Invitrogen

CD10 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure

2 References

View all (160) CD10 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD10 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

CD10 Antibody (PA5-47075) in ICC/IF

Immunofluorescence analysis of CD10 was performed using LNCaP and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD10 Goat Polyclonal Antibody (Product # PA5-47075) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Rabbit anti-Goat IgG Heavy Chain Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27012) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green)... View More

CD10 Antibody in Immunohistochemistry (Frozen) (IHC (F))

CD10 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD10 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of CD10 was observed in LNCaP (Lane 1), Raji (Lane 3), Ramos (Lane 4) cell lines that express CD10, in comparison to PC-3 (Lane 2) and HeLa (Lane 5) cell lines that are null for CD10 expression. Relative expression was also observed in Mouse Kidney and Mouse Lungs (Lanes 6 & 7) that express high levels of CD10 protein, in comparison to Mouse Heart and Mouse Skeletal Muscle (Lanes 8 & 9) that express extremely low levels, using Anti-CD10 Goat Polyclonal Antibody (Product # PA5-47075) in Western Blot. {RE}

Product Details

PA5-47075

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL

Immunocytochemistry (ICC/IF)

5 µg/ml

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 0.2-0.8 µg/mL (Capture)

Immunoprecipitation (IP)

25 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Sf 21-derived recombinant mouse Neprilysin/CD10 Tyr52-Trp750

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2607390

Product Specific Information

In sandwich ELISAs, less than 35% cross-reactivity with recombinant human (rh) Neprilysin is observed and less than 0.2% cross-reactivity with rhNeprilysin-2 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

CD10 (Common Acute Lymphocytic Leukemia Antigen, CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells. CD10 is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. Further, CD10 is a 100 kDa type II transmembrane glycoprotein that exists in a single copy of greater than 45 kb. The 5' untranslated region of the CD10 gene is alternatively spliced, resulting in four separate mRNA transcripts and the coding region is not affected by alternative splicing. Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie tooth Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HIF-1α and HIF-2α differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice.

Nature communications

Hoefflin R,Harlander S,Schäfer S,Metzger P,Kuo F,Schönenberger D,Adlesic M,Peighambari A,Seidel P,Chen CY,Consenza-Contreras M,Jud A,Lahrmann B,Grabe N,Heide D,Uhl FM,Chan TA,Duyster J,Zeiser R,Schell C,Heikenwalder M,Schilling O,Hakimi AA,Boerries M,Frew IJ

PA5-47075 was used in Immunohistochemistry to show using an autochthonous ccRCC model that Hif1a is essential for tumour formation whereas Hif2a deletion has only minor effects on tumour initiation and growth.

Mon Aug 17 00:00:00 EDT 2020

Oncogenic HrasG12V expression plus knockdown of Cdkn2a using ecotropic lentiviral vectors induces high-grade endometrial stromal sarcoma.

PloS one

Brandt LP,Albers J,Hejhal T,Catalano A,Wild PJ,Frew IJ

Published figure using CD10 polyclonal antibody (Product # PA5-47075) in Immunohistochemistry

Fri Nov 03 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Atriopeptidase; CALLA; CD10; common acute lymphoblastic leukemia antigen; Common acute lymphocytic leukemia antigen; Enkephalinase; membrane metallo endopeptidase; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); membrane metallo-endopeptidase (neutral endopeptidase/enkephalinase); membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; NEP; Neprilysin; neprilysin-390; neprilysin-411; Neutral endopeptidase 24.11; SFE; Skin fibroblast elastase

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Gene Aliases: 6030454K05Rik; C85356; CALLA; CD10; CMT2T; EPN; MME; NEP; SFE

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UniProt ID: (Human) P08473, (Mouse) Q61391, (Rat) P07861

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Entrez Gene ID: (Human) 4311, (Mouse) 17380, (Rat) 24590

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Function(s)

endopeptidase activity metalloendopeptidase activity protein binding metallopeptidase activity exopeptidase activity zinc ion binding peptide binding peptidase activity hydrolase activity metal ion binding metalloprotease

Process(es)

kidney development angiotensin maturation proteolysis peptide metabolic process sensory perception of pain creatinine metabolic process beta-amyloid metabolic process cellular response to cytokine stimulus cellular response to UV-A cellular response to UV-B replicative senescence

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD10 Polyclonal Antibody

CD10 Antibody (PA5-47075) in ICC/IF

Product Details

PA5-47075

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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