Invitrogen

CALM Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Reference

View all (16) CALM antibodies

Datasheet

Protocols

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Datasheet

Protocols

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CALM Antibody (PA5-82661)

Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of CALM was observed specifically in Caco-2 cells and in PC-3 cells using anti-CALM Polyclonal Antibody (Product #PA5-82661) in western blot. The relative expression levels of CALM within each cell line is shown using RNA-Seq. {RE}

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CALM Antibody — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of CALM was observed specifically in Caco-2 cells and in PC-3 cells using anti-CALM Polyclonal Antibody (Product #PA5-82661) in western blot. The relative expression levels of CALM within each cell line is shown using RNA-Seq. {RE}

Product Details

PA5-82661

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PICALM. Recombinant protein control fragment (Product #RP-92550).

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Anitgen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2789817

Product Specific Information

Immunogen sequence: DIPDLSQAPS SLLDALEQHL ASLEGKKIKD STAASRATTL SNAVSSLAST GLSLTKVDER EKQAALEEEQ ARLKALKEQR L

Target Information

PICALM, also known as the clathrin assembly lymphoid myeloid leukemia (CALM) protein, co-localizes with clathrin at clathrin-coated areas of the plasma membrane and in the Golgi area, and is thought to be an important component of the coated pit internalization machinery. Fusion proteins involving PICALM have been implicated in acute myeloid and T-cell acute lymphoblastic leukemia. PICALM is also expressed in neurons and is present at the synapse where it facilitates the endocytosis of the synaptic vesicle protein VAMP2. Recent studies have identified variants of PICALM associated with Alzheimer's disease and have suggested this protein may play a role in the endocytosis and recycling of the amyloid protein precursor (APP).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats.

Frontiers in nutrition

Long X,Wu H,Zhou Y,Wan Y,Kan X,Gong J,Zhao X

PA5-82661 was used in Western Blotting to show that L. fermentum SCHY34 showed a great biological scavenging and potential effect on alleviating the toxicity of lead ions.

Sat Jul 16 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: CALM; Clathrin assembly lymphoid myeloid leukemia; Clathrin assembly lymphoid myeloid leukemia protein; clathrin-assembly lymphoid leukemia protein; clathrin-assembly lymphoid myeloid leukemia protein; Phosphatidylinositol-binding clathrin assembly protein; rCALM

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Gene Aliases: CALM; CLTH; fit-1; Fit1; LAP; mKIAA4114; PAP180; PICALM

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UniProt ID: (Human) Q13492, (Mouse) Q7M6Y3, (Rat) O55012

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Entrez Gene ID: (Human) 8301, (Mouse) 233489, (Rat) 89816

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Function(s)

protein binding 1-phosphatidylinositol binding clathrin binding clathrin heavy chain binding clathrin adaptor activity cadherin binding involved in cell-cell adhesion phospholipid binding SH3 domain binding identical protein binding vesicle coat protein

Process(es)

protein complex assembly receptor-mediated endocytosis cell proliferation negative regulation of gene expression synaptic vesicle maturation vesicle-mediated transport endosomal transport regulation of endocytosis receptor internalization regulation of protein localization cargo loading into vesicle positive regulation of transcription, DNA-templated negative regulation of receptor-mediated endocytosis clathrin coat assembly iron ion homeostasis clathrin-mediated endocytosis iron ion import into cell cell-cell adhesion positive regulation of neuron death positive regulation of beta-amyloid formation regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process endocytosis multicellular organism development axonogenesis hemopoiesis dendrite morphogenesis regulation of protein transport

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CALM Polyclonal Antibody

CALM Antibody (PA5-82661)

Product Details

PA5-82661

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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