDT 2022

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Scientific reports

Chen C,Huang X,Zhu W,Ding C,Huang P,Li R

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

Development, characterization, and hematopoietic differentiation of Griscelli syndrome type 2 induced pluripotent stem cells.

Stem cell research & therapy

Güney-Esken G,Erol ÖD,Pervin B,Gürhan Sevinç G,Önder T,Bilgiç E,Korkusuz P,Günel-Özcan A,Uçkan-Çetinkaya D,Aerts-Kaya F

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Human Amniotic Mesenchymal Stem Cells Inhibit aGVHD by Regulating Balance of Treg and T Effector Cells.

Journal of inflammation research

Gao Y,Li W,Bu X,Xu Y,Cai S,Zhong J,Du M,Sun H,Huang L,He Y,Hu X,Liu Q,Jin H,Wang Q,Ping B

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Tue Apr 26 00:00:00 EDT 2022

Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model.

Stem cell research & therapy

Li Y,Shi G,Han Y,Shang H,Li H,Liang W,Zhao W,Bai L,Qin C

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Thu Jul 15 00:00:00 EDT 2021

ZKSCAN3 counteracts cellular senescence by stabilizing heterochromatin.

Nucleic acids research

Hu H,Ji Q,Song M,Ren J,Liu Z,Wang Z,Liu X,Yan K,Hu J,Jing Y,Wang S,Zhang W,Liu GH,Qu J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2020

Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction.

Protein & cell

Wang S,Min Z,Ji Q,Geng L,Su Y,Liu Z,Hu H,Wang L,Zhang W,Suzuiki K,Huang Y,Zhang P,Tang TS,Qu J,Yu Y,Liu GH,Qiao J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

Resveratrol rescues TNF‑α‑induced inhibition of osteogenesis in human periodontal ligament stem cells via the ERK1/2 pathway.

Molecular medicine reports

Yuan J,Wang X,Ma D,Gao H,Zheng D,Zhang J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

Vasculogenesis from Human Dental Pulp Stem Cells Grown in Matrigel with Fully Defined Serum-Free Culture Media.

Biomedicines

Luzuriaga J,Irurzun J,Irastorza I,Unda F,Ibarretxe G,Pineda JR

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Mon Nov 09 00:00:00 EST 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

Chondrogenic Differentiation from Induced Pluripotent Stem Cells Using Non-Viral Minicircle Vectors.

Cells

Rim YA,Nam Y,Park N,Jung H,Lee K,Lee J,Ju JH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

The Effect of Angiotensin II, Retinoic Acid, EGCG, and Vitamin C on the Cardiomyogenic Differentiation Induction of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.

International journal of molecular sciences

Gasiūnienė M,Valatkaitė E,Navakauskaitė A,Navakauskienė R

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Thu Nov 19 00:00:00 EST 2020

Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages.

Stem cell research & therapy

Liu J,Qiu X,Lv Y,Zheng C,Dong Y,Dou G,Zhu B,Liu A,Wang W,Zhou J,Liu S,Liu S,Gao B,Jin Y

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Fri Nov 27 00:00:00 EST 2020

Up-regulation of FOXD1 by YAP alleviates senescence and osteoarthritis.

PLoS biology

Fu L,Hu Y,Song M,Liu Z,Zhang W,Yu FX,Wu J,Wang S,Izpisua Belmonte JC,Chan P,Qu J,Tang F,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer.

Cancers

Huang JY,Wang YY,Lo S,Tseng LM,Chen DR,Wu YC,Hou MF,Yuan SF

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Fri Dec 20 00:00:00 EST 2019

Better therapeutic potential of bone marrow-derived mesenchymal stem cells compared with chorionic villi-derived mesenchymal stem cells in airway injury model.

Regenerative medicine

Ji S,Wu C,Tong L,Wang L,Zhou J,Chen C,Song Y

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Differential stem cell aging kinetics in Hutchinson-Gilford progeria syndrome and Werner syndrome.

Protein & cell

Wu Z,Zhang W,Song M,Wang W,Wei G,Li W,Lei J,Huang Y,Sang Y,Chan P,Chen C,Qu J,Suzuki K,Belmonte JCI,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Antagonizing CD105 enhances radiation sensitivity in prostate cancer.

Oncogene

Madhav A,Andres A,Duong F,Mishra R,Haldar S,Liu Z,Angara B,Gottlieb R,Zumsteg ZS,Bhowmick NA

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2018

Utilizing Confocal Microscopy to Characterize Human and Mouse Adipose Tissue.

Tissue engineering. Part C, Methods

Blackshear CP,Borrelli MR,Shen EZ,Ransom RC,Chung NN,Vistnes SM,Irizarry D,Nazerali R,Momeni A,Longaker MT,Wan DC

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Immunohistochemistry

Mon Oct 01 00:00:00 EDT 2018

Utilizing Confocal Microscopy to Characterize Human and Mouse Adipose Tissue.

Tissue engineering. Part C, Methods

Blackshear CP,Borrelli MR,Shen EZ,Ransom RC,Chung NN,Vistnes SM,Irizarry D,Nazerali R,Momeni A,Longaker MT,Wan DC

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Immunohistochemistry

Mon Oct 01 00:00:00 EDT 2018

The Improvement of Respiratory Performance After Phototherapy-Induced EPC Mobilization in Preterm Infants With RDS.

Journal of cellular biochemistry

Siavashi V,Asadian S,Taheri-Asl M,Babaei H,Keshavarz S,Bazaei M,Nassiri SM

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Circulation Enrichment of Functional Endothelial Progenitor Cells by Infantile Phototherapy.

Journal of cellular biochemistry

Siavashi V,Asadian S,Sharifi A,Esmaeilivand M,Norouzinia R,Azadbakht M,Nassiri SM

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 64-1057-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: CD105; CD105 antigen; Endoglin; ORW

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Gene Aliases: END; ENG; HHT1; ORW1

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UniProt ID: (Human) P17813

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Entrez Gene ID: (Human) 2022

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Function(s)

transmembrane signaling receptor activity transforming growth factor beta receptor, cytoplasmic mediator activity type II transforming growth factor beta receptor binding protein binding galactose binding glycosaminoglycan binding type I transforming growth factor beta receptor binding BMP binding protein homodimerization activity activin binding transforming growth factor beta binding transmembrane signal receptor

Process(es)

chronological cell aging patterning of blood vessels vasculogenesis positive regulation of protein phosphorylation heart looping sprouting angiogenesis endocardial cushion morphogenesis cardiac ventricle morphogenesis cardiac atrium morphogenesis ventricular trabecula myocardium morphogenesis cell migration involved in endocardial cushion formation regulation of transcription, DNA-templated cell adhesion negative regulation of gene expression positive regulation of pathway-restricted SMAD protein phosphorylation regulation of transforming growth factor beta receptor signaling pathway central nervous system vasculogenesis negative regulation of cell migration BMP signaling pathway positive regulation of BMP signaling pathway negative regulation of protein autophosphorylation dorsal aorta morphogenesis positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter smooth muscle tissue development artery morphogenesis venous blood vessel morphogenesis endocardial cushion to mesenchymal transition positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD105 (Endoglin) Monoclonal Antibody (SN6), Super Bright™ 645, eBioscience™

CD105 (Endoglin) Antibody (64-1057-42) in Flow

Product Details

64-1057-42

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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